ABSTRACT
Crystallization tests are performed with porcine insulin previously purified by single chromatography to produce Lente insulins. The following parameters are changed under the test: crystallization temperature, stirring rate, pH of crystallization, and the period from the beginning of insulin precipitation until the isoelectric point is reached. The effects of these parameters on crystallization kinetics, crystal size and crystal size distribution are being studied.
Subject(s)
Insulin/chemistry , Animals , Buffers , Crystallization , Hydrogen-Ion Concentration , Insulin/isolation & purification , SwineABSTRACT
A method is described for the quantitative determination of amorphous and crystalline insulin contents in insulin zinc suspensions. The different resolving rates of the modifications in acid dilutions are utilized for the test. Measurements are made by UV spectroscopy. Information is given on the application of this method for the analysis of the course of insulin crystallization and it is compared with the USP XXI method.
Subject(s)
Insulin/analysis , Crystallization , Spectrophotometry, Ultraviolet , Suspensions , Zinc/analysisABSTRACT
With respect to their performance a computer aided spectrometric analysis of multicomponent system dihydralazine sulfate hydrochlorothiazide and reserpine was made under various conditions. To find out the advantageous measuring points the difference spectra were determined. The influence of the number of measuring points, the single and mixture calibration and the dimensiation of calibration matrix to accuracy and precision was investigated and the limits of the determination for the three component system are discussed.
