Serum vitamin D in sanctuary chimpanzees (Pan troglodytes) in range countries: A pilot study.

BACKGROUND: Vitamin D is essential for skeletal health, calcium homeostasis and general health. The major and more stable form of vitamin D in circulation is 25-hydroxyvitamin D (25-OH-D); this is the most valuable indicator of vitamin D status. There are studies on laboratory and zoo-housed chimpanzees; however, serum vitamin D status has not been documented in chimpanzees in range countries. OBJECTIVES: (1) Determine the range of circulating 25-OH-D concentrations in chimpanzees in range countries. (2) Assess the influence of age, sex, and sun exposure on 25-OH-D serum concentrations. METHODS: Opportunistic blood samples were obtained from 127 clinically healthy chimpanzees. Serum 25-OH-D concentration was measured with a commercially available competitive ELISA. RESULTS: The median overall 25-OH-D concentration for chimpanzees in range countries was 46.24 nmol/L (range: 17.10-109.23 nmol/L). Males had a significantly lower concentration (40.15 nmol/L) than females (49.61 nmol/L), and infants (37.99 nmol/L) had a significantly lower concentration than adults (46.04 nmol/L). Concentrations of 25-OH-D in chimpanzees in sunnier habitats were significantly higher compared to thick tropical forest habitat. CONCLUSION: The present constitutes a large dataset of serum 25-OH-D concentrations in range country sanctuary chimpanzees and contributes to document normal ranges. Age, sex, and sun exposure influenced serum concentrations of 25-OH-D in sanctuary chimpanzees.

Reproductive one health in primates.

One Health is a collaborative trans-disciplinary approach to health; integrating human, animal, and environmental health. The focus is often on infection disease transmission and disease risk mitigation. However, One Health also includes the multidisciplinary and comparative approach to disease investigation and health of humans, animals, and the environment. One key aspect of environmental/ecosystem health is conservation, the maintenance of healthy, actively reproducing wildlife populations. Reproduction and reproductive health are an integral part of the One Health approach: the comparative aspects of reproduction can inform conservation policies or breeding strategies (in situ and ex situ) in addition to physiology and disease. Differences in reproductive strategies affect the impact poaching and habitat disruption might have on a given population, as well as ex situ breeding programs and the management of zoo and sanctuary populations. Much is known about chimpanzees, macaques, and marmosets as these are common animal models, but there is much that remains unknown regarding reproduction in many other primates. Examining the similarities and differences between and within taxonomic groups allows reasonable extrapolation for decision-making when there are knowledge gaps. For example: (1) knowing that a species has very low reproductive rates adds urgency to conservation policy for that region or species; (2) identifying species with short or absent lactation anestrus allows ex situ institutions to better plan contraception options for specific individuals or prepare for the immediate next pregnancy; (3) recognizing that progestin contraceptives are effective contraceptives, but may be associated with endometrial hyperplasia in some species (in Lemuridae but not great apes) better guides empirical contraceptive choice; (4) recognizing the variable endometriosis prevalence across taxa improves preventive medicine programs. A summary of anatomical variation, endocrinology, contraception, pathology, and diagnostics is provided to illustrate these features and aid in routine physical and postmortem examinations as well as primate management.

EFFECTS OF A GNRH VACCINE (IMPROVAC®) ON PATAGONIAN (OTARIA BYRONIA) AND CALIFORNIA SEA LIONS (ZALOPHUS CALIFORNIANUS).

Improvac® is a gonadotropin-releasing hormone vaccine developed to reduce "boar taint" in the meat of male domestic pig. The use of Improvac for contraception of zoo and free-living animals has been increasing in recent years. This study reports the use, efficacy, and side effects of Improvac on five male sea lions. Administration of two injections of 600 µg of Improvac (gonadotropin releasing factor analogue-protein conjugate) 4-5 wk apart were delivered to two Patagonian and three California sea lions to reduce testosterone-related aggression, anorexia, and lethargy that occur during the breeding season. Behavior and physical changes were recorded for all individuals, and blood samples were taken from one Patagonian sea lion to measure plasma testosterone concentrations over time. Observations revealed a descension of the testes into the scrotum, orchitis, lameness, anorexia, and lethargy in all individuals for the first 3-5 d after the first administration of the vaccine. Plasma testosterone concentrations rose after the first dose of the vaccine and remained elevated for 1 mo, decreasing after the second injection to undetectable levels. Improvac administration can cause a peak of testosterone and breeding behavior just after the first inoculation, as previously described in swine and elephants, but has not been documented in pinnipeds. None of the treated animals in this study showed breeding behaviors during their normal breeding season (July-September).