ABSTRACT
BACKGROUND: Although using pathological diagnosis is the gold standard for lung cancer diagnosis, pathological reports take a long time. Rapid on-site assessment (ROSE) is a method to determine whether the quality of the specimens is sufficient for cytopathological diagnosis, which issues a preliminary report during the operation and takes shorter time. The aim of this study is to explore the clinical significance of rapid on-site evaluation (ROSE) in the diagnosis of lung cancer in terms of real-time and accuracy. METHODS: A total of 38 suspected lung cancer cases were enrolled from August 2019 to July 2020. Patients received ROSE and pathological examinations at the same time. The coincidence rate of the two diagnostic approaches was calculated, and statistical analysis was carried out to evaluate whether the time difference between the ROSE report and the pathological report reached statistical significance. RESULTS: A total of 38 suspected lung cancer cases were enrolled from August 2019 to July 2020. Patients received ROSE and pathological examinations at the same time. The coincidence rate of the two diagnostic approaches was calculated, and statistical analysis was carried out to evaluate whether the time difference between the ROSE report and the pathological report reached statistical significance.. CONCLUSIONS: ROSE has the advantage of rapid and accurate diagnosis for lung cancer, and has great clinical significance.
ABSTRACT
OBJECTIVE: To investigate the role of CXXC5 and the CD40/CD40L pathway in lung fibrosis. METHODS: (1) We constructed mouse models of bleomycin-induced pulmonary fibrosis and transfected them with a CXXC5 overexpression vector to evaluate the severity of pulmonary fibrosis. (2) Mouse lung fibroblast (MLF) models stably overexpressed or knockout of CXXC5 vector were constructed. After transforming growth factor-ß1 (TGF-ß1) stimulation, we examined the proliferation and apoptosis of the MLF model and evaluated the expression of mesenchymal markers and the CXXC5/CD40/CD40L pathway. RESULTS: (1) Compared with other groups, the overexpressed CXXC5 group had less alveolar structure destruction, thinner alveolar septum, and lower Ashcroft score. (2) In bleomycin-induced mice, the expression of CD40 and CD40L increased at both transcriptional and protein levels, and the same changes were observed in α-smooth muscle actin (α-SMA) and collagen type I (Colla I). After upregulation of CXXC5, the increase in CD40, CD40L, α-SMA, and Colla I was attenuated. (3) Stimulated with TGF-ß1, MLF proliferation was activated, apoptosis was suppressed, and the expression of CD40, CD40L, α-SMA, and Colla I was increased at both transcriptional and protein levels. After upregulation of CXXC5, these changes were attenuated. CONCLUSION: CXXC5 inhibits pulmonary fibrosis and transformation to myofibroblasts by negative feedback regulation of the CD40/CD40L pathway.
