ABSTRACT
Objective:To investigate the effect of transoral endoscopic thyroid surgery in the central lymph node dissection of thyroid cancer. Methods:Twenty patients underwent endoscopic thyroidectomy via oral vestibular approach ï¼TOETVA groupï¼ and 20 gender and age matched patients underwent conventional open thyroidectomy were selected in this study. The clinical data, number of lymph node dissection and postoperative complications of the two groups were collected and analyzed. SPSS 24.0 statistical software was used for data analysis. Results:There was no significant differences in age, BMI, tumor size or the number of Hashimoto's thyroiditis patients between the TOETVA group and the open groupï¼P>0.05ï¼. The operation timeï¼[117.30±10.54]minï¼ and postoperative drainage volumeï¼[146.05±30.66]mLï¼ in the TOETVA group were significantly higher than those in the open groupï¼[59.05±6.40]min, [77.90±22.18]mLï¼, P<0.001. The pain in the open group 24 h after surgery was more severe than that in the TOETVA groupï¼P<0.05ï¼. The total number of central lymph nodes, the number of pre-laryngeal lymph nodes and the number of pre-tracheal lymph nodes cleared by the two groups were similar, and the number of final positive lymph nodes had no significant difference. However, the number of paratracheal lymph nodes removed in the TOETVA group was moreï¼7.8±4.2ï¼ nodes than that in the open groupï¼6.4±3.9ï¼ nodes, P<0.05. There was no significant difference in postoperative complications between the two groups. The scores of cosmetic evaluation and rating of cicatrices in the TOETVA group six months after surgery wereï¼0.77±0.58ï¼ significantly lower than those in the open groupï¼4.30±1.54ï¼, P<0.001. Conclusion:In central lymph node dissection, the efficiency of TOETVA is similar to that of traditional open thyroid surgery. For special anatomical regions, endoscopic surgery is more efficient. At the same time, TOETVA does not increase the incidence of related complications, and has good cosmetic effect.
Subject(s)
Thyroid Neoplasms , Humans , Neck Dissection , Postoperative Complications/surgery , Thyroid Neoplasms/pathology , Thyroid Neoplasms/surgery , ThyroidectomyABSTRACT
OBJECTIVES: To identify key long non-coding (lnc)RNAs responsible for the epithelial-mesenchymal transition (EMT) of CNE1 nasopharyngeal carcinoma cells and to investigate possible regulatory mechanisms in EMT. METHODS: CNE1 cells were divided into transforming growth factor (TGF)-ß1-induced EMT and control groups. The mRNA and protein expression of EMT markers was determined by real-time quantitative PCR and western blotting. Differentially expressed genes (DEGs) between the two groups were identified by RNA sequencing analysis, and DEG functions were analyzed by gene ontology and Kyoto Encyclopedia of Genes and Genomes analyses. EMT marker expression was re-evaluated by western blotting after knockdown of a selected lncRNA. RESULTS: TGF-ß1-induced EMT was characterized by decreased E-cadherin and increased vimentin, N-cadherin, and Twist expression at both mRNA and protein levels. Sixty lncRNA genes were clustered in a heatmap, and mRNA expression of 14 dysregulated lncRNAs was consistent with RNA sequencing. Knockdown of lnc-PNRC2-1 increased expression of its antisense gene MYOM3 and reduced expression of EMT markers, resembling treatment with the TGF-ß1 receptor inhibitor LY2109761. CONCLUSION: Various lncRNAs participated indirectly in the TGF-ß1-induced EMT of CNE1 cells. Lnc-PNRC2-1 may be a key regulator of this and is a potential target to alleviate CNE1 cell EMT.
