ABSTRACT
Objective: To compare the clinical outcomes among three kinds of transforaminal interbody fusion technique in the treatment of single segment lumbar degenerative disease. Methods: From October 2012 to November 2014, a total of 67 cases of lumbar degenerative disease of single segment underwent surgical intervention were retrospectively analyzed, including 19 cases by mis- transforaminal lumbar interbody fusion(TLIF), 25 cases by mini-open TLIF and 23 cases by open TLIF.Blood loss, surgical time, fluoroscopy time and hospital costs were recorded.Pre- and postoperative back pain was assessed with visual analogue scale (VAS), and lumbar function was evaluated with Oswestry disability index (ODI). Results: mis-TLIF group need more surgical time, fluoroscopy time and more hospital costs when compared to mini-open TLIF group and open TLIF group (P<0.05), but no significant difference between mini-open TLIF group and open TLIF group (P>0.05). mis-TLIF group tended to lose less blood than mini-open TLIF group(P<0.05), and mini-open TLIF group lose less blood than open TLIF group (P<0.05). There is no significant difference of the change of ODI among three groups (P>0.05). mis-TLIF group and mini-open TLIF group was superior to open TLIF group in VAS of the second day postoperative [(3.2±1.6) scale, (3.4±1.5) scale vs(5.8±1.4)scale](P<0.05). Conclusion: mis-TLIF, mini-open TLIF and open TLIF can all get satisfactory clinical outcomes in the treatment of single segment lumbar degenerative disease.mis-TLIF and mini-open TLIF was superior to open TLIF in blood loss, early postoperative recovery.Compared with mis-TLIF, mini-open TLIF reduce the fluoroscopy time and hospital costs, and also has a shorter learning curve.
Subject(s)
Lumbar Vertebrae , Spinal Diseases/surgery , Spinal Fusion , Fluoroscopy , Humans , Lumbosacral Region , Minimally Invasive Surgical Procedures , Pain Measurement , Pain, Postoperative , Postoperative Period , Retrospective StudiesABSTRACT
Here, polycythemia vera (PV)-related genes were screened by the Online Mendelian Inheritance in Man (OMIM), and literature pertaining to the identified genes was extracted and a protein-protein interaction network was constructed using various Cytoscape plugins. Various molecular complexes were detected using the Clustervize plugin and a gene ontology-enrichment analysis of the biological pathways, molecular functions, and cellular components of the selected molecular complexes were identified using the BiNGo plugin. Fifty-four PV-related genes were identified in OMIM. The protein-protein interaction network contains 5 molecular complexes with correlation integral values >4. These complexes regulated various biological processes (peptide tyrosinase acidification, cell metabolism, and macromolecular biosynthesis), molecular functions (kinase activity, receptor binding, and cytokine activity), and the cellular components were mainly concentrated in the nucleus, intracellular membrane-bounded organelles, and extracellular region. These complexes were associated with the JAK-STAT signal transduction pathway, neurotrophic factor signaling pathway, and Wnt signaling pathway, which were correlated with chronic myeloid leukemia and acute myeloid leukemia.
Subject(s)
Gene Regulatory Networks , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/genetics , Leukemia, Myeloid, Acute/genetics , Metabolic Networks and Pathways/genetics , Polycythemia Vera/genetics , Protein Interaction Mapping , Databases, Genetic , Gene Expression Regulation , Gene Ontology , Humans , Janus Kinase 1/genetics , Janus Kinase 1/metabolism , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/metabolism , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/pathology , Leukemia, Myeloid, Acute/metabolism , Leukemia, Myeloid, Acute/pathology , Molecular Sequence Annotation , Polycythemia Vera/metabolism , Polycythemia Vera/pathology , STAT Transcription Factors/genetics , STAT Transcription Factors/metabolism , Signal Transduction , Wnt Proteins/genetics , Wnt Proteins/metabolismABSTRACT
The objective of this study was the development of a gene/protein interaction network for primary myelofibrosis based on gene expression, and the enrichment analysis of KEGG pathways underlying the molecular complexes in this network. To achieve this, genes involved in primary myelofibrosis were selected from the OMIM database. A gene/protein interaction network for primary myelofibrosis was obtained through Cytoscape with the literature mining performed using the Agilent Literature Search plugin. The molecular complexes in the network were detected by ClusterViz plugin and KEGG pathway enrichment of molecular complexes was performed using DAVID online. We found 75 genes associated with primary myelofibrosis in the OMIM database. The gene/protein interaction network of primary myelofibrosis contained 608 nodes, 2086 edges, and 4 molecular complexes with a correlation integral value greater than 4. Molecular complexes involved in KEGG pathways are related to cytokine regulation, immune function regulation, ECM-receptor interaction, focal adhesion, actin cytoskeleton regulation, cell adhesion molecules, and other biological behavior of tumors, which can provide a reliable direction for the treatment of primary myelofibrosis and the bioinformatic foundation for further understanding the molecular mechanisms of this disease.
