OsGLP8-7 interacts with OsPRX111 to detoxify excess copper in rice.

Lignin is a phenolic biopolymer generated from phenylpropanoid pathway in the secondary cell wall and is required for defense of plants against various stress. Although the fact of stress-induced lignin deposition has been clearly demonstrated, it remains largely elusive how the formation of lignin is promoted under Cu stress. The present study showed that OsGLP8-7, an extracellular glycoprotein of rice (Oryza sativa L.), plays an important function against Cu stress. The loss function of OsGLP8-7 results in Cu sensitivity whereas overexpression of OsGLP8-7 scavenges Cu-induced superoxide anion (O2•-). OsGLP8-7 interacts with apoplastic peroxidase111 (OsPRX111) and elevates OsPRX111 stability when exposed to excess Cu. In OsGLP8-7 overexpressing (OE) lines, the retention of Cu within cell wall limiting Cu uptake into cytoplasm is attributed to the enhanced lignification required for Cu tolerance. Exogenous application of a lignin inhibitor can impair the Cu tolerance of transgenic Arabidopsis lines overexpressing OsGLP8-7. In addition, co-expression of OsGLP8-7 and OsPRX111 genes in tobacco leaves leads to an improved lignin deposition compared to leaves expressing each gene individually or the empty vector. Taken together, our findings provided the convincing evidences that the interaction between OsGLP8-7 and OsPRX111 facilitates effectively lignin polymerization, thereby contributing to Cu tolerance in rice.

[Research of preferences and security management of tourists in Poyang Lake based on schistosomiasis prevention].

OBJECTIVE: To discuss the prevention of schistosomiasis in tourism of lake region. METHODS: The seasonal distribution of tourism activities and spatial distribution of scenic spots, as well as the coupling between space and temporal of Oncomelania snail distribution and the transmission time of schistosomiasis in Poyang Lake region were analyzed. The travel preference of schistosomiasis susceptible population was surveyed by questionnaires and interviews. RESULTS: There were couplings of space and temporal between tourism activities in Poyang Lake region and transmission time of schistosomiasis as well as space distribution of snails, respectively. The most popular tourism items were Shuishangrenjia (overwater household) and fishing folk culture with property of participation and experience. CONCLUSION: The suggestion is to establish health records of tourists, carry out health education of schistosomiasis, and enhance the management of tourism and activities of tourists.

Two new 11(15-->1)abeotaxanes with a 2,20-epoxy ring from the needles of Taxus canadensis.

Two 11(15-->1)abeotaxanes having a tetrahydrofuran ring along the carbon atoms C-2, C-3, C-4, C-20 were identified for the first time from the needles of the Canadian yew, Taxus canadensis. The compounds could be identified as 4alpha,10beta,13alpha-triacetoxy-15-benzoyloxy-2alpha,20beta-epoxy-11(15-->1)abeotax-11-ene-5alpha,7beta,9alpha-triol (1) and 4alpha,7beta,9alpha,10beta,15-pentaacetoxy-2alpha,20beta-epoxy-11(15-->1)abeotax-11-ene-5alpha,13alpha-diol (2) on the basis of 1D-, 2D-NMR evidence and high-resolution FABMS analysis. Compound 1 showed weak growth inhibitory activities against T-98 and MM1-CB cells in vitro.