A novel fluorescence molecularly imprinted sensor for Japanese encephalitis virus detection based on metal organic frameworks and passivation-enhanced selectivity.

Accurate detection of viruses is of great significance in preventing further spreading of infections and developing appropriate clinical treatment. Herein, a fluorescence molecularly imprinted sensor based on a metal-organic framework with high selectivity and high sensitivity at concentrations down to the picomolar (pmol) level was developed to recognize Japanese encephalitis virus (JEV). In this work, zinc acrylate was used as the functional monomer to form molecularly imprinted polymers on the surface of a silicon-modified metal organic frameworks via free radical polymerization. Polyethylene glycol (PEG) was then used as a blocking agent to enhance the ability of polymers to specifically recognize the template virus. Under optimal experimental conditions, the polymers exhibit a wide range of detection, 50 pmol L-1 to 1400 pmol L-1, within 20 min, a low detection limit (13 pmol L-1), and good selectivity (IF = 4.3). These advantages enable this molecularly imprinted (MIP) sensor for important practical application value and significance in the detection and prevention of viruses.

Molecularly imprinted polymer based hybrid structure SiO2@MPS-CdTe/CdS: a novel fluorescence probe for hepatitis A virus.

A novel designed fluorescence molecularly imprinted polymer (MIP) probe made from CdTe/CdS quantum dot (QD)-based silica nanoparticles (SiO2@MPS-CdTe/CdS) was successfully created via a sol-gel process. The target virus - hepatitis A virus (HAV) was selectively captured by imprinted polymer layer, resulting in the fluorescence quenching of the QDs within 20 min, which could be explained by the energy transfer mechanism. Under optimized conditions, the limit of detection for the SiO2@MPS-CdTe/CdS MIP was as low as 88 pmol · L-1, and excellent linearity was obtained from 0.2 to 1.4 nM. Additionally, the nanosensor was successfully used to detect the HAV from a dilution of human serum, in which recoveries were in the range of 96.7%-103.8%. Overall, the current work proposes a novel and cost-effective method to synthesize SiO2@MPS-CdTe/CdS MIPs for use as a tool to rapidly and efficiently detect HAV, and it also provides promising perspectives to further advance virus imprinting research.