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1.
RSC Adv ; 12(42): 27380-27388, 2022 Sep 22.
Article in English | MEDLINE | ID: mdl-36275998

ABSTRACT

Hydroxyl-terminated linear and star-shaped telechelic polyisobutylene have been successfully synthesized by living cationic polymerization using propylene oxide (PO)/Titanium tetrachloride (TiCl4) as the initiator system. A one-step method to prepare the terminal hydroxyl group was realized by selecting the cheap and beautiful epoxide as the functional initiator, which has the prospect of industrial application. The polymerization mechanism was proposed by the end structure analysis and Gaussian calculation results. At the same time, the living linear macromolecular chain was used as the starting point to react with divinyl compounds for synthesis of star-shaped hydroxyl-terminated polyisobutylene. The effects of initiator-crosslinking agent ratio, arm length, and reaction time on the coupling reaction were studied.

2.
Genes (Basel) ; 13(9)2022 08 24.
Article in English | MEDLINE | ID: mdl-36140687

ABSTRACT

Simple sequence repeats (SSRs) are one of the most important molecular markers, which are widespread in plants. Bamboos are important forest resources worldwide. Here, the comprehensive identification and comparative analysis of SSRs were performed in three woody and two herbaceous bamboo species. Altogether 567,175 perfect SSRs and 71,141 compound SSRs were identified from 5737.8 Mb genome sequences of five bamboo species. Di-nucleotide SSRs were the most predominant type, with an average of ~50,152.2 per species. Most SSRs were located in intergenic regions, while those located in genic regions were relatively less. Moreover, the results of annotation distribution indicated that terms with P450, peroxidase and ATP-binding cassette transporter related to lignin biosynthesis might play important roles in woody and herbaceous bamboos under the mediation of SSRs. Furthermore, the peroxidase gene family consisted of a large number of genes containing SSRs was selected for the evolutionary relationship analysis and SSR markers development. Fifteen SSR markers derived from peroxidase family genes of Phyllostachys edulis were identified as polymorphic in 34 accessions belonging to seven genera in Bambusoideae. These results provided a comprehensive insight of SSR markers into bamboo genomes, which would facilitate bamboo research related to comparative genomics, evolution and marker-assisted selection.


Subject(s)
Lignin , Microsatellite Repeats , ATP-Binding Cassette Transporters/genetics , DNA, Intergenic , Microsatellite Repeats/genetics , Nucleotides , Peroxidases/genetics
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