ABSTRACT
A strengthen circulation anaerobic reactor (SCAR) treating artificial municipal wastewater was investigated under different volumetric loading rate(VLR) and the reactor performance, characteristics of granular sludge and microbial community structure were also tested in this experiment. The results of the experiment demonstrated that the hydraulic retention time (HRT) of 6h could be regarded as the key parameter dominating the efficient operation of SCAR reactor, in which condition the COD removal efficiency was above 75%. The coenzyme F420and the maximum specific methanogenic activity (SMA) of granular sludge increased with increasing VLR, and the EPS contents, especially TB-EPS in the granule sludge also increased obviously. Consistently, the characteristics of anaerobic granular sludge and the removal efficiency of the reactor were influenced by both sludge loading and HRT. The microbial community structure and its spatial distribution in the reactor were also affected by sludge loading, while the relative abundance of the microbial community with different metabolic characteristics in different spatial positions changed with the adjustment of the sludge loading.
Subject(s)
Bioreactors/microbiology , Sewage/microbiology , Waste Disposal, Fluid , Wastewater , AnaerobiosisABSTRACT
The antierbB2 scFvFcIL2 fusion protein (HFI) is the basis for development of a novel targeted anticancer drug, in particular for the treatment of HER2positive cancer patients. HFI was fused with the antierbB2 antibody and human IL2 by genetic engineering technology and by antibody targeting characteristics of HFI. IL2 was recruited to target cells to block HER2 signaling, inhibit or kill tumor cells, improve the immune capacity, reduce the dose of antibody and IL2 synergy. In order to analyse HFI drug ability, HFI plasmid stability was verified by HFI expression of the trend of volume changes. Additionally, HFI could easily precipitate and had progressive characteristics and thus, the buffer system of the additive phosphatecitric acid buffer, arginine, Triton X100 or Tween80, the establishment of a microfiltration, ion exchange, affinity chromatography and gel filtration chromatographybased purification process were explored. HFI samples were obtained according to the requirements of purity, activity and homogeneity. In vivo, HFI significantly delayed HER2 overexpression of nonsmall cell lung cancer (Calu3) in human nonsmall cell lung cancer xenografts in nude mice, and the inhibition rate was more than 60% (P<0.05) in the group treated with 1 mg/kg the HFI dose; HFI significantly inhibited HER2 expression of breast cancer (FVB/neu) transgenic mouse tumor growth in 1 mg/kg of the HFI dose group, and in the following treatment the 400 mm3 tumors disappeared completely. Combined with other HFI test data analysis, HFI not only has good prospects, but also laid the foundation for the development of antibodycytokine fusion proteinlike drugs.
