Subject(s)
Multiple Myeloma , Renal Insufficiency , Humans , Multiple Myeloma/complications , Multiple Myeloma/diagnosis , Multiple Myeloma/drug therapy , Bortezomib/adverse effects , Thalidomide/adverse effects , Renal Insufficiency/complications , Renal Insufficiency/diagnosis , Dexamethasone/adverse effectsABSTRACT
The DFT-B3LYP and MP2 methods with 6-311G** and 6-311++G** basis sets have been applied to study the complexation energies of the host-guest complexes between the cone calix[4]arene and Li(+) or Na(+) on the B3LYP optimized geometries. A comparison of the complexation energies obtained from the MP2(full) with those from MP2(fc) method is also carried out. The result shows that it is essential to introduce the diffuse basis set into the geometry optimizations and complexation energy calculations of the alkali-metal cation-pi interaction complexes of calix[4]arene, and the D (e) values show a maximum of 21.13 kJ mol(-1) (14.45% of relative error) between the MP2(full)/6-311++G** and MP2(fc)/6-311++G** method. For Li(+) cation, the complexation is mainly energetically stabilized by the lower rim/cation (namely O-Li(+)) interaction. However, binding energies and NBO analyses confirm that Na(+) cation prefers to enter the calix[4]arene cavity and the cation-pi interaction is predominant, which contradicts the previous low-level theoretical studies. Furthermore, the complexation with Li(+) is preferred over that with Na(+) by at least 12.70 kJ mol(-1) at MP2(full)/6-311++G**//B3LYP/6-311++G** level.
Subject(s)
Calixarenes/chemistry , Lithium/chemistry , Models, Molecular , Sodium/chemistry , Molecular Conformation , ThermodynamicsABSTRACT
Recent studies in mice have shown that resveratrol can protect the liver from fat accumulation induced by high fat diet. However, the exact mechanism is largely unknown. To explore the possible mechanism, we investigated the anti-lipogenic effect of resveratrol in vitro model. Oil Red O staining revealed that resveratrol could significantly ameliorate the excessive triglyceride accumulation in HepG2 cells induced by palmitate. The results of RT-PCR and Western blotting showed that resveratrol upregulated the expression of Sirt1 and forkhead box O1 (FOXO1), whereas downregulated the expression of sterol regulatory element binding protein1 (SREBP1). Moreover, resveratrol was shown to inhibit the activity of SREBP1, as evaluated by immunofluorescence assay. Our results suggest that resveratrol may attenuate fat deposition by inhibiting SREBP1 expression via Sirt1-FOXO1 pathway and thus may have application for the treatment of NAFLD.
Subject(s)
Fatty Liver/metabolism , Hypolipidemic Agents/pharmacology , Sterol Regulatory Element Binding Protein 1/antagonists & inhibitors , Stilbenes/pharmacology , Triglycerides/metabolism , Animals , Caloric Restriction , Cell Line, Tumor , Cell Survival/drug effects , Forkhead Box Protein O1 , Forkhead Transcription Factors/metabolism , Humans , Mice , Models, Biological , Palmitic Acid/pharmacology , Resveratrol , Signal Transduction , Sirtuin 1 , Sirtuins/metabolism , Sterol Regulatory Element Binding Protein 1/biosynthesisABSTRACT
We report clinical, neuroradiologic features, and neuropathologic findings of a 76-year-old man with coexistent Pick's disease and progressive supranuclear palsy. The patient presented with loss of recent memory, abnormal behavior and change in personality at the age of 60. The symptoms were progressive. Three years later, repetitive or compulsive behavior became prominent. About 9 years after onset, he had difficulty moving and became bedridden because of a fracture of his left leg. His condition gradually deteriorated and he developed mutism and became vegetative. The patient died from pneumonia 16 years after the onset of symptoms. Serial MRI scans showed progressive cortex atrophy, especially in the bilateral frontal and temporal lobes. Macroscopic inspection showed severe atrophy of the whole brain, including cerebrum, brainstem and cerebellum. Microscopic observations showed extensive superficial spongiosis and severe neuronal loss with gliosis in the second and third cortical layers in the frontal, temporal and parietal cortex. There were Pick cells and argyrophilic Pick bodies, which were tau- and ubiquitin-positive in neurons of layers II-III of the above-mentioned cortex. Numerous argyrophilic Pick bodies were observed in the hippocampus, especially in the dentate fascia. In addition, moderate to severe loss of neurons was found with gliosis and a lot of Gallyas/tau-positive globus neurofibrillary tangles in the caudate nucleus, globus pallidus, thalamus, substantia nigra, locus coeruleus and dentate nucleus. Numerous thorned-astrocytes and coiled bodies but no-tuft shaped astrocytes were noted in the basal ganglion, brainstem and cerebellar white matter. In conclusion, these histopathological features were compatible with classical Pick's disease and coexistence with progressive supranuclear palsy without tuft-shaped astrocytes.
