ABSTRACT
Loss of autophagy is suggested to play a key role in the progression of osteoarthritis (OA). P63 is a member of the P53 family, which is widely dysregulated in various tumors. However, the specific role of P63 in chondrocyte autophagy has never been fully understood. Here, the expression level of P63 in the articular cartilages of OA patients and chondrocytes treated with 3-MA was explored using western blot. Autophagy was determined using transmission electron microscopy and mRFP-GFP-LC3 assay. Fewer autophagic vesicles were identified in the articular cartilages of OA patients compared with that of normal control. Both the mRNA and protein levels of P63 was markedly increased in the articular cartilages of OA patients compared with that of normal control. MTT assay demonstrated that P63 overexpression markedly reduced chondrocyte viability at 24, 36 and 48 h, while inhibition of P63 inhibited cell viability at 24, 36 and 48 h, respectively. Furthermore, autophagic flux assay showed that transfection of ad-P63 markedly decreased the yellow dots in chondrocytes, while inhibition of P63 induced chondrycyte autophagy. In summary, we first demonstrated that upregulation of P63 in the cartilage tissues of OA patients inhibited chondrocyte autophagy thereby contributing to the malignant progression of OA.
Subject(s)
Autophagy/genetics , Chondrocytes/pathology , Osteoarthritis/pathology , Transcription Factors/genetics , Tumor Suppressor Proteins/genetics , Adenine/analogs & derivatives , Adenine/pharmacology , Aged , Blotting, Western , Cartilage, Articular/pathology , Case-Control Studies , Disease Progression , Female , Humans , Male , Microscopy, Electron, Transmission , Middle Aged , Osteoarthritis/genetics , Time Factors , Transfection , Up-RegulationABSTRACT
Bryant traction is a commonly used method for femoral shaft fractures in children, but many disadvantages have been reported. Pavlik harness with exact clinical effect and fewer complications has gained increasing popularity in resent years. The objective of this study was to evaluate and compare modified Pavlik harness with Bryant traction for infant with a femoral shaft fracture. A retrospective study was performed of 38 infants treated with either modified Pavlik Harness or Bryant traction. All fractures were closed, isolated, and diaphyseal. We analyzed operative and radiographic data, complications, hospital charges, and functional outcome. Twenty-one patients, with a mean age of 5.9 months, were treated with modified Pavlik harnesses. Seventeen infants, with a mean age of 6.3 months, were treated with Bryant tractions. All fractures united within 3-5 weeks. The two cohorts were similar with respect to age, weight, and fracture union time. Four of the seventeen children treated with Bryant tractions had a skin complication that needed second intervention. No similar complications occurred in the modified Pavlik group (p = 0.03). There was a significant difference in hospital stay (modified Pavlik harness 1.4 days versus Bryant traction 17.8 days) and hospital charge (modified Pavlik harness 3209 Yuan versus Bryant traction 3759 Yuan) (p < 0.001). At one year visit, no difference existed between the two groups for standard clinical/functional criteria. There were no malunion, nonunion, or rotational deformities. Nor were there any significant limb length discrepancies, residual angular deformities.
Subject(s)
Femoral Fractures/therapy , Orthotic Devices , Traction , Child, Preschool , Female , Humans , Infant , Male , Retrospective Studies , Treatment OutcomeABSTRACT
A method of determining the contents of K, Na, Ca, Mg, P, Zn, Al, Ba, Co, Cu, Ni, Sr, Cr and Ti, fourteen elements, in urine of Xinjiang Kuitun fluorine poisoning and arsenic-fluoride poisoning patients was developed. The operation conditions of ICP-AES, and the lowest test concentration, precision and linear ranges were studied. The relative standard deviation of the method was 0.24%-2.47% (n=10), the average recoveries were 90.4%-00.5%. The contents of K and Na in urine of fluorine poisoning and arsenic-fluoride poisoning patients were higher than those of healthy contrast group. The contents of Ba, Co, Cu, Ni and Cr in the urine of arsenic-fluoride poisoning patients were higher than those of fluorine poisoning patients and healthy contrast group (P < 0.05). The contents of P, Ca, Mg, Zn, Al, Sr and Ti do not have statistic significance (P > 0.05). The method was sensitive, simple and accurate. The experiment data was reliable.
