ABSTRACT
BACKGROUND: Retroperitoneal leiomyosarcoma is a type of carcinoma with low incidence and poor prognosis, and prognostic factors are currently unknown. Therefore, our study aimed to investigate the predictive factors of RPLMS and establish prognostic nomograms. METHODS: Patients diagnosed with RPLMS between 2004 and 2017 were selected from the Surveillance, Epidemiology, and End Results (SEER) database. Prognostic factors were identified by univariate and multivariate COX regression analyses and used to generate nomograms to predict overall survival (OS) and cancer-specific survival (CSS). RESULTS: 646 eligible patients were randomly divided into training set (n = 323) and validation set (n = 323). Multivariate COX regression analysis indicated that the independent risk factors for OS and CSS were age, tumor size, grade, SEER stage, and surgery. In the nomogram of OS, the concordance indices (C-index) of the training and validation sets were 0.72 and 0.691, and in the nomogram of CSS, the C-indices of the training and validation sets were 0.737 and 0.737. Furthermore, calibration plots showed that the predicted results of the nomograms in the training and validation sets agree well with the actual observations. CONCLUSION: Age, tumor size, grade, SEER stage, and surgery were independent prognostic factors for RPLMS. The nomograms developed and validated in this study can accurately predict the OS and CSS of patients, which could help clinicians make individualized survival predictions. Finally, we make the two nomograms into two web calculators for the convenience of clinicians.
Subject(s)
Leiomyosarcoma , Retroperitoneal Neoplasms , Humans , Nomograms , Neoplasm Staging , SEER Program , Prognosis , InternetABSTRACT
The biological transformation of the bioactive dihydroisocoumarin, (-)-mellein, isolated from the marine-derived fungus Cladosporium sp., was studied. The preparative-scale culture of (-)-mellein with a marine isolate of a bacterium Stappia sp. resulted in the isolation of its oxidized metabolite, (3R,4S)-4-hydroxymellein. The stereostructure of the metabolite obtained was assigned on the basis of detailed physicochemical data analyses.
Subject(s)
Cladosporium/metabolism , Ochratoxins/metabolism , Rhodobacteraceae/isolation & purification , Rhodobacteraceae/metabolism , Seawater/microbiology , Biotransformation , Ochratoxins/chemistry , Rhodobacteraceae/chemistry , Rhodobacteraceae/genetics , StereoisomerismABSTRACT
Geraniol (1) is the biogenetic precursor of a number of monoterpenes. We tested various marine-derived microorganisms to determine their ability to biotransform 1. Only Hypocrea sp. was capable of transforming 1 into its oxidized derivative, 1,7-dihydroxy-3,7-dimethyl-(E)-oct- 2-ene (2). The structure of the metabolite obtained was assigned on the basis of detailed spectroscopic data analyses.
