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1.
Sensors (Basel) ; 24(10)2024 May 07.
Article in English | MEDLINE | ID: mdl-38793823

ABSTRACT

In the sixth generation (6G) era, intelligent machine network (IMN) applications, such as intelligent transportation, require collaborative machines with communication, sensing, and computation (CSC) capabilities. This article proposes an integrated communication, sensing, and computation (ICSAC) framework for 6G to achieve the reciprocity among CSC functions to enhance the reliability and latency of communication, accuracy and timeliness of sensing information acquisition, and privacy and security of computing to realize the IMN applications. Specifically, the sensing and communication functions can merge into unified platforms using the same transmit signals, and the acquired real-time sensing information can be exploited as prior information for intelligent algorithms to enhance the performance of communication networks. This is called the computing-empowered integrated sensing and communications (ISAC) reciprocity. Such reciprocity can further improve the performance of distributed computation with the assistance of networked sensing capability, which is named the sensing-empowered integrated communications and computation (ICAC) reciprocity. The above ISAC and ICAC reciprocities can enhance each other iteratively and finally lead to the ICSAC reciprocity. To achieve these reciprocities, we explore the potential enabling technologies for the ICSAC framework. Finally, we present the evaluation results of crucial enabling technologies to show the feasibility of the ICSAC framework.

2.
Article in English | MEDLINE | ID: mdl-38652631

ABSTRACT

Textbook question answering (TQA) task aims to infer answers for given questions from a multimodal context, including text and diagrams. The existing studies have aggregated intramodal semantics extracted from a single modality but have yet to capture the intermodal semantics between different modalities. A major challenge in learning intermodal semantics is maintaining lossless intramodal semantics while bridging the gap of semantics caused by heterogeneity. In this article, we propose an intermodal relation-aware heterogeneous graph network (IMR-HGN) to extract the intermodal semantics for TQA, which aggregates different modalities while learning features rather than representing them independently. First, we design a multidomain consistent representation (MDCR) to eliminate semantic gaps by capturing intermodal features while maintaining lossless intramodal semantics in multidomains. Furthermore, we present neighbor-based relation inpainting (NRI) to reduce semantic ambiguity via repairing fuzzy relations with correlations of relations. Finally, we propose hierarchical multisemantics aggregation (HMSA) to guarantee the completeness of semantics by aggregating features of nodes and relations with a reconstruction network (RN). Experimental results show that IMR-HGN could extract the intermodal semantics of answers, achieving a 2.16% improvement on the validation set of the TQA dataset and a 3.04% increase on the test set of the AI2D dataset.

3.
IEEE Trans Image Process ; 33: 2104-2115, 2024.
Article in English | MEDLINE | ID: mdl-38470577

ABSTRACT

Multi-scale detection based on Feature Pyramid Networks (FPN) has been a popular approach in object detection to improve accuracy. However, using multi-layer features in the decoder of FPN methods entails performing many convolution operations on high-resolution feature maps, which consumes significant computational resources. In this paper, we propose a novel perspective for FPN in which we directly use fused single-layer features for regression and classification. Our proposed model, You Only Look One Hourglass (YOLOH), fuses multiple feature maps into one feature map in the encoder. We then use dense connections and dilated residual blocks to expand the receptive field of the fused feature map. This output not only contains information from all the feature maps, but also has a multi-scale receptive field for detection. The experimental results on the COCO dataset demonstrate that YOLOH achieves higher accuracy and better run-time performance than established detector baselines, for instance, it achieves an average precision (AP) of 50.2 on a standard 3× training schedule and achieves 40.3 AP at a speed of 32 FPS on the ResNet-50 model. We anticipate that YOLOH can serve as a reference for researchers to design real-time detection in future studies. Our code is available at https://github.com/wsb853529465/YOLOH-main.

4.
Pest Manag Sci ; 80(2): 734-743, 2024 Feb.
Article in English | MEDLINE | ID: mdl-37779103

ABSTRACT

BACKGROUND: Rodent infestation is a global problem. Rodents cause huge harm to agriculture, forestry, and animal husbandry around the world and spread various zoonoses. In this study, we simulated the potentially suitable habitats of Bandicota indica and predicted the impact of future climate change on its distribution under different socio-economic pathway scenarios of CMIP6 using a parameter-optimized maximum entropy (MaxEnt) model. RESULTS: The average area under the receiver operating characteristic curve (AUC) value (0.958 ± 0.006) after ten repetitions proved the high accuracy of the MaxEnt model. Model results show that the annual mean temperature (≥ 15.93 °C), isothermality (28.52-80.49%), annual precipitation (780.13-3863.13 mm), precipitation of the warmest quarter (≥ 204.37 mm), and nighttime light (≥ 3.38) were important limiting environmental variables for the distribution of B. indica. Under current climate conditions, the projected potential suitable habitats for B. indica were mainly in India, China, Myanmar, Thailand, and Vietnam, which cover a total area of 301.70 × 104 km2 . The potentially suitable areas of B. indica in the world will expand under different future climate change scenarios by 1.61-17.65%. CONCLUSIONS: These results validate the potential influence of climate change on the distribution of B. indica and aid in understanding the linkages between B. indica niches and the relevant environment, thereby identifying urgent management areas where interventions may be necessary to develop feasible early warning and prevention strategies to protect against this rodent's spread. © 2023 Society of Chemical Industry.


Subject(s)
Climate Change , Murinae , Animals , Ecosystem , Agriculture , China
5.
Sensors (Basel) ; 23(5)2023 Feb 23.
Article in English | MEDLINE | ID: mdl-36904680

ABSTRACT

Specific emitter identification (SEI) and automatic modulation classification (AMC) are generally two separate tasks in the field of radio monitoring. Both tasks have similarities in terms of their application scenarios, signal modeling, feature engineering, and classifier design. It is feasible and promising to integrate these two tasks, with the benefit of reducing the overall computational complexity and improving the classification accuracy of each task. In this paper, we propose a dual-task neural network named AMSCN that simultaneously classifies the modulation and the transmitter of the received signal. In the AMSCN, we first use a combination of DenseNet and Transformer as the backbone network to extract the distinguishable features; then, we design a mask-based dual-head classifier (MDHC) to reinforce the joint learning of the two tasks. To train the AMSCN, a multitask cross-entropy loss is proposed, which is the sum of the cross-entropy loss of the AMC and the cross-entropy loss of the SEI. Experimental results show that our method achieves performance gains for the SEI task with the aid of additional information from the AMC task. Compared with the traditional single-task model, our classification accuracy of the AMC is generally consistent with the state-of-the-art performance, while the classification accuracy of the SEI is improved from 52.2% to 54.7%, which demonstrates the effectiveness of the AMSCN.

6.
Genome Biol ; 23(1): 203, 2022 09 26.
Article in English | MEDLINE | ID: mdl-36163035

ABSTRACT

BACKGROUND: The laboratory mouse was domesticated from the wild house mouse. Understanding the genetics underlying domestication in laboratory mice, especially in the widely used classical inbred mice, is vital for studies using mouse models. However, the genetic mechanism of laboratory mouse domestication remains unknown due to lack of adequate genomic sequences of wild mice. RESULTS: We analyze the genetic relationships by whole-genome resequencing of 36 wild mice and 36 inbred strains. All classical inbred mice cluster together distinctly from wild and wild-derived inbred mice. Using nucleotide diversity analysis, Fst, and XP-CLR, we identify 339 positively selected genes that are closely associated with nervous system function. Approximately one third of these positively selected genes are highly expressed in brain tissues, and genetic mouse models of 125 genes in the positively selected genes exhibit abnormal behavioral or nervous system phenotypes. These positively selected genes show a higher ratio of differential expression between wild and classical inbred mice compared with all genes, especially in the hippocampus and frontal lobe. Using a mutant mouse model, we find that the SNP rs27900929 (T>C) in gene Astn2 significantly reduces the tameness of mice and modifies the ratio of the two Astn2 (a/b) isoforms. CONCLUSION: Our study indicates that classical inbred mice experienced high selection pressure during domestication under laboratory conditions. The analysis shows the positively selected genes are closely associated with behavior and the nervous system in mice. Tameness may be related to the Astn2 mutation and regulated by the ratio of the two Astn2 (a/b) isoforms.


Subject(s)
Domestication , Genome , Animals , Mice , Nucleotides , Phenotype , Selection, Genetic , Whole Genome Sequencing
7.
Genes (Basel) ; 13(6)2022 06 17.
Article in English | MEDLINE | ID: mdl-35741841

ABSTRACT

Stropharia rugosoannulata uses straw as a growth substrate during artificial cultivation and has been widely promoted in China. However, its fruiting body formation and development processes have not been elucidated. In this study, the developmental transcriptomes were analyzed at three stages: the mycelium (G-S), primordium (P-S) and fruiting body (M-F) stages. A total of 9690 differentially expressed genes (DEGs) were identified in the different developmental stages. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses showed that these DEGs were involved mainly in hydrolase activity, structural molecule activity and oxidoreductase activity as well as xenobiotic biodegradation and metabolism and energy metabolism pathways. We further found that the higher expression of most carbohydrate enzyme (i.e., GH, CE, CBM, AA and PL) genes in the hyphal (i.e., G-S) stage was related mainly to substrate degradation, while the upregulation of glycosyltransferase (GT) gene expression in the P-S and M-F stages may be related to cell wall synthesis. In addition, we found that CO2-sensing-related genes (i.e., CA-2, CA-3, PKA-1 and PKA-2) were upregulated in the P-S and M-F stages, heat shock protein genes (HSP60 and HSP90) were significantly downregulated in the P-S stage and upregulated in the M-F stage and the transcription factors (i.e., steA, MYB, nosA, HAP1, and GATA-4/5/6) involved in growth and development were significantly upregulated in the P-S stage. These results suggest that environmental factors (i.e., CO2 and temperature) and transcription factors may play a key role in primordium formation. In short, this study provides new insights into the study of stimulating primordia formation affecting the development of fruiting bodies of S. rugosoannulata.


Subject(s)
Fruiting Bodies, Fungal , Transcriptome , Agaricales , Carbon Dioxide/metabolism , Fruiting Bodies, Fungal/genetics , Mycelium , Transcription Factors/genetics
8.
Sensors (Basel) ; 22(7)2022 Mar 25.
Article in English | MEDLINE | ID: mdl-35408157

ABSTRACT

With autonomous driving developing in a booming stage, accurate object detection in complex scenarios attract wide attention to ensure the safety of autonomous driving. Millimeter wave (mmWave) radar and vision fusion is a mainstream solution for accurate obstacle detection. This article presents a detailed survey on mmWave radar and vision fusion based obstacle detection methods. First, we introduce the tasks, evaluation criteria, and datasets of object detection for autonomous driving. The process of mmWave radar and vision fusion is then divided into three parts: sensor deployment, sensor calibration, and sensor fusion, which are reviewed comprehensively. Specifically, we classify the fusion methods into data level, decision level, and feature level fusion methods. In addition, we introduce three-dimensional(3D) object detection, the fusion of lidar and vision in autonomous driving and multimodal information fusion, which are promising for the future. Finally, we summarize this article.


Subject(s)
Automobile Driving , Radar , Algorithms , Calibration , Vision, Ocular
9.
Article in English | MEDLINE | ID: mdl-35235523

ABSTRACT

Heterogeneous information networks (HINs) are potent models of complex systems. In practice, many nodes in an HIN have their attributes unspecified, resulting in significant performance degradation for supervised and unsupervised representation learning. We developed an unsupervised heterogeneous graph contrastive learning approach for analyzing HINs with missing attributes (HGCA). HGCA adopts a contrastive learning strategy to unify attribute completion and representation learning in an unsupervised heterogeneous framework. To deal with a large number of missing attributes and the absence of labels in unsupervised scenarios, we proposed an augmented network to capture the semantic relations between nodes and attributes to achieve a fine-grained attribute completion. Extensive experiments on three large real-world HINs demonstrated the superiority of HGCA over several state-of-the-art methods. The results also showed that the complemented attributes by HGCA can improve the performance of existing HIN models.

10.
Sci Rep ; 11(1): 19005, 2021 09 24.
Article in English | MEDLINE | ID: mdl-34561500

ABSTRACT

Influenced by climate change and human activities, especially the completion and operation of cascade reservoirs in the middle and lower reaches of Jinsha River since 2012, new changes have taken place in the water and sediment characteristics of the Three Gorges Reservoir (TGR) in recent years. In this paper, a one-dimensional unsteady water and sediment mathematical model of the main and tributary rivers of the TGR is established, and the main calculation parameters of the model are calibrated with the measured water and sediment data from January 1, 2008 to December 31, 2017. In view of the different combinations of inflow water and sediment that may occur in the TGR under the condition of new water and sediment, the long-term changes of sediment erosion and deposition and the balance of reservoir deposition in the TGR are studied using the model. The results show that: (1) Under the new conditions of water and sediment, the amount of sediment in the TGR accounts for only 14.8% and 35.8% of that in 1956-1990 and 2003-2012, respectively; (2) The variation process of water level, discharge and sediment concentration of each station along the route calculated by the model is basically consistent with the measured results, and the calculated values of total deposition amount and deposition distribution are also basically consistent with the measured results. The verification results of the model are in accordance with the measured values; (3) Under the water-sediment conditions during 1961-1970 and 1991-2000, the model predicted the estimates of 320 and 430 years for the TGR to reach a sedimentation balance, respectively. Under the new water-sediment conditions, it takes 560 years at most and 450 years at least to reach the sedimentation balance for the TGR, and the corresponding condition is the typical year with less water-less sediment and more water-more sediment, respectively. The research results of this paper can provide a new reference for the long-term safe operation and operation optimization of the TGR.

11.
Front Cardiovasc Med ; 8: 691609, 2021.
Article in English | MEDLINE | ID: mdl-34355029

ABSTRACT

Objectives: To evaluate the effect of in-hospital pulmonary rehabilitation (PR) on short-term pulmonary functional recovery in patients with COVID-19. Methods: Patients with COVID-19 (n = 123) were divided into two groups (PR group or Control group) according to recipient of pulmonary rehabilitation. Six-min walk distance (6MW), heart rate (HR), forced vital capacity (FVC), forced expiratory volume in 1 s (FEV1), diffusing capacity of the lung for carbon monoxide (DLCO), and CT scanning were measured at the time of discharge, 1, 4, 12, and 24 weeks. Results: At week one, both PR group and Control group showed no significant changes in pulmonary function. At 4 and 12 weeks, 6MW, HR, FVC, FEV1, and DLCO improved significantly in both groups. However, the improvement in the PR group was greater than the Control group. Pulmonary function in the PR group returned to normal at 4 weeks [FVC (% predicted, PR vs. Control): 86.27 ± 9.14 vs. 78.87 ± 7.55; FEV1 (% predicted, PR vs. Control) 88.76 ± 6.22 vs. 78.96 ± 6.91; DLCO (% predicted, PR vs. Control): 87.27 ± 6.20 vs. 77.78 ± 5.85] compared to 12 weeks in the control group [FVC (% predicted, PR vs. Control): 90.61 ± 6.05 vs. 89.96 ± 4.05; FEV1 (% predicted, PR vs. Control) 94.06 ± 0.43 vs. 93.85 ± 5.61; DLCO (% predicted, PR vs. Control): 91.99 ± 8.73 vs. 88.57 ± 5.37]. Residual lesions on CT disappeared at week 4 in 49 patients in PR group and in 28 patients in control group (p = 0.0004). Conclusion: Pulmonary rehabilitation could accelerate the recovery of pulmonary function in patients with COVID-19.

12.
Sensors (Basel) ; 21(12)2021 Jun 08.
Article in English | MEDLINE | ID: mdl-34201383

ABSTRACT

Due to the increasing number of vehicles equipped with millimeter wave (mmWave) radars, interference among automotive radars is becoming a major issue. This paper explores the automotive radar interference in both two-lane and multi-lane scenarios using stochastic geometry. We derive closed-form expressions for mean and variance of interference power considering directional antenna with constant and Gaussian decaying gains. In view of the sensitivity of mmWave radar signals to the blockages, we propose a blockage model including partially and completely blocking, and then calculate the effective number of the interferers. By means of modeling randomness for interferers and blockages as Poisson point process, we characterize the statistics of radar interference under different conditions. We further utilize the interference characterization to estimate the successful ranging probability of automotive radars. These theoretical analyses are verified by using Monte Carlo simulations. The results show that the increasing interfering density and ranging distance largely degrade the radar detection performance, whereas the interference levels decrease as blockage intensity increases.

13.
Front Neurorobot ; 15: 652562, 2021.
Article in English | MEDLINE | ID: mdl-33935676

ABSTRACT

A number of methods have been proposed for face reconstruction from single/multiple image(s). However, it is still a challenge to do reconstruction for limited number of wild images, in which there exists complex different imaging conditions, various face appearance, and limited number of high-quality images. And most current mesh model based methods cannot generate high-quality face model because of the local mapping deviation in geometric optics and distortion error brought by discrete differential operation. In this paper, accurate geometrical consistency modeling on B-spline parameter domain is proposed to reconstruct high-quality face surface from the various images. The modeling is completely consistent with the law of geometric optics, and B-spline reduces the distortion during surface deformation. In our method, 0th- and 1st-order consistency of stereo are formulated based on low-rank texture structures and local normals, respectively, to approach the pinpoint geometric modeling for face reconstruction. A practical solution combining the two consistency as well as an iterative algorithm is proposed to optimize high-detailed B-spline face effectively. Extensive empirical evaluations on synthetic data and unconstrained data are conducted, and the experimental results demonstrate the effectiveness of our method on challenging scenario, e.g., limited number of images with different head poses, illuminations, and expressions.

14.
Appl Microbiol Biotechnol ; 105(7): 2815-2829, 2021 Apr.
Article in English | MEDLINE | ID: mdl-33675375

ABSTRACT

Zn(II)2Cys6 transcription factors are critical for the reproductive growth and sexual development of fungi, but their roles in Basidiomycota remain unclear. In this study, the Hypsizygus marmoreus gene hada-1 was shown to encode a Zn(II)2Cys6 transcription factor, the growth rate of mycelia was decreased, hyphae were angulated, and fruiting body development was hindered in the hada-1-silenced strains. In addition, mitochondrial stability was lost, and the mitochondria morphologies changed from oval shaped to dumbbell or linear shaped in the silenced strains. Regarding mitochondrial instability, the mitochondrial complex II, III, and V activities and adenosine triphosphate content were significantly decreased. At the same time, the activities of the carbohydrate metabolism-related enzymes glucose-6-plosphatase, glucose dehydrogenase, and laccase were significantly decreased, which might have resulted in the reduction of carbon metabolism. Furthermore, hada-1 was shown to regulate the reactive oxygen species (ROS) level; compared with the wild-type (WT) strain, the silenced mycelia exhibited higher ROS contents and were more sensitive to oxidative stress. Taken together, these results indicate that, as a global regulator, hada-1 plays crucial roles in mycelial growth, fruiting body development, carbon metabolism, mitochondrial stability, and oxidative stress in the basidiomycete H. marmoreus. KEY POINTS: • Zn(II)2Cys6 transcription factor, mitochondrial stability, fruiting body development.


Subject(s)
Agaricales , Mycelium/genetics , Transcription Factors , Zinc
15.
Appl Microbiol Biotechnol ; 104(24): 10555-10570, 2020 Dec.
Article in English | MEDLINE | ID: mdl-33175244

ABSTRACT

Glutathione peroxidase (GPX) is one of the most important antioxidant enzymes for maintaining reactive oxygen species (ROS) homeostasis. Although studies on fungi have suggested many important physiological functions of GPX, few studies have examined the role of this enzyme in Basidiomycetes, particularly its functions in fruiting body developmental processes. In the present study, GPX-silenced (GPxi) strains were obtained by using RNA interference. The GPxi strains of Hypsizygus marmoreus showed defects in mycelial growth and fruiting body development. In addition, the results indicated essential roles of GPX in controlling ROS homeostasis by regulating intracellular H2O2 levels, maintaining GSH/GSSG balance, and promoting antioxidant enzyme activity. Furthermore, lignocellulose enzyme activity levels were reduced and the mitochondrial phenotype and mitochondrial complex activity levels were changed in the H. marmoreus GPxi strains, possibly in response to impediments to mycelial growth and fruiting body development. These findings indicate that ROS homeostasis has a complex influence on growth, fruiting body development, GSH/GSSG balance, and carbon metabolism in H. marmoreus.Key points• ROS balance, energy metabolism, fruiting development.


Subject(s)
Glutathione , Hydrogen Peroxide , Agaricales , Glutathione Peroxidase/genetics , Homeostasis , Reactive Oxygen Species
16.
Fungal Biol ; 124(6): 551-561, 2020 06.
Article in English | MEDLINE | ID: mdl-32448446

ABSTRACT

Hypsizygus marmoreus is an important commercial edible fungus, but the lack of basic studies on this fungus has hindered further development of its commercial value. In this study, we found that the treatment of damaged vegetative mycelia with 1 mM l-ascorbic acid (ASA) significantly increased the antioxidant enzyme activities (GPX, GR, CAT and SOD) and antioxidant contents (GSH and ASA) and reduced the ROS levels (H2O2 and O2-) in mechanically damaged mycelia. Additionally, this treatment increased mycelial biomass. At the reproductive stage, our results demonstrated that the treatment of damaged H. marmoreus mycelia with 2.24 mM ASA significantly increased the antioxidant enzyme activities (GPX, GR, GST, TRXR and CAT), endogenous ASA contents and GSH/GSSG ratios in different developmental stages and significantly decreased the MDA and H2O2 contents. Furthermore, this study showed that the expression levels of the antioxidant enzyme genes were consistent with the enzyme activities. Damaged mycelia treated with ASA regenerated 2-3 d earlier than the control group and showed significantly enhanced fruiting body production. These results suggested that exogenous ASA regulated mycelia intracellular ASA content to increase mycelial antioxidant abilities, induce the regeneration of damaged mycelia and regulate the development of fruiting bodies in H. marmoreus.


Subject(s)
Agaricales/drug effects , Agaricales/physiology , Antioxidants/metabolism , Ascorbic Acid/pharmacology , Fruiting Bodies, Fungal/growth & development , Mycelium/physiology , Agaricales/growth & development , Mycelium/drug effects , Oxidative Stress/drug effects , Oxidoreductases/genetics , Oxidoreductases/metabolism , Reactive Oxygen Species/metabolism , Regeneration
17.
Sensors (Basel) ; 20(4)2020 Feb 11.
Article in English | MEDLINE | ID: mdl-32053909

ABSTRACT

For autonomous driving, it is important to detect obstacles in all scales accurately for safety consideration. In this paper, we propose a new spatial attention fusion (SAF) method for obstacle detection using mmWave radar and vision sensor, where the sparsity of radar points are considered in the proposed SAF. The proposed fusion method can be embedded in the feature-extraction stage, which leverages the features of mmWave radar and vision sensor effectively. Based on the SAF, an attention weight matrix is generated to fuse the vision features, which is different from the concatenation fusion and element-wise add fusion. Moreover, the proposed SAF can be trained by an end-to-end manner incorporated with the recent deep learning object detection framework. In addition, we build a generation model, which converts radar points to radar images for neural network training. Numerical results suggest that the newly developed fusion method achieves superior performance in public benchmarking. In addition, the source code will be released in the GitHub.

18.
Heliyon ; 5(9): e02458, 2019 Sep.
Article in English | MEDLINE | ID: mdl-31687559

ABSTRACT

Sediment accumulation has been the most important factor influencing the comprehensive benefit of reservoirs. A quantitative investigation of the sediment trapping efficiencies of reservoirs is a key to understanding the impact of sedimentation on reservoirs. Generally, the simplest method to assess sediment accumulation ratio is adopting sediment concentration curve with trap efficiency (TE) of the reservoir. Many empirical and semi-empirical models have been proposed to determine this term related to the average annual inflow, features and characteristics of the reservoir watershed area. In this article four different empirical models decided by capacity to inflow ratio (C/I), capacity to watershed ratio (C/W) were used. These different models were summarized and utilized to determine TE of large reservoirs on the Upper Yangtze River for recent decades. Based on these conventional models, an improved model to estimate sediment trapping efficiency is proposed, and experimental data from other 18 reservoirs come from different basins were used to validate the model. The results indicate that the sediment trapping efficiencies that were estimated by the four empirical model were similar to the measured efficiencies for reservoirs on the Upper Yangtze River. Among the the Brune and Siyam empirical models were the most reliable and can be applied to estimate sediment trapping efficiency for reservoirs on the Upper Yangtze River. The improved model takes the capacity/annual inflow ratio and capacity/watershed area ratio into account comprehensively, the effect of particle size and settling velocity of the sediment are also considered, it is more applicable and accuracy to predict large reservoir sediment trapping efficiency. The results of this study provide a valuable reference for predicting large reservoir sedimentation and sediment regulation.

19.
AMB Express ; 9(1): 103, 2019 Jul 12.
Article in English | MEDLINE | ID: mdl-31300949

ABSTRACT

Morchella importuna has been artificially cultivated, but stable production remains difficult because its mechanisms of fruiting body formation are unclear. To investigate the fruiting body formation mechanisms, we sequenced the transcriptomes of Morchella importuna at the mycelial and young fruiting body stages. Among the 12,561 differentially expressed genes (DEGs), 9215 were upregulated, and 3346 were downregulated. DEG enrichment analysis showed that these genes were enriched in the "generation of precursor metabolites and energy", "carbohydrate catabolic process", and "oxidoreductase activity" Gene Ontology (GO) functional categories. Enzyme activity assay results indicated that the activity levels of CAZymes (carbohydrate-active enzymes), oxidoreductases (SOD (superoxide dismutase), CAT (catalase)) and mitochondrial complex (complex I, II, III) proteins were significantly increased from the mycelial stage to the young fruiting body stage. In addition, the genes encoding CAZymes, mitochondrial proteins, oxidoreductases and heat shock proteins had higher expression levels in the young fruiting body stage than in the mycelial stage, and the qRT-PCR results showed similar trends to the RNA-Seq results. In summary, these results suggest that carbohydrate catabolism and energy metabolism are significantly enhanced in the young fruiting body stage and that growth environment temperature changes affect the formation of fruiting bodies.

20.
Sensors (Basel) ; 19(8)2019 Apr 18.
Article in English | MEDLINE | ID: mdl-31003484

ABSTRACT

Offline-trained Siamese networks are not robust to the environmental complication in visual object tracking. Without online learning, the Siamese network cannot learn from instance domain knowledge and adapt to appearance changes of targets. In this paper, a new lightweight Siamese network is proposed for feature extraction. To cope with the dynamics of targets and backgrounds, the weight in the proposed Siamese network is updated in an online manner during the tracking process. In order to enhance the discrimination capability, the cross-entropy loss is integrated into the contrastive loss. Inspired by the face verification algorithm DeepID2, the Bayesian verification model is applied for candidate selection. In general, visual object tracking can benefit from face verification algorithms. Numerical results suggest that the newly developed algorithm achieves comparable performance in public benchmarks.

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