ABSTRACT
Major vascular injuries during laparoscopic cholecystectomy are rare, usually readily apparent, and immediately treated. We report a case of delayed presentation of a retroperitoneal vascular injury. The patient presented with abdominal pain and increasing edema of the lower extremities 1 year after laparoscopic cholecystectomy and was found to have an ilio-iliac arteriovenous fistula. Endovascular treatment was accomplished using a graft-covered polytetrafluoroethylene stent. The patient remained free of symptoms at 1-year follow-up.
Subject(s)
Arteriovenous Fistula/etiology , Arteriovenous Fistula/therapy , Cholecystectomy, Laparoscopic/adverse effects , Stents , Female , Humans , Iliac Artery/injuries , Iliac Vein/injuries , Middle Aged , PolytetrafluoroethyleneABSTRACT
BACKGROUND: Cytomegalovirus (CMV) is responsible for the most common opportunistic viral infection in patients with acquired immunodeficiency syndrome (AIDS). The colon is a common site for CMV infection in patients positive for the human immunodeficiency virus (HIV). The clinical diagnosis of CMV infection is based on the characteristic endoscopic appearance of extensive ulceration of the gastric mucosa. METHODS: A 54-year-old HIV-positive man visited our hospital because of high fever. The patient presented with diarrhea, and colonoscopy was performed. Ganciclovir was administered, with a good clinical response. However, the patient complained of the same symptoms again 6 months later. Nested polymerase chain reaction (PCR) was performed on all the patient's samples to detect CMV, followed by sequencing of the UL97 gene for CMV resistance detection. RESULTS: The PCR tests for Legionella, Chlamydia pneumoniae, Pneumocystis carinii, and Aspergillus were negative. DNA preparations from biopsy specimens of areas of colon ulceration were positive for CMV. Six months after treating the colon ulcerations, the PCR for CMV was positive, and the possible emergence of CMV mutants conferring ganciclovir resistance was examined. Direct sequencing of the PCR products revealed the known V594 mutation in the UL97 gene predisposing for ganciclovir resistance as well as the polymorphisms (579, GGC-->GGT) and (598, GGT-->GGC) in all samples tested. CONCLUSIONS: In summary, molecular biology methods can be used for early detection of CMV in characteristic colonic lesions in HIV-1-positive patients. Furthermore, detection of mutant strains resistant to antiviral drugs as well as polymorphisms elucidate the natural history of the infection.
Subject(s)
AIDS-Related Opportunistic Infections/diagnosis , Colonic Diseases/virology , Cytomegalovirus Infections/diagnosis , HIV Seropositivity , HIV-1/immunology , AIDS-Related Opportunistic Infections/drug therapy , Antiviral Agents/therapeutic use , Colonic Diseases/diagnosis , Colonic Diseases/drug therapy , Cytomegalovirus/genetics , Cytomegalovirus Infections/drug therapy , DNA, Viral/genetics , Drug Resistance, Viral , Ganciclovir/therapeutic use , Humans , Male , Middle Aged , Recurrence , Sequence Analysis, DNAABSTRACT
Over the past few decades, a great scientific effort has been made to treat gastroesophageal reflux disease (GERD). This reflects a trend in modem medicine toward optimizing quality of life, reducing health-related lost working hours, and minimizing costs of chronic treatments. It also reflects a revived interest in diseases that can be studied using novel equipment and that can be cured using minimally invasive techniques. In an effort to further minimize surgical trauma, novel endoscopic techniques are beginning to challenge the standard therapeutic approach to GERD.
