ABSTRACT
A method for preparing tissue sections for automatic image analysis of glycogen is described. Large semithin sections of epoxy embedded tissue fixed in glutaraldehyde-osmium were stained with Schiff reagent and acriflavine (fluorescent staining) after resin removal and periodic acid oxidation in ethanol. We found it essential to avoid tissue rehydration before final staining. The Schiff stain permits an assessment of the cellular volume of glycogen, and the acriflavine allows a fluorometric evaluation of glycogen density.
Subject(s)
Acriflavine , Aminoacridines , Esophagus/analysis , Glycogen/analysis , Rosaniline Dyes , Sulfhydryl Compounds , Toluidines , Coloring Agents , Epithelium/analysis , Ethanol , Histocytochemistry , Humans , Oxidation-Reduction , Periodic Acid , Staining and LabelingABSTRACT
The authors present a histological and ultrastructural analysis of normal and parakeratotic human esophageal epithelium. Parakeratosis is encountered frequently during routine examinations of noncancerous esophageal mucosa in patients with carcinoma of the esophagus. Morphologically, this alteration corresponds to an incomplete keratinization of the esophageal epithelium with an excessive accumulation of keratin microfilaments, nuclear maturation reaching the stages of pyknosis and lysis, and anomalies in surface cell desquamation.
