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Epidemiol Infect ; 116(3): 323-9, 1996 Jun.
Article in English | MEDLINE | ID: mdl-8666077

ABSTRACT

In view of the known cross-reactivity of sera from patients with intestinal parasites to some Schistosoma mansoni antigens, field work was conducted in an area of Venezuela non-endemic for schistosomiasis using the routine immunoenzymatic assay (ELISA) with soluble egg antigen (SEA). False positive reactions represented 15.3% of the total population as determined by SEA-ELISA. SEA-immunoblotting of the false positive sera indicated that protein fractions of 91 and 80 kDa appear to be responsible for cross-reactivity. Sera from hookworm infected individuals produced a higher frequency and intensity of cross-reaction than other sera. SEA-fractions of 105, 54, 46, 42, 32, 25 and 15 kDa were the most specific.


Subject(s)
Hookworm Infections/epidemiology , Schistosomiasis mansoni/epidemiology , Adolescent , Adult , Animals , Antigens, Helminth/isolation & purification , Child , Child, Preschool , Cross Reactions , Enzyme-Linked Immunosorbent Assay , False Positive Reactions , Hookworm Infections/complications , Humans , Infant , Middle Aged , Prevalence , Reproducibility of Results , Schistosoma mansoni/isolation & purification , Schistosomiasis mansoni/complications , Seroepidemiologic Studies , Venezuela/epidemiology
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