ABSTRACT
OBJECTIVE: To determine the content of epidermal growth factor receptor (EGFR) using a radioligand method in breast cancer and to analyze the relationship between the EGFR levels and the characteristics of patients and tumors. Prognostic significance was also analyzed. MATERIAL AND METHODS: EGFR was measured by a single point radioligand assay in 265 invasive breast carcinomas tissues. In addition, estrogen and progesterone receptors (ER and PR) were measured by enzymatic immunoassays. We analyze the relationship of EGFR levels with the different clinico-pathologic parameters. RESULTS: EGFR levels in breast carcinomas varied widely (0.1 to 403) with a median at 4 fmol/mg prot. The significantly higher concentrations of EGFR were detected in patients under 60 years old (p = 0.042), undifferentiated tumors (p = 0.04), and carcinomas with negative ER and PR (p < 0.019 y p < 0018, respectively). In addition, there was a negative correlation between EGFR and the ER and PR levels (p < 0.05). EGFR levels did not show any relationship with the patient's prognosis. CONCLUSIONS: In addition, intratumoral levels of EGFR in breast carcinomas vary widely and the highest concentrations are associated with the most aggresive characteristics of the tumor.
Subject(s)
Biomarkers, Tumor/analysis , Breast Neoplasms/chemistry , Carcinoma/chemistry , ErbB Receptors/analysis , Adult , Aged , Aged, 80 and over , Breast Neoplasms/mortality , Breast Neoplasms/pathology , Carcinoma/mortality , Carcinoma/pathology , Female , Humans , Life Tables , Middle Aged , Neoplasm Invasiveness , Neoplasm Proteins/analysis , Prognosis , Radioligand Assay , Receptors, Estrogen/analysis , Receptors, Progesterone/analysis , Survival AnalysisABSTRACT
Objetivo: Cuantificar el contenido del receptor del factor de crecimiento epidérmico (EGFR) mediante técnica de radioligando en el cáncer de mama, y analizar su relación con las características de las pacientes y de sus tumores así como su significado pronóstico. Material y método: Se cuantificó la concentración de EGFR mediante técnica de radioligando de un sólo punto en 265 carcinomas invasivos de mama. Igualmente se determinaron los receptores de estrógenos (RE) y progesterona (RP) mediante inmunoensayo enzimático. Analizamos el contenido de EGFR y su relación con los diferentes parámetros clínico-patológicos. Resultados: Los niveles de EGFR en carcinomas de mama oscilaron ampliamente (de 0,1 a 403) con una mediana de 4 fmol/mg prot. Los niveles significativamente más altos de EGFR se detectaron en las pacientes menores de 60 años (p < 0,042), en los tumores indiferenciados (p < 0,004) y en los que eran negativos para RE y RP (p < 0,019 y p < 0,018, respectivamente). Además, hubo correlación negativa entre los niveles de EGFR y los de RE y RP (p < 0,05). No observamos relación entre los niveles de EGFR y el pronóstico de las pacientes. Conclusión: Los niveles intratumorales de EGFR en los carcinomas mamarios presentan una amplia variabilidad, y las concentraciones más elevadas se relacionan con las características más agresivas del tumor (AU)
Subject(s)
Middle Aged , Adult , Aged , Aged, 80 and over , Female , Humans , Biomarkers, Tumor , Life Tables , Survival Analysis , Neoplasm Invasiveness , Receptors, Progesterone , Receptors, Estrogen , ErbB Receptors , Prognosis , Carcinoma , Neoplasm Proteins , Radioligand Assay , Breast NeoplasmsABSTRACT
We made a prospective study of the intercritical changes in cerebral perfusion using SPECT with 99mTc-HMPAO in 33 adult patients with focal epilepsy which was resistant to polypharmacy and showed normal MR, to evaluate the relationship between these changes and the clinico-electrical focus (FCE) and the clinical features of epilepsy. All SPECT studies (100%) showed one or more areas of hypoperfusion. There was good topographical relationship between the perfusion defects and FCE which coincided exactly in 15 patients (45.4%); was acceptable with FCE identification but with associated defects in 12 (36.4%) and poor, bearing no relation to each other in 6 (18.2%). There was no correlation between a good, acceptable or poor relationship of SPECT-FCE and the age of the patient, time-course of the illness, number of crises, number of drugs or type of treatment given. There were more cases showing poor relationship amongst the pure temporal lobe foci (p < 0.05), but when these were considered together with the fronto-temporal cases, no difference was seen when compared with the extra-temporal cases. There was a tendency to greater secondary generalization of the crises in the group showing a good relationship. There were more cases of a clinical history of previous CNS diffuse lesions (anoxia, trauma or meningitis) amongst those with a poor relationship (83%) but not amongst those with a good relationship (20%, p < 0.05), or an acceptable relationship (25%, p < 0.05). This would seem to suggest that the anomalies found were more a result of the cause of the epilepsy than of the repetition of crises. SPECT should be included in the diagnostic algorithms of focal epilepsy with normal.
