ABSTRACT
The aim of this work was the simultaneous encapsulation of magnetic nanoparticles (MNPs) and zinc(II) phthalocyanine (ZnPc) in poly(methyl methacrylate) (PMMA) (MNPsZnPc-PMMA) nanoparticles (NPs) by miniemulsion polymerization and to evaluate the photobiological activity and/or hyperthermia (HPT) against human glioblastoma cells (U87MG). MNPsZnPc-PMMA NPs presented an average diameter of 104 ± 2.5 nm with a polydispersity index (PdI) of 0.14 ± 0.03 and negative surface charge - 47 ± 2.2 mV (pH 7.4 ± 0.1). The encapsulation efficiency (EE%) of ZnPc was 85.7% ± 1.30. The release of ZnPc from PMMA NPs was slow and sustained without the presence of burst effect, indicating a homogeneous distribution of the drug in the polymeric matrix. In the biological assay, MNPsZnPc-PMMA NPs showed considerable cytotoxic effect on U87MG cells only after activation with visible light at 675 nm (photodynamic therapy, PDT) or after application of an alternating magnetic field. The simultaneous encapsulation of MNPs and ZnPc in a drug delivery system with sustained release can be a new alternative for cancer treatment leading to significant tumor regression after minimum doses of heat dissipation and light.
Subject(s)
Indoles/chemistry , Nanoparticles/chemistry , Organometallic Compounds/chemistry , Polymethyl Methacrylate/chemistry , Antineoplastic Agents/administration & dosage , Cell Line, Tumor , Cell Survival/drug effects , Delayed-Action Preparations , Drug Compounding , Drug Delivery Systems , Emulsions , Humans , Isoindoles , Light , Magnetic Fields , Magnetite Nanoparticles , Nanoparticles/radiation effects , Polymerization , Zinc CompoundsABSTRACT
293T and Sk-Hep-1 cells were transduced with a replication-defective self-inactivating HIV-1 derived vector carrying FVIII cDNA. The genomic DNA was sequenced to reveal LTR/human genome junctions and integration sites. One hundred and thirty-two sequences matched human sequences, with an identity of at least 98%. The integration sites in 293T-FVIIIDB and in Sk-Hep-FVIIIDB cells were preferentially located in gene regions. The integrations in both cell lines were distant from the CpG islands and from the transcription start sites. A comparison between the two cell lines showed that the lentiviral-transduced DNA had the same preferred regions in the two different cell lines.
