ABSTRACT
ABSTRACT Dental trauma can happen at any stage of life; however, it is very common in preschool and school aged children. Dislocation injuries in the primary dentition are the most commonly described traumatic dental injuries in the literature. Tooth displacement in the primary dentition is favored due to the greater porosity and resilience of the alveolar bone. Through the study of a clinical case, this article presents the clinical conduct performed in the case of trauma of lateral dislocation with involvement of a fracture of the buccal bone plate in order to assist the clinical practice of dentists who care for children. The treatment carried out in this clinical case study took into account the magnitude of the displacement, the degree of development of the tooth and its relationship with the permanent germ, the length of time since the trauma, and the degree of cooperation of the child in the face of the suggested treatment. This treatment does not only involve emergency intervention; the patient must undergo a radiographic clinical follow-up and control after 30, 60 and 120 days, as well as annually, until the Exfoliation of the deciduous tooth. The child's health was restored aesthetically and functionally.
RESUMO Traumatismos dentários podem acontecer em qualquer fase da vida, entretanto, é muito comum em crianças na idade pré-escolar e escolar. As lesões de luxação na dentição decídua são as lesões dentárias traumáticas mais comumente descritas na literatura. O deslocamento dentário na dentição decídua é favorecido devido à maior porosidade e resiliência do osso alveolar. Através do relato de caso clínico, o artigo apresenta a conduta clínica realizada diante um trauma de luxação lateral com acometimento de fratura de tábua óssea vestibular no intuito de auxiliar a prática clínica de cirurgiões-dentistas que atendem crianças. O tratamento realizado neste caso clínico levou em consideração a magnitude do deslocamento, o grau de desenvolvimento do elemento dentário e sua relação com o germe do permanente, o tempo de procura após o trauma e o grau de cooperação da criança diante o tratamento sugerido. O tratamento não envolve apenas a intervenção de urgência, o paciente deve passar por um acompanhamento e controle clínico radiográfico depois de 30, 60 e 120 dias e anualmente até a Esfoliação do dente decíduo. Pôde-se verificar que foi restabelecida a saúde da criança no aspecto estético e funcional.
ABSTRACT
ABSTRACT Objective: The purpose of the present in vitro study was to evaluate the efficiency of three different instruments in the root canals of artificial primary molars as well as the quality of the fillings. Methods: Sixty artificial primary molars were divided into three groups of 20 teeth. Each group was submitted to a different technique: Group 1 - conventional manual technique using Kerr files; Group 2 - manual technique with nickel-titanium files; Group 3 - NiTi rotary technique. The root canals were filled with calcium hydroxide paste. Aspects such as homogeneity of the fillings, taper and flowability of the preparations were considered comparatively to assess the quality of the instrumentations. The non-parametric Kruskal-Wallis and Mann-Whitney tests were used to compare the efficiency of the preparation of the root canal considering the preparation times of them. Results: All three techniques were equally effective at shaping the root canals of primary teeth. The working time was shorter with the rotary instrument (p=0.000). In the pairwise comparisons, working time was shorter with the rotary instrument compared to the manual techniques with steel files (p=0.000) and NiTi files (p=0.000). Comparing the manual techniques, the working time was shorter with NiTi files compared to steel files (p=0.011). Moreover, less variability in the preparation time was found when the rotary instrument was used. Conclusions: The mechanized rotary method led to a shorter operating time in comparison to the manual techniques and is therefore a preferable option for the preparation of the root canals of primary teeth.
RESUMO Objetivo: Avaliar a eficiência de três instrumentos diferentes nos canais radiculares de molares decíduos artificiais, bem como a qualidade das obturações. Métodos: Sessenta molares decíduos artificiais foram divididos em três grupos de 20 dentes. Cada grupo foi submetido a uma técnica diferente: Grupo 1 - técnica manual convencional com limas Kerr; Grupo 2 - técnica manual com limas de níquel-titânio; Grupo 3 - Técnica rotatória de níquel-titânio. Os canais radiculares foram preenchidos com pasta de hidróxido de cálcio. Aspectos como homogeneidade das obturações, conicidade e fluidez das preparações foram considerados comparativamente para avaliar a qualidade das instrumentações. Os testes não paramétricos de Kruskal-Wallis e Mann-Whitney foram utilizados para comparar a eficiência do preparo do canal radicular considerando os tempos de preparo dos mesmos. Resultados: Todas as três técnicas foram igualmente eficazes na modelagem dos canais radiculares. O tempo de trabalho foi menor com o instrumento rotatório (p=0,000). Nas comparações pareadas, o tempo de trabalho foi menor com o instrumento rotatório em comparação com as técnicas manuais com limas de aço (p=0,000) e limas de NiTi (p=0,000). Comparando as técnicas manuais, o tempo de trabalho foi menor com as limas de NiTi em comparação com as limas de aço (p=0,011). Além disso, foi encontrada menor variabilidade no tempo de preparo quando o instrumento rotatório foi utilizado. Conclusão: O método rotatório levou um menor tempo operatório em comparação com as técnicas manuais e, portanto, é uma opção preferível para o preparo dos canais radiculares de dentes decíduos.
ABSTRACT
Abstract Objective: To perform an in vivo evaluation on the agreement between measurements of working length obtained by conventional radiographic examinations and an apex locator in deciduous teeth with or without root resorption. Material and Methods: Nine canals of teeth from children ranging from 3 to 5 years old were selected. Endodontic access was performed with a spherical diamond tip, the pulp was removed with Kerr-type steel files, and the canal was irrigated with 1% sodium hypochlorite. A file, compatible with the channel gauge, was then used to measure the length of the root canal with the apex locator. Conventional radiographs were also performed and, using a millimeter endodontic ruler, the length of the canal was determined. The differences between the measurements obtained between the two methods were analyzed using the Student's t-test. Results: The mean canal length for conventional radiography was 9.83 mm and 9.67 mm for the apex locator. The results of this study did not show significant differences (p=0.641), independent of the presence or absence of physiological root resorption. Conclusion: The similarity in measurements obtained with X-ray or an apex locator indicates that it is not necessary to use X-rays as a complement to obtain the working length. The use of the apex locator can provide a quicker treatment, reducing the clinical time and stress of the child.
Subject(s)
Child, Preschool , Tooth, Deciduous , In Vitro Techniques/methods , Radiography, Dental/instrumentation , Tooth Apex/anatomy & histology , Odontometry/methods , Brazil , Data Interpretation, Statistical , EndodonticsABSTRACT
Abstract Objective: To evaluate the Antimicrobial Photodynamic Therapy (aPDT) in infected deciduous teeth by quantifying the viable bacteria in root canal treatment. Material and Methods: Radicular canal cultures were collected (n= 10). Four intra-canal samples were collected at four different times in each of the sampled teeth, as follows: Time 1 (T1), baseline: After opening the pulp-chamber; Time 2 (T2): After application of aPDT; Time 3 (T3): After mechanical, chemical manipulation; Time 4 (T4): After a second application of aPDT. The aPDT was performed with a 4J/cm energy low-intensity diode, together with 0.005% methylene blue as a photosensitizer. The clinical specimens were taken to the laboratory for a bacteria count (colony forming units) and the results were statistically analyzed using the Friedman and Wilcoxon tests, with a significance level of α=0.05. Results: Statistical differences were seen between the numbers of bacteria at times T1-T2, T1-T3 and T1-T4 on the cultivated plates. However, no significant statistical differences were observed between the number of bacteria in samples T2-T3, T2-T4 and T3-T4. Conclusion: Antimicrobial photodynamic therapy can be a good co-adjuvant in root canal decontamination of necrotic primary teeth.
Subject(s)
Humans , Male , Female , Child, Preschool , Photochemotherapy/instrumentation , Tooth, Deciduous , Dental Pulp Necrosis/diagnostic imaging , Low-Level Light Therapy/instrumentation , Brazil/epidemiology , Pilot Projects , Statistics, Nonparametric , Tooth, Nonvital/diagnosisABSTRACT
Abstract Objective: To discover the perceptions of pediatric patients, their parents and undergraduate Dentistry students of the use of the diode laser in frenectomy surgeries. Material and Methods: A qualitative study was conducted through semi-structured interviews, recorded and transcribed with subsequent content analysis. Twelve healthy children (5-8 years old) who needed a frenectomy were selected and invited, together with their parents, to undergo laser diode surgery. In addition, 28 undergraduate dentistry students were invited to attend the procedures. One week after performing the surgical procedures, the children (Group 1), their parents (Group 2) and the students who attended the procedures (Group 3) were individually interviewed for the thematic analysis. Results: The analysis of the conversations identified three emerging contents: positive thoughts on the use of diode laser; frustrations from the use of diode laser; and aspects related to professional training. Conclusion: The results pointed to the acceptance of surgical laser use in pediatric dentistry; however, the feelings of frustration indicate that its use requires guidance from the child and his/her parents, in addition to careful handling and specific training.
Subject(s)
Pediatric Dentistry , Laser Therapy/instrumentation , Lasers, Semiconductor/therapeutic use , Gingival Diseases/surgery , Labial Frenum/surgery , Perception , Brazil/epidemiology , Qualitative ResearchABSTRACT
Objetivo:O objetivo desta revisão foi realizar uma análise crítica da literatura quanto à utilização do procedimento enxerto de tecido conjuntivo e da membrana de fibrina rica em plaquetas para recobrimento da raiz exposta, frisando os benefícios e previsibilidade de sucesso de cada uma. Métodos:Foi realizada uma busca nas bases PubMed, SciELO e BIREME em março de 2019, sendo incluídos estudos relevantes relacionados ao tema para síntese deste trabalho. Resultados: Foram selecionados 5 artigos para serem incluídos nessa revisão crítica, sendo 3 ensaios clínicos controlados randomizados, 1 ensaio clínico e 1 um relato de caso. Conclusão:O recobrimento radicular com uso de enxerto de tecido conjuntivo e fibrina rica em plaquetas apresentou resultados satisfatórios, devolvendo estética, saúde e regeneração dos tecidos gengivais e periodontais. Em relação à fibrina rica em plaquetas, há poucos casos na literatura quanto aos resultados clínicos a longo prazo.
Aim: This review sought to perform a critical analysis of the literature regarding the use of the graft procedure of connective tissue and Platelet-rich fibrin to cover the exposed root, emphasizing the benefits and predictability of success of each. Methods: This study performed a search on the PubMed, SciELO, and BIREME databases, and included relevant studies related to the topic for the synthesis of this work. Results:Five articles were selected to be included in this critical review, three randomized controlled clinical trials, one clinical trial, and one case report. Conclusion:The root covering using a subepithelial connective tissue graft and platelet-rich fibrin presents satisfactory results, restoring esthetics and health, and regenerating the gingival and periodontal tissues. Regarding platelet-rich fibrin, there are few cases in the literature regarding long-term clinical outcomes.
Subject(s)
Connective Tissue , Platelet-Rich Fibrin , Gingival Recession , Transplantation, Autologous , Tissue TransplantationABSTRACT
BACKGROUND: Children with sickle cell disease may have their quality of life affected by oral alterations. However, there is still little data on oral health-related quality of life in these children. The aim of this study was to investigate the influence of sickle cell disease, socioeconomic characteristics, and oral conditions on oral health-related quality of life of children and teens. METHOD: One hundred and six children and teens with sickle cell disease were compared to a similar sample of 385 healthy peers. Data were collected through oral examinations, interviews to assess quality of life (Child Perceptions Questionnaire for children aged 8-10 and 11-14) and questionnaires containing questions on socioeconomic status. RESULTS: There were no statistically significant differences in the total scores of the Child Perceptions Questionnaires or domain scores comparing sickle cell disease patients to control subjects. When sub-scales were compared, oral symptoms and functional limitations had a greater negative impact on the quality of life of adolescents with sickle cell disease (p-value <0.001 and p-value <0.01, respectively) when compared to healthy controls. The only statistically significant determinants of negative impact on oral health-related quality of life in the overall sample was home overcrowding (more than two people/room) in the younger children's group, and dental malocclusion among teens. CONCLUSION: There was no significant difference in the negative impact on the oral health-related quality of life between the group with sickle cell disease and the control group. Of the oral alterations, there was a significant difference in the oral health-related quality of life between adolescents with sickle cell disease and controls only in relation to malocclusion. Among the socioeconomic characteristics, only overcrowding was significantly associated with a negative impact on oral health-related quality of life.
Subject(s)
Humans , Child , Adolescent , Anemia, Sickle Cell , Child , Malocclusion , Oral Health , Quality of LifeABSTRACT
BACKGROUND: Children with sickle cell disease may have their quality of life affected by oral alterations. However, there is still little data on oral health-related quality of life in these children. The aim of this study was to investigate the influence of sickle cell disease, socioeconomic characteristics, and oral conditions on oral health-related quality of life of children and teens. METHOD: One hundred and six children and teens with sickle cell disease were compared to a similar sample of 385 healthy peers. Data were collected through oral examinations, interviews to assess quality of life (Child Perceptions Questionnaire for children aged 8-10 and 11-14) and questionnaires containing questions on socioeconomic status. RESULTS: There were no statistically significant differences in the total scores of the Child Perceptions Questionnaires or domain scores comparing sickle cell disease patients to control subjects. When sub-scales were compared, oral symptoms and functional limitations had a greater negative impact on the quality of life of adolescents with sickle cell disease (p-value <0.001 and p-value <0.01, respectively) when compared to healthy controls. The only statistically significant determinants of negative impact on oral health-related quality of life in the overall sample was home overcrowding (more than two people/room) in the younger children's group, and dental malocclusion among teens. CONCLUSION: There was no significant difference in the negative impact on the oral health-related quality of life between the group with sickle cell disease and the control group. Of the oral alterations, there was a significant difference in the oral health-related quality of life between adolescents with sickle cell disease and controls only in relation to malocclusion. Among the socioeconomic characteristics, only overcrowding was significantly associated with a negative impact on oral health-related quality of life.
ABSTRACT
The aim of this study was to assess the impact of oral conditions of children with sickle cell disease (SCD) on their parents' quality of life (QoL). A cross-sectional study was performed with parents of outpatients suffering from SCD at a hematology referral center in Belo Horizonte, MG. A qualified dentist performed an intraoral exam. The Family Impact Scale (FIS) was used to assess the parents' perception of QoL. The parents answered some questions regarding sociodemographic and medical information about their children. The dmft/DMFT score, DAI, gum bleeding and SCD severity were evaluated in terms of their impacts on the overall mean FIS scores and subscale scores. The chance of more frequent impacts was greater in parents of adolescents (OR = 2.04; 95%CI = 1.2, 3.4) than of younger children. Dental caries (dmft/DMFT ≥ 1) had a negative impact on the QoL of parents of younger children and adolescents (p < 0.05 and p < 0.01, respectively). Among the parents of younger children, dental caries and SCD severity significantly affected the subscales for parental activities (PA) and parental emotions (PE) (p < 0.01, p < 0.05, respectively). Among parents of adolescents, dental caries (DMFT) and severe malocclusion adversely affected the PE and PA subscales (p < 0.01, p < 0.05, respectively). SCD severity affected the overall FIS score among young children's parents (p < 0.05). In conclusion, dental caries, age and SCD severity were associated with a negative impact on the QoL of parents of children with SCD.
Subject(s)
Anemia, Sickle Cell/psychology , Mouth Diseases/psychology , Oral Health/statistics & numerical data , Parents/psychology , Quality of Life/psychology , Sickness Impact Profile , Adolescent , Age Factors , Anemia, Sickle Cell/complications , Brazil , Caregivers/psychology , Child , Dental Caries/etiology , Dental Caries/psychology , Epidemiologic Methods , Female , Humans , Male , Malocclusion/etiology , Malocclusion/psychology , Mouth Diseases/etiology , PsychometricsABSTRACT
Abstract The aim of this study was to assess the impact of oral conditions of children with sickle cell disease (SCD) on their parents' quality of life (QoL). A cross-sectional study was performed with parents of outpatients suffering from SCD at a hematology referral center in Belo Horizonte, MG. A qualified dentist performed an intraoral exam. The Family Impact Scale (FIS) was used to assess the parents' perception of QoL. The parents answered some questions regarding sociodemographic and medical information about their children. The dmft/DMFT score, DAI, gum bleeding and SCD severity were evaluated in terms of their impacts on the overall mean FIS scores and subscale scores. The chance of more frequent impacts was greater in parents of adolescents (OR = 2.04; 95%CI = 1.2, 3.4) than of younger children. Dental caries (dmft/DMFT ≥ 1) had a negative impact on the QoL of parents of younger children and adolescents (p < 0.05 and p < 0.01, respectively). Among the parents of younger children, dental caries and SCD severity significantly affected the subscales for parental activities (PA) and parental emotions (PE) (p < 0.01, p < 0.05, respectively). Among parents of adolescents, dental caries (DMFT) and severe malocclusion adversely affected the PE and PA subscales (p < 0.01, p < 0.05, respectively). SCD severity affected the overall FIS score among young children's parents (p < 0.05). In conclusion, dental caries, age and SCD severity were associated with a negative impact on the QoL of parents of children with SCD
Subject(s)
Humans , Male , Female , Child , Adolescent , Parents/psychology , Quality of Life/psychology , Oral Health/statistics & numerical data , Sickness Impact Profile , Anemia, Sickle Cell/psychology , Mouth Diseases/psychology , Psychometrics , Brazil , Epidemiologic Methods , Age Factors , Caregivers/psychology , Dental Caries/etiology , Dental Caries/psychology , Anemia, Sickle Cell/complications , Malocclusion/etiology , Malocclusion/psychology , Mouth Diseases/etiologyABSTRACT
Although male crickets provide more informative characters for the delimitation of species and genera, many taxonomic descriptions are based only on females. This is the case for Argizala Walker, 1869 and its two valid species, A. brasiliensis Walker, 1869 and A. hebardi Rehn, 1915. We provide herein a redescription of A. brasiliensis based on a male collected in the Pampa Biome, Capão do Leão municipality, Rio Grande do Sul State, Brazil. We present photographs of epitype and male genitalia, and discuss the diagnostic features of the genus and its morphological proximity to other Pteronemobiini Nearctic genera.
Subject(s)
Gryllidae/classification , Animal Structures/anatomy & histology , Animal Structures/growth & development , Animals , Body Size , Brazil , Female , Gryllidae/anatomy & histology , Gryllidae/growth & development , Male , Organ SizeABSTRACT
BACKGROUND: Children with sickle cell disease (SCD) may present oral conditions that can compromise children's health even more. However, there is still no consensus on the association between SCD and dental caries. The aim of this study was to assess caries prevalence in children with sickle cell disease (SCD), and the association of dental caries with socioeconomic factors, disease severity, and oral-health related to quality of life (OHRQoL). METHODS: The sample was comprised of 106 children with SCD aged 8 to 14 years who were attending the Center for Hematology (Hemominas) in Belo Horizonte, Brazil. They were matched to 385 healthy peers. Data collection included interviews with guardians concerning SCD characteristics, and previous social and oral examinations to determine the caries prevalence. Caries prevalence as measured through the Decayed, Missing and Filled (dmft and DMFT) indices. OHRQoL was evaluated through the Brazilian versions of the Child Perceptions Questionnaires (CPQ8-10, and CPQ11-14 short-form version). Statistical analyses were performed using the chi-square test or Fisher`s exact test and the Mann Whitney test, as well as linear regression. RESULTS: The DMFT index was 1.3 (SD: 2.1) in younger children with SCD and 1.5 (SD: 1.9) in SCD teens. Younger children with SCD had lower caries experience compared to healthy peers (p = .03). The experience of dental caries among teens with SCD was similar to healthy peers (p > 0.05). In addition, we did not see a significant difference on the mean overall scores of CPQ8-10 between SCD younger children and controls. There was no statistically significant difference in the mean overall scores of teens CPQ11-14 between SCD and the control group. Socioeconomic variables were not associated with dental caries in the participants with SCD. However, SCD severity was associated with higher DMFT indexes (p < 0.05). CONCLUSIONS: Younger children with SCD had a low experience of dental caries. The dental caries experience in teenagers with SCD was similar to their healthy peers. OHRQoL was similar between SCD participants and controls.
Subject(s)
Anemia, Sickle Cell/epidemiology , Dental Caries/epidemiology , Oral Health , Quality of Life , Adolescent , Adolescent Health/statistics & numerical data , Anemia, Sickle Cell/psychology , Brazil/epidemiology , Child , Child Health/statistics & numerical data , Cross-Sectional Studies , DMF Index , Dental Caries/psychology , Female , Humans , Income/statistics & numerical data , Male , Oral Health/statistics & numerical data , Periodontal Index , Prevalence , Socioeconomic FactorsABSTRACT
O objetivo deste estudo foi determinar a correlação filho-pais na avaliação da qualidade de vida relacionada à saúde bucal de crianças e de suas famílias e determinar as características associadas a essa correlação em uma população pediátrica com anemia falciforme. Foi realizado um estudo transversal com 106 crianças, de 8 a 14 anos, com diagnóstico de anemia falciforme (AF), no estado de Minas Gerais, Brasil, e seus pais/cuidadores. Estas crianças estavam fazendo acompanhamento de saúde na Fundação Centro de Hematologia e Hemoterapia de Minas Gerais (Hemominas). A qualidade de vida relacionada à saúde bucal (QVRSB) foi mensurada, utilizando-se as versões brasileiras dos instrumentos Child Perception Questionnaire para crianças de 8 a 10 anos (CPQ8-10) e de 11 a 14 anos (versão curta do CPQ11-14), o Parental-Caregiver Perceptions Questionnaire (P-CPQ) e o Family Impact Scale (FIS). Um dentista calibrado realizou os exames clínicos bucais para avaliação da experiência de cárie dentária, má oclus¿o e sangramento gengival, segundo os critérios da Organização Mundial de Saúde (OMS). As informações sobre a AF, as características sócio-demográficas e econômicas foram obtidas por meio de entrevistas realizadas com os pais/responsáveis pelas crianças. Os dados foram analisados por meio do teste de correlação de Spearman e regressão linear. Houve uma forte correlação entre P-CPQ e FIS (rs=0,732). Foi observada correlação moderada entre as avaliações de QVRSB realizadas pela criança (CPQ) e pelo pai-proxy (P-CPQ) (rs=0,433). Ao se analisar a correlação entre a avaliação da QVRSB realizada pelas crianças (CPQ) e o impacto na família (FIS) relatado pelos pais, observou-se uma correlação fraca (rs=0,331). Maiores diferenças no z-escore entre P-CPQ e FIS foram associadas com a menor idade do filho (p<0,01), mais baixa espiritualidade/religiosidade (p<0,05), menor renda familiar (p<0,01) e severidade da má oclusão dentária (p<0,05)...
More recently, there has been increasing interest in the oral health-related quality of life (OHRQoL) of children, since pediatric oral disorders are likely to have a negative effect on the child quality of life (QoL). One issue that receives a great deal of attention is the comparison of the measurement of children OHRQoL reports with those of their parents. In this way, three systematic reviews were carried out to review the literature on valid and reliable informations from children and parents concerning child OHRQoL, and to identify the pattern of agreement/disagreement between their reports. The literature was searched using Medline, ISI, Lilacs and Scielo, from 1985-2007. Two researchers independently checked and then selected only articles that used well-validated instruments, provided quantitative measurements of child clinical oral health status, and presented children and parental perceptions of child OHRQoL. In the first systematic review, from 89 records found, thirteen fulfilled the criteria. All selected studies suggested good construct validity. However, child understanding of oral health and well-being are affected by age, age-related experiences, gender, race, education, culture, experiences related to oral conditions, opportunities for treatment, childhood period of changes, back-translating questionnaire and child self-perceived treatment need. Twelve of 402 articles originally identified were included in the second systematic review. The results showed that the relationships between clinical oral health status and QoL in children were not direct, but mediated by a variety of personal, social and environmental variables, as well as by the child development, which have influence on the comprehension about the relationship among health, illness and QoL. In the third one, out of 87 articles that were critically assessed, five studies were selected ...
Subject(s)
Humans , Male , Female , Child , Adolescent , Adult , Anemia, Sickle Cell , Oral Health , Father-Child Relations , Quality of LifeABSTRACT
Extracellular lipases from the endophytic yeast Candida guilliermondii isolated from castor leaves (Ricinus communis L.) were produced using low-cost raw materials such as agro-industrial residues and applying them in the esterification of oleic acid for evaluating their potential use in biodiesel production. After partial purification using ammonium sulfate, the enzyme was characterized and presented higher activity (26.8 ± 1.5 U mL-1) in the presence of 5 mmol L-1 NaCl at 30 ºC and pH 6.5. The production through submerged fermentation was formerly performed in 150 mL erlenmeyer flasks and, once the enzyme production was verified, assays in a 14 L bioreactor were conducted, obtaining 18 ± 1.4 U mL-1. The produced enzyme was applied in the oleic acid esterification under different solvents: hexane, cyclohexane or cyclohexanone) and different acid:alcohol molar ratios. Higher ester conversion rate (81%) was obtained using hexane and the molar ratio of 1:9 was the best conditions using methanol. The results suggest the potential for development of endophytic yeast in the production of biocatalyst through submerged fermentation using agroindustrial residues as culture medium.
Subject(s)
Candida/enzymology , Candida/metabolism , Lipase/isolation & purification , Lipase/metabolism , Candida/growth & development , Candida/isolation & purification , Esterification , Endophytes/enzymology , Hydrogen-Ion Concentration , Oleic Acid/metabolism , Plant Leaves/microbiology , Ricinus/microbiology , Sodium Chloride/metabolismABSTRACT
Extracellular lipases from the endophytic yeast Candida guilliermondii isolated from castor leaves (Ricinus communis L.) were produced using low-cost raw materials such as agro-industrial residues and applying them in the esterification of oleic acid for evaluating their potential use in biodiesel production. After partial purification using ammonium sulfate, the enzyme was characterized and presented higher activity (26.8 ± 1.5 U mL-1) in the presence of 5 mmol L-1 NaCl at 30 ºC and pH 6.5. The production through submerged fermentation was formerly performed in 150 mL erlenmeyer flasks and, once the enzyme production was verified, assays in a 14 L bioreactor were conducted, obtaining 18 ± 1.4 U mL-1. The produced enzyme was applied in the oleic acid esterification under different solvents: hexane, cyclohexane or cyclohexanone) and different acid:alcohol molar ratios. Higher ester conversion rate (81%) was obtained using hexane and the molar ratio of 1:9 was the best conditions using methanol. The results suggest the potential for development of endophytic yeast in the production of biocatalyst through submerged fermentation using agroindustrial residues as culture medium.
Subject(s)
Candida/enzymology , Candida/metabolism , Lipase/isolation & purification , Lipase/metabolism , Candida/growth & development , Candida/isolation & purification , Culture Media/chemistry , Esterification , Endophytes/enzymology , Endophytes/growth & development , Endophytes/isolation & purification , Endophytes/metabolism , Hydrogen-Ion Concentration , Oleic Acid/metabolism , Plant Leaves/microbiology , Ricinus/microbiology , Sodium Chloride/metabolism , TemperatureABSTRACT
Extracellular lipases from the endophytic yeast Candida guilliermondii isolated from castor leaves (Ricinus communis L.) were produced using low-cost raw materials such as agro-industrial residues and applying them in the esterification of oleic acid for evaluating their potential use in biodiesel production. After partial purification using ammonium sulfate, the enzyme was characterized and presented higher activity (26.8 ± 1.5 U mL(-1)) in the presence of 5 mmol L(-1) NaCl at 30 °C and pH 6.5. The production through submerged fermentation was formerly performed in 150 mL erlenmeyer flasks and, once the enzyme production was verified, assays in a 14 L bioreactor were conducted, obtaining 18 ± 1.4 U mL(-1). The produced enzyme was applied in the oleic acid esterification under different solvents: hexane, cyclohexane or cyclohexanone) and different acid:alcohol molar ratios. Higher ester conversion rate (81%) was obtained using hexane and the molar ratio of 1:9 was the best conditions using methanol. The results suggest the potential for development of endophytic yeast in the production of biocatalyst through submerged fermentation using agroindustrial residues as culture medium.
Subject(s)
Candida/enzymology , Candida/metabolism , Lipase/isolation & purification , Lipase/metabolism , Candida/growth & development , Candida/isolation & purification , Culture Media/chemistry , Endophytes/enzymology , Endophytes/growth & development , Endophytes/isolation & purification , Endophytes/metabolism , Esterification , Hydrogen-Ion Concentration , Oleic Acid/metabolism , Plant Leaves/microbiology , Ricinus/microbiology , Sodium Chloride/metabolism , TemperatureABSTRACT
Objetivo: Avaliar o impacto da cárie e da maloclusão (necessidade etratamento ortodôntico) na qualidade de vida de escolares de oito a 14 anos de idade residentes em Belo Horizonte, Minas Gerais. Métodos: Realizou-se um estudo transversal com amostra deconveniência (n=75), dividida em dois grupos de acordo com a idade dos escolares: grupo 1 oito a dez anos e grupo 2- 11 a 14 anos. Os dados foram coletados pelo exame clínico para diagnóstico da cárie dentária (CPOD) e avaliação da necessidade de tratamento ortodôntico (IED) e aplicação dos instrumentos CPQ8-10 e CPQ11-14, para avaliação do impacto na qualidade de vida. Realizou-se análise descritiva, teste qui-quadrado e Mann-Whitney.Resultados: No grupo de estudantes com idade entre oito e dez anos, observou-se associação estatisticamente significativa entre necessidade de tratamento ortodôntico (IED > 30) e impacto negativo na qualidade de vida (p = 0,004), principalmente nos domínios limitação funcional (p<0,001), bem-estar emocional (p=0,001) e bem-estar social (p=0,008). Não foi observada associação entre a cárie dentária e impacto negativo na qualidade de vida neste grupo (p=0,66). Entre as crianças com idade entre 11 e 14 anos, verificou-se associação estatisticamente significativa entre impacto na qualidade de vida, cárie (p=0,004) e necessidade de tratamento ortodôntico (p=0,001). Considerando-se a cárie, a associação foi observada com os domínios bem-estar emocional (p=0,006) e social (p=0,02). A necessidade de tratamento ortodôntico mostrou associação significativa com limitação funcional (p=0,02), bemestar emocional (p=0,05) e social (p=0,03). Conclusão: A maloclusão (necessidade de tratamento ortodôntico) mostrou associação com impacto negativo na qualidade e vida de estudantes na faixa etária entre oito e 14 anos. Impacto negativo também foi observado entre estudantes de 11 a 14 anos queapresentavam cárie dentária
Objective: To evaluate the impact of dental caries and malocclusion(need of orthodontic treatment) on the quality of life of schoolchildrenaged 8 to 14 years living in the city of Belo Horizonte, MG, Brazil.Method: This study was a cross-sectional investigation with aconvenience sample (n=75) divided into two groups, according to theage of schoolchildren: Group 1: 8 to 10 years and Group 2: 11 to 14years. Data were collected by clinical examination for diagnosis ofdental caries (DMFT index) and determination of orthodontic treatmentneed (DAI - dental aesthetic index), and through application of CPQ8-10and CPQ11-14 instruments to assess the influence on the quality of life.Descriptive analysis, Chi-square and Mann-Whitney tests wereperformed.Results: In the group of schoolchildren aged 8 to 10 years, there was astatistically significant association between the need of orthodontictreatment (DAI>30) and negative impact on the quality of life (p=0.004),especially for the domains functional limitation (p<0.001), emotionalwell being (p=0.001) and social well being (p=0.008).
No associationwas found between dental caries and negative impact on quality of lifein this group (p=0.66). In the group of schoolchildren aged 11 to 14years there was a statistically significant association between impact on the quality of life and dental caries (p=0.004) and need of orthodontictreatment (p<0.001). Regarding dental caries, there was significantassociation between the domains emotional well being (p=0.006) andsocial well being (p=0.02). The need of orthodontic treatmentshowed a significant association with functional limitation (p=0.02),emotional well being (p=0.05) and social well being (p=0.03).Conclusion: Malocclusion (need of orthodontic treatment) wasassociated with negative impact on the quality of life of schoolchildrenin the age range between 8 and 14 years. There was also negativeimpact on the quality of life of 11-14 children with dental caries
Subject(s)
Child , Adolescent , Dental Caries/diagnosis , Students/psychology , Malocclusion/diagnosis , Quality of Life , Statistics, Nonparametric , Cross-Sectional Studies , Surveys and QuestionnairesABSTRACT
Three new Neotropical species of Hygronemobius Hebard are described from Atlantic Forest remnants in southeastern Brazil: Hygronemnobius indaia sp. n. and Hygroneinobius iperoigae sp. n. from the state of São Paulo and Hygroneinobius guriri sp. n. from the state of Espírito Santo. We provide photographs of types and male genitalia, and discuss the morphology of male genitalia, taxonomy, calling songs and biogeographic distribution of the genus.
Subject(s)
Orthoptera/classification , Animal Distribution , Animal Structures/anatomy & histology , Animal Structures/growth & development , Animals , Body Size , Brazil , Female , Genitalia, Male/anatomy & histology , Genitalia, Male/growth & development , Male , Organ Size , Orthoptera/anatomy & histology , Orthoptera/growth & developmentABSTRACT
Objetivo: Verificar la interferencia del embarazo en las percepciones en salud bucal de adolescentes embarazadas de 12 a 18 años, con baja condición socio-económica, residentes en Belo Horizonte, Minas Gerais. Material y Métodos: Se realizó un estudio cualitativo con las adolescentes (n=60) divididas en dos grupos: embarazada (n=30) o no embarazada (n=30). Entre las gestantes, diez estaban en el primer trimestre de gestación, trece en el segundo, y siete en el último trimestre de gestación. Los datos fueron recolectados a través de entrevistas individuales. Se realizó el análisis del contenido. Resultados: Tres categorías de análisis surgieron a través del contenido manifestado en ambos grupos: consciencia de determinantes de la salud bucal (conocimientos y actitudes), reacciones emocionales relativas a la salud bucal (sentimientos positivos, pasividad y sentimientos negativos) y valores en salud bucal (apariencia, función social de los padres). Conclusión: Entre las jóvenes se observó que la experiencia del embarazo no interfirió en las percepciones en salud bucal.
Objetivo: verificar a interferencia da gravidez nas percepções em saúde bucal de adolescentes grávidas, com idades entre 12 e 18 anos, com baixa inserção sócio-econômica, residentes em Belo Horizonte, Minas Gerais. Método: Realizou-se um estudo qualitativo com adolescentes (n=60) divididas em dois grupos de acordo com a experiência de gravidez (n=30) ou não (n=30). Entre as gestantes, dez estavam no primeiro trimestre de gravidez, 13 no segundo trimestre e sete no último trimestre de gestação. Os dados foram coletados através de entrevistas individuais. Realizou-se a análise de conteúdo. Resultados: Três categorais de análise emergiram através do conteúdo manifesto em ambos os grupos: consciências de determinantes da saúde bucal (conhecimentos e atitudes), reações emocionais relativas à saúde bucal (sentimentos positivos, passividade e sentimentos negativos) e valores em saúde bucal (aparência, função social dos pais). Conclusão: Entre as jovens observouse que a experiência da gravidez não interferiu nas percepções em saúde bucal.
Objective: Check the interference of pregnancy on perceptions of oral health in poor pregnant aged 12-18 years old living in Belo Horizonte, Minas Gerais. Methods: A qualitative study was carried with teenagers (n=60) divided into two groups according to the pregnancy experience (n=30) or not (n=30). Among pregnant woman, 10 were in the first trimester, 13 in the second and seven in the last trimester of pregnancy. Data were collected through individual interviews. Content analysis was performed. Results: Three categories of the manifest content analysis appeared in both groups: awareness of oral health determinants (knowledge and attitudes), emotional reactions related to oral health (positive feelings, negative feelings and passivity) and values in oral health (appearance, social function of parents). Conclusion: Among the children analyzed, pregnancy experience did not influence the perceptions of oral health.
Subject(s)
Humans , Pregnancy , Adolescent , Oral Health , Social Class , Comparative Study , Insurance BenefitsABSTRACT
A displasia ectodérmica (DE) compreende um grupo grande e heterogêneo de doenças hereditáriasque se caracteriza por apresentar manifestações clínicas relacionadas às anomalias das estruturas de origemectodérmica, principalmente nos cabelos, unhas, dentes e pele. Este trabalho descreve o caso clínico de umpaciente do sexo masculino de 11 anos de idade, que compareceu à clínica odontológica de uma instituição deensino superior de Belo Horizonte MG. A criança apresentava-se com características da displasia ectodérmica,na forma hipoidrótica, e com história de diversos indivíduos afetados na família materna. Fez-se, ainda, revisãoda literatura e discussão da etiologia e tratamento para o caso descrito.