ABSTRACT
Anonymous DNA probes mapping to human chromosome 16 and the distal region of the human X chromosome were isolated from a genomic library constructed using lambda EMBL3 and DNA from a mouse/human hybrid. The hybrid cell contained a der(16)t(X;16)(q26;q24) as the only human chromosome. Fifty clones were isolated using total human DNA as a hybridisation probe. Forty six clones contained single copy DNA in addition to the repetitive DNA. Pre-reassociation with sonicated human DNA was used to map these clones by a combination of Southern blot analysis of a hybrid cell panel containing fragments of chromosomes 16 and X and in situ hybridisation. One clone mapped to 16pter----16p13.11, one clone to 16p13.3----16p13.11, four clones to 16p13.3----16p13.13, two clones to 16p13.13----16p13.11, one clone to 16p13.11, seven clones to 16p13.11----16q12 or 16q13, four clones to 16q12 or 16q13, three clones to 16q13----16q22.1, four clones to 16q22.105----16q24, and nineteen clones to Xq26----Xqter. Two clones mapping to 16p13 detected RFLPs. VK5 (D16S94) detected an MspI RFLP, PIC 0.37. VK20 (D16S96) detected a TaqI RFLP, PIC 0.37 and two MspI RFLPs, PIC 0.30 and 0.50. The adult polycystic kidney disease locus (PKD1) has also been assigned to 16p13. The RFLPs described will be of use for genetic counselling and in the isolation of the PKD1 gene. Similarly, the X clones may be used to isolate RFLPs for genetic counselling and the isolation of genes for the many diseases that map to Xq26----qter.
Subject(s)
Chromosomes, Human, Pair 16/ultrastructure , DNA Probes , Genetic Markers , X Chromosome/ultrastructure , Animals , Chromosome Mapping , Fragile X Syndrome/genetics , Humans , Hybrid Cells , Mice , Polymorphism, Restriction Fragment LengthABSTRACT
The gene for human interleukin 7 (IL7) maps to chromosome 8 by Southern analysis of a somatic cell hybrid panel and to 8q12-q13 by in situ hybridization.
Subject(s)
Chromosomes, Human, Pair 8 , Interleukins/genetics , Blotting, Southern , Chromosome Banding , Chromosome Mapping , Humans , Interleukin-7ABSTRACT
Physical mapping of 13 different breakpoints on the short arm of chromosome 16 using previously mapped probes and the subsequent mapping of additional probes enabled the division of this portion of the chromosome into six different intervals. D16S94 was mapped between HBA and D16S80 and is closer to PKD1 than either HBA or D16S80. A tight linkage group which includes FRA16A, D16S8, and D16S79 was identified. Seven breakpoints, including FRA16A, could not be separated by probe localizations. This study provides the basis for the development of detailed maps of the short arm of chromosome 16.
Subject(s)
Chromosomes, Human, Pair 16 , Animals , Blotting, Southern , Chromosome Mapping , Chromosomes, Human, Pair 16/ultrastructure , DNA Probes , Genetic Linkage , Humans , Hybrid Cells , Mice , Nucleic Acid HybridizationABSTRACT
The gene encoding the human secreted carbonic anhydrase isozyme CAVI(CA6) maps to chromosome 1 by Southern analysis of a somatic cell hybrid panel and to 1p36.22----p36.33 by in situ hybridization. CA6 is therefore not linked to the cytoplasmic carbonic anhydrase genes on chromosome 8 or to CA7 on chromosome 16.
