ABSTRACT
No disponible
Subject(s)
Humans , Male , Female , Infant , Child, Preschool , Enterovirus A, Human/isolation & purification , Enterovirus Infections/epidemiology , Respiratory Tract Infections/microbiology , Nucleic Acid Amplification Techniques , Enterovirus A, Human/pathogenicityABSTRACT
No disponible
Subject(s)
Humans , Male , Female , Child, Preschool , Child , Respiratory Tract Infections/diagnosis , Respiratory Tract Infections/drug therapy , Respiratory Tract Infections/microbiology , Enterovirus Infections/classification , Enterovirus Infections/drug therapy , Enterovirus Infections/microbiology , Enterovirus/classification , Enterovirus/isolation & purification , Enterovirus Infections/pathology , Enterovirus/pathogenicity , EnterovirusSubject(s)
Enterovirus A, Human , Enterovirus Infections/diagnosis , Child, Preschool , Female , Humans , Infant , Male , SpainSubject(s)
Enterovirus D, Human , Enterovirus Infections/complications , Respiratory Tract Infections/virology , Acute Disease , Child , Child, Preschool , Community-Acquired Infections/virology , Enterovirus Infections/diagnosis , Female , Humans , Infant , Male , Respiratory Tract Infections/diagnosisABSTRACT
No disponible
Subject(s)
Humans , Infant , Respiratory Syncytial Viruses/pathogenicity , Respiratory Syncytial Virus Infections/complications , Conjunctivitis, Viral/microbiology , Polymerase Chain Reaction/methodsSubject(s)
Conjunctivitis, Viral/virology , Respiratory Syncytial Virus Infections/virology , Respiratory Syncytial Virus, Human/isolation & purification , Acute Disease , Conjunctivitis, Viral/epidemiology , Conjunctivitis, Viral/transmission , Female , Humans , Infant , Lacrimal Apparatus/virology , Male , Respiratory Syncytial Virus Infections/epidemiology , Respiratory Syncytial Virus Infections/transmission , Respiratory Syncytial Virus, Human/classification , SeasonsSubject(s)
Croup/virology , Cytomegalovirus Infections/diagnosis , Tracheitis/virology , Acute Disease , Combined Modality Therapy , Croup/drug therapy , Croup/therapy , Cytomegalovirus/immunology , Cytomegalovirus Infections/complications , Cytomegalovirus Infections/drug therapy , Dexamethasone/therapeutic use , Diagnosis, Differential , Emergencies , Fluid Therapy , Humans , Immunoglobulin M/blood , Infant , Male , Oxygen Inhalation Therapy , Respiratory Sounds/etiology , Respirovirus Infections/diagnosis , Tracheitis/drug therapy , Tracheitis/therapySubject(s)
Humans , Male , Female , Infant , Influenza, Human/virology , Influenza A Virus, H1N1 Subtype/isolation & purification , Influenza A Virus, H3N2 Subtype/isolation & purification , Influenza B virus/isolation & purification , Peru/ethnology , Polymerase Chain Reaction , Postoperative Complications/virology , Seasons , Spain , Neuroectodermal Tumors, Primitive/complications , Neuroectodermal Tumors, Primitive/surgery , Influenza, Human/complications , Influenza, Human/diagnosis , Influenza, Human/epidemiology , Disease Outbreaks , Emergencies , Brain Neoplasms/complications , Brain Neoplasms/surgerySubject(s)
Influenza A Virus, H1N1 Subtype/isolation & purification , Influenza A Virus, H3N2 Subtype/isolation & purification , Influenza B virus/isolation & purification , Influenza, Human/virology , Brain Neoplasms/complications , Brain Neoplasms/surgery , Disease Outbreaks , Emergencies , Female , Humans , Infant , Influenza, Human/complications , Influenza, Human/diagnosis , Influenza, Human/epidemiology , Male , Neuroectodermal Tumors, Primitive/complications , Neuroectodermal Tumors, Primitive/surgery , Peru/ethnology , Polymerase Chain Reaction , Postoperative Complications/virology , Seasons , SpainABSTRACT
INTRODUCTION: Human Metapneumovirus (hMPV) was first identified in 2001 in respiratory samples from children and adults with acute respiratory tract infection. The aim of this prospective study was to determine the clinical and epidemiological characteristics of pediatric patients with an acute respiratory tract infection and exclusive isolation of hMPV in respiratory samples (December 2005-January 2007). MATERIAL AND METHODS: All respiratory tract samples were submitted to rapid antigen detection against respiratory syncytial virus (RSV) and influenza A and B viruses. To isolate respiratory viruses, samples were inoculated in various cell lines using the shell vial culture assay. To isolate hMPV, the LLC-MK2 cell line was used. Only antigen-negative samples were studied for the presence of hMPV. RESULTS: Over the study period, 32 hMPV were isolated from different patients, accounting for 1.7% of all samples studied for this virus (only RSV-negative samples, 1,791) and 1.5% of all samples studied. Peak incidence was found between December and March of the two years studied. Of the 32 patients in whom hMPV was detected, 17 (53.2%) were female and 15 (46.8%) male. Mean age was 12.5 months (range, 1 month-4 years). The most frequent clinical symptoms were fever (90.6%), cough (87.5%), rhinorrhea and bronchiolitis (46.8%). Three patients (9.4%) were hospitalized. CONCLUSIONS: Respiratory infection caused by hMPV is considered an emergent disease in pediatric patients. The clinical manifestations are very similar to those of RSV infection; hence, only virological study can establish the definite etiological diagnosis.
Subject(s)
Communicable Diseases, Emerging/epidemiology , Metapneumovirus/pathogenicity , Paramyxoviridae Infections/epidemiology , Respiratory Tract Infections/epidemiology , Acute Disease , Antigens, Viral/analysis , Asthma/epidemiology , Child, Preschool , Communicable Diseases, Emerging/virology , Comorbidity , Cough/etiology , Diagnosis, Differential , Female , Fever/etiology , Humans , Incidence , Infant , Infant, Newborn , Male , Metapneumovirus/immunology , Metapneumovirus/isolation & purification , Paramyxoviridae Infections/diagnosis , Paramyxoviridae Infections/virology , Prospective Studies , Respiratory Syncytial Virus Infections/diagnosis , Respiratory Tract Infections/virologyABSTRACT
Introducción. El metapneumovirus humano (MPVh) fue identificado por primera vez en 2001 a partir de aspirados nasofaríngeos de niños y adultos con infección aguda del tracto respiratorio. El objetivo de este estudio prospectivo es conocer las características clínicas y epidemiológicas de los pacientes pediátricos con infección respiratoria aguda y aislamiento exclusivo del MPVh (diciembre 2005-enero 2007). Material y métodos. Las muestras fueron sometidas a la detección antigénica rápida frente al virus respiratorio sincitial (VRS) y los virus gripales A y B. Las muestras fueron sembradas en shell-viales de diferentes líneas celulares para el aislamiento de virus respiratorios. Para el MPVh se utilizó la línea LLC-MK2. Sólo las muestras negativas en las técnicas antigénicas fueron estudiadas para la presencia del MPVh. Resultados. En este estudio se han aislado 32 MPVh de pacientes distintos, que representan el 1,7% de todas las muestras estudiadas para este virus (sólo las negativas al VRS: 1.791) y el 1,5% de todas las muestras analizadas. La mayoría de casos se presentaron entre los meses de diciembre y marzo de ambos años estudiados. Los 32 casos correspondían a 17 niñas (53,2%) y 15 niños (46,8%). La edad media de los pacientes fue de 12,5 meses (intervalo: 1 mes y 4 años). La mayoría de los pacientes presentaron fiebre (90,6%), tos (87,5%), rinorrea y bronquiolitis (46,8%). Sólo 3 pacientes (9,4%) precisaron de ingreso hospitalario. Conclusiones. Las infecciones respiratorias causadas por el MPVh se han convertido en una enfermedad emergente en la población pediátrica. Las manifestaciones clínicas no permiten realizar un diagnóstico diferencial con el VRS, de modo que sólo el estudio virológico permitirá establecer el diagnóstico etiológico definitivo (AU)
Introduction. Human Metapneumovirus (hMPV) was first identified in 2001 in respiratory samples from children and adults with acute respiratory tract infection. The aim of this prospective study was to determine the clinical and epidemiological characteristics of pediatric patients with an acute respiratory tract infection and exclusive isolation of hMPV in respiratory samples (December 2005-January 2007). Material and methods. All respiratory tract samples were submitted to rapid antigen detection against respiratory syncytial virus (RSV) and influenza A and B viruses. To isolate respiratory viruses, samples were inoculated in various cell lines using the shell vial culture assay. To isolate hMPV, the LLC-MK2 cell line was used. Only antigen-negative samples were studied for the presence of hMPV. Results. Over the study period, 32 hMPV were isolated from different patients, accounting for 1.7% of all samples studied for this virus (only RSV-negative samples, 1,791) and 1.5% of all samples studied. Peak incidence was found between December and March of the two years studied. Of the 32 patients in whom hMPV was detected, 17 (53.2%) were female and 15 (46.8%) male. Mean age was 12.5 months (range, 1 month-4 years). The most frequent clinical symptoms were fever (90.6%), cough (87.5%), rhinorrhea and bronchiolitis (46.8%). Three patients (9.4%) were hospitalized. Conclusions. Respiratory infection caused by hMPV is considered an emergent disease in pediatric patients. The clinical manifestations are very similar to those of RSV infection; hence, only virological study can establish the definite etiological diagnosis (AU)
Subject(s)
Humans , Male , Female , Infant , Child, Preschool , Child , Adolescent , Metapneumovirus/isolation & purification , Paramyxoviridae Infections/epidemiology , Respiratory Tract Infections/epidemiology , Metapneumovirus/pathogenicity , Prospective Studies , Paramyxoviridae Infections/virology , Respiratory Tract Infections/virology , Communicable Diseases, Emerging/epidemiology , Diagnosis, DifferentialABSTRACT
We report a prospective study concerning the efficacy of LLC-MK2 (continuous monkey kidney cell), Hep-2, MDCK (Madin-Darby Canine Kidney), Vero and MRC-5 cell lines, by shell vial assay, and incubation time in the isolation of hMPV from pediatric respiratory samples. The overall sensitivity of the cell lines studied were: 100% for the LLC-MK2, 68.7% for the Hep-2, 28.1% for the Vero, 3.1% for the MDCK and 0% for the MRC-5. Only one strain (3.1%) showed growth in the four cell lines studied and 10 (31.2%) strains only grew in the LLC-MK2 cell line. The analysis of incubation times showed that only 14 strains (43.7%) were able to grow after 3 days of incubation, while all strains (100%) showed growth after 5 days. The use of shell vials with commercial LLC-MK2 cells could be a method for isolating hMPV from respiratory samples in the pediatric population.
Subject(s)
Metapneumovirus/isolation & purification , Nasopharynx/virology , Paramyxoviridae Infections/virology , Respiratory Tract Infections/virology , Virus Cultivation , Animals , Cell Line , Child , Humans , Metapneumovirus/growth & development , Paramyxoviridae Infections/diagnosis , Prospective Studies , Respiratory Tract Infections/diagnosis , Time FactorsABSTRACT
OBJETIVOS. Estudiar prospectivamente la emergencia y la problemática diagnóstica de las bronquiolitis causadas por el virus influenza A en la población infantil durante la epidemia gripal de 1999-2000. MATERIAL Y MÉTODOS. Las muestras del virus respiratorio sincitial (VRS)-negativas (ELISA dot-blot) fueron sembradas en la línea celular MDCK para el aislamiento del virus influenza A, mediante la técnica shell-vial. Los viales fueron revelados mediante una inmunofluorescencia indirecta con anticuerpos dirigidos contra la nucleoproteína del virus influenza A (Monofluokit IA, Pasteur Diagnostics). Se estudiaron las características clínicas y epidemiológicas de los pacientes con aislamiento vírico. Se ha realizado un estudio teórico coste-beneficio sobre la utilidad de la detección antigénica rápida del virus influenza A. RESULTADOS. Se han detectado 177 casos de infección respiratoria causada por el virus influenza A. En 62 casos (52,9 por ciento) el diagnóstico fue de bronquiolitis y de ellos el 80,6 por ciento de los casos eran menores de 6 meses. El subtipo H3N2 ha predominado en todas las infecciones respiratorias y particularmente en las bronquiolitis. En la temporada 1999-2000 se detectó una emergencia significativa de las bronquiolitis causadas por el virus influenza A (semana 3, 30 por ciento casos influenza A frente 24 por ciento casos VRS). El estudio de coste muestra que la detección antigénica de los VRS e influenza A en todas las muestras respiratorias determinaría un coste de unas 5.130 pts por muestra. CONCLUSIONES. Se ha observado y confirmado en la última epidemia gripal una emergencia de las bronquiolitis causadas por el virus influenza A en la población pediátrica. Esta observación debería plantearnos una doble estrategia; por un lado de tipo profiláctico (vacunación de madres gestantes) y, por otro, de tipo diagnóstico (detección antigénica rápida del virus influenza A) (AU)
