ABSTRACT
The action of a low volume (one drop) of sodium carboxymethylcellulose (CMC)-papain (PP) association gel to prevent peritoneal adhesions were studied in female Wistar rats. After ether anesthesia and a midline laparotomy incision, the right parietal peritoneum was pinched with a fine hemostat and the pinched peritoneal fold was then ligated. This maneuver was repeated thrice creating four points as if they were little "polyps" with a standardized size. Before closing the incision it was deposited on each point 0.05 ml (one drop) of CMC 2% (group A) or CMC + PP 0.4% (group B), with a total volume of 0.2 ml. These groups were compared with another similar group (group C) of a previous research, in which was used 7 ml/kg of body weight of CMC + PP 0.4% (1.5 ml by animal). Statistically significance was not noticed between groups A and B but it was noticed between these two groups (A and B) and group C (p < 0.001 and p < 0.01, respectively). It was concluded that the lowest effective volume (between 0.2 and 1.5 ml/animal) which can allow the desired effect is to be determined in order to diminish the quantity of substance to be deposited in the abdominal cavity.
Subject(s)
Carboxymethylcellulose Sodium/administration & dosage , Papain/administration & dosage , Peritoneal Diseases/prevention & control , Animals , Dose-Response Relationship, Drug , Drug Therapy, Combination , Female , Laparotomy , Peritoneal Diseases/pathology , Rats , Rats, Inbred Strains , Tissue Adhesions/pathology , Tissue Adhesions/prevention & controlABSTRACT
Os autores apresentam um estudo experimental realizado com ratos Wistar, com intuito de comparar a ocorrência de aderências peritoneais após estimulaçao por quatro tipos de lesoes da parede abdominal em sua face peritoneal. Vinte animais machos foram utilizados após laparatomia mediana de aproximadamente 6 cm de extensao e incluídos em um dos quatro grupos a seguir; Grupo A - áreas isquêmicas; Grupo B - áreas isquêmicas cruentas; Grupo C - áreas cruentas; Grupo D - áreas cruentas cauterizadas por bisturi elétrico. Observou-se que o Grupo C mostrou diferença estatisticamente significante em relaçao aos outros grupos (A, B e D), mostrando um menor índice de aderências.
Subject(s)
Animals , Male , Rats , Peritoneal Diseases/etiology , Laparotomy , Postoperative Complications , Rats, Wistar , Tissue Adhesions/etiologyABSTRACT
The inhibitory action of sodium carboxymethylcellulose (CMC) and papain (PP) in peritoneal adhesions formation, were studied in Wistar rats. After ether anesthesia and a midline laparotomy incision, the right parietal peritoneum was pinched with a fine hemostat and the pinched peritoneal fold was then ligated. This maneuver was repeated thrice creating four point as if they were little "polyps" with a standardized size. Before closing the incision, it was deposited on the four points of each animal the following substances: group A--saline solution; group B--CMC 2%; group C--PP 0.4% in distilled water; group D--CMC 2% + PP 0.2%; group E--CMC 2% + PP 0.4%. After ten days, the rats were sacrificed and classified according to the number of "points" that showed adhesions with the abdominal structures. There was a statistically significant decrease in the incidence of adhesions only in the group E (CMC + PP 0.4%).
