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1.
Int Immunopharmacol ; 73: 435-441, 2019 Aug.
Article in English | MEDLINE | ID: mdl-31154288

ABSTRACT

Studies suggest that hydrogen sulfide (H2S) plays a relevant and beneficial role in the pathophysiology of pulmonary allergic diseases, such as asthma. These diseases may be triggered by changes in airway epithelium caused by repeated exposure to environmental allergens. This study aimed to investigate whether H2S protects against bronchial epithelium apoptosis in allergic inflammation in mice. The effects of H2S on the production of Th2 cytokines and on the infiltration of pulmonary inflammatory cells were also studied. Female BALB/c mice previously sensitized with ovalbumin (OVA) were treated with H2S donor (sodium hydrosulfide [NaHS]) 30 min prior to OVA challenge. After euthanasia (48 h post challenge), the right lung was homogenized to study apoptosis protein expression and to analyze cytokine levels in lung tissue. The left lobe was fixed in formalin for morphological analysis of lung tissue and verification of apoptosis in situ by the TUNEL assay. Histological results showed that NaHS reduced the airway inflammatory infiltrate and prevented an increase in the IL-4, IL-5 and IL-25 levels caused by OVA challenge. Activation of caspase 3 and FasL in response to the allergen was also fully prevented by NaHS treatment. TUNEL staining showed that the challenge from OVA significantly increased the rate of apoptosis in the bronchiolar epithelium, and that this incremental apoptosis was abolished by NaHS treatment. In conclusion, our results showed that H2S donor has a protective effect against airway epithelium damage caused by an allergic reaction, and represents a potential agent in treating allergic lung disorders, such as asthma.


Subject(s)
Cytokines/immunology , Epithelium/drug effects , Lung/drug effects , Respiratory Hypersensitivity/immunology , Animals , Apoptosis/drug effects , Disease Models, Animal , Epithelium/immunology , Epithelium/pathology , Female , Hydrogen Sulfide , Lung/immunology , Lung/pathology , Mice, Inbred BALB C , Ovalbumin , Respiratory Hypersensitivity/pathology , Sulfides/pharmacology
2.
Int Immunopharmacol ; 39: 57-62, 2016 Oct.
Article in English | MEDLINE | ID: mdl-27424079

ABSTRACT

OBJECTIVE: The interaction between nitric oxide (NO) and hydrogen sulfide (H2S) in the airways could have significant implications for the pathogenesis and therapeutic effects of both on lung diseases. In this study we investigated whether the beneficial effects of H2S on asthma could be comparable to that inhibition of inducible NO synthase (iNOS). METHODS: Female BALB/C mice sensitized with ovalbumin (OVA) received either the H2S donor sodium hydrosulfide (NaHS, 14µmol/kg) or the iNOS inhibitor 1400W (1mg/kg), 30min before each OVA challenge during six days. On the first, second and sixth days, the leucocyte infiltration in lung parenchyma and bronchoalveolar lavage was evaluated. The aconitase activity (a sensor of O2 formation) and lipid peroxidation, as well as levels of reduced glutathione (GSH) and oxidized glutathione (GSSG) were determined in the lung tissues. RESULTS: OVA-challenge caused a significant and time-dependent increase in the eosinophil number in the airways, which was accompanied by a significant decrease of aconitase activity and GSH/GSSG ratio along with enhanced lipid peroxidation in the lungs. Treatment with NaHS or 1400W significantly attenuated the airways eosinophilia that was paralleled by an increase in aconitase activity and decrease of lipid peroxidation. NaHS or 1400W treatments also reversed the decreased GSH/GSSG ratio seen after OVA-challenge. CONCLUSIONS: The present study shows for the first time that the increased GSH/GSSG ratio caused by either H2S supplementation or iNOS-inhibition is a potential mechanism protecting airways against oxidative stress and inflammatory lung diseases.


Subject(s)
Asthma/drug therapy , Enzyme Inhibitors/therapeutic use , Glutathione/metabolism , Hydrogen Sulfide/therapeutic use , Lung/drug effects , Neutrophils/drug effects , Pneumonia/drug therapy , Aconitate Hydratase/metabolism , Animals , Cell Movement/drug effects , Enzyme Inhibitors/pharmacology , Female , Lung/immunology , Lung/pathology , Mice , Mice, Inbred BALB C , Neutrophils/immunology , Nitric Oxide Synthase Type II/antagonists & inhibitors , Oxidative Stress/drug effects
3.
Arch Toxicol ; 88(8): 1589-605, 2014 Aug.
Article in English | MEDLINE | ID: mdl-24554396

ABSTRACT

High diesel exhaust particle levels are associated with increased health effects; however, knowledge on the impact of its chemical contaminant 1,2-naphthoquinone (1,2-NQ) is limited. We investigated whether postnatal and adult exposures to 1,2-NQ influence allergic reaction and the roles of innate and adaptive immunity. Male neonate (6 days) and adult (56 days) C57Bl/6 mice were exposed to 1,2-NQ (100 nM; 15 min) for 3 days, and on day 59, they were sensitized and later challenged with ovalbumin (OVA). Airway hyper-responsiveness (AHR) and production of cytokines, immunoglobulin E (IgE) and leukotriene B4 (LTB4) were measured in the airways. Postnatal exposure to 1,2-NQ activated dendritic cells in splenocytes by increasing expressing cell surface molecules (e.g., CD11c). Co-exposure to OVA effectively polarized T helper (Th) type 2 (Th2) by secreting Th2-mediated cytokines. Re-stimulation with unspecific stimuli (PMA and ionomycin) generated a mixed Th1 (CD4(+)/IFN-γ(+)) and Th17 (CD4(+)/IL-17(+)) phenotype in comparison with the vehicle-matched group. Postnatal exposure to 1,2-NQ did not induce eosinophilia in the airways at adulthood, although it evoked neutrophilia and exacerbated OVA-induced eosinophilia, Th2 cytokines, IgE and LTB4 production without affecting AHR and mast cell degranulation. At adulthood, 1,2-NQ exposure evoked neutrophilia and increased Th1/Th2 cytokine levels, but failed to affect OVA-induced eosinophilia. In conclusion, postnatal exposure to 1,2-NQ increases the susceptibility to antigen-induced asthma. The mechanism appears to be dependent on increased expression of co-stimulatory molecules, which leads to cell presentation amplification, Th2 polarization and enhanced LTB4, humoral response and Th1/Th2 cytokines. These findings may be useful for future investigations on treatments focused on pulmonary illnesses observed in children living in heavy polluted areas.


Subject(s)
Aging/immunology , Air Pollutants/toxicity , Inhalation Exposure/adverse effects , Naphthoquinones/toxicity , Pneumonia/chemically induced , Respiratory Hypersensitivity/chemically induced , Vehicle Emissions/toxicity , Adaptive Immunity/drug effects , Aging/drug effects , Animals , Animals, Newborn , Cytokines/immunology , Disease Susceptibility/chemically induced , Immunity, Innate/drug effects , Immunoglobulin E/immunology , Inhalation Exposure/analysis , Leukotriene B4/immunology , Male , Ovalbumin/immunology , Pneumonia/immunology , Respiratory Hypersensitivity/immunology , Vehicle Emissions/analysis
4.
Eur J Pharmacol ; 698(1-3): 463-9, 2013 Jan 05.
Article in English | MEDLINE | ID: mdl-23183109

ABSTRACT

Recent studies show that endogenous hydrogen sulfide (H(2)S) plays an anti-inflammatory role in the pathogenesis of airway inflammation. This study investigated whether exogenous H(2)S may counteract oxidative stress-mediated lung damage in allergic mice. Female BALB/c mice previously sensitized with ovalbumin (OVA) were treated with sodium hydrosulfide (NaHS) 30 min before OVA challenge. Forty eight hours after antigen-challenge, the mice were killed and leukocyte counting as well as nitrite plus nitrate concentrations were determined in the bronchoalveolar lavage fluid, and lung tissue was analysed for nitric oxide synthase (NOS) activity, iNOS expression, superoxide dismutase (SOD), catalase, glutathione reductase (GR) and glutathione peroxidase (GPx) activities, thiobarbituric acid reactive species and 3-nitrotyrosine containing proteins (3-NT). Pre-treatment of OVA-sensitized mice with NaHS resulted in significant reduction of both eosinophil and neutrophil migration to the lungs, and prevented the elevation of iNOS expression and activity observed in the lungs from the untreated allergic mice, although it did not affect 3-NT. NaHS treatment also abolished the increased lipid peroxidation present in the allergic mouse lungs and increased SOD, GPx and GR enzyme activities. These results show, for the first time, that the beneficial in vivo effects of the H(2)S-donor NaHS on allergic airway inflammation involve its inhibitory action on leukocyte recruitment and the prevention of lung damage by increasing endogenous antioxidant defenses. Thus, exogenous administration of H(2)S donors may be beneficial in reducing the deleterius impact of allergic pulmonary disease, and might represent an additional class of pharmacological agents for treatment of chronic pulmonary diseases.


Subject(s)
Hydrogen Sulfide/pharmacology , Hypersensitivity/metabolism , Lung/drug effects , Lung/metabolism , Oxidative Stress/drug effects , Animals , Catalase/metabolism , Female , Glutathione Peroxidase/metabolism , Glutathione Reductase/metabolism , Hypersensitivity/enzymology , Hypersensitivity/immunology , Leukocytes/drug effects , Leukocytes/immunology , Lung/enzymology , Lung/immunology , Mice , Mice, Inbred BALB C , Nitric Oxide Synthase/metabolism , Sulfides/pharmacology , Superoxide Dismutase/metabolism , Thiobarbiturates/metabolism , Tyrosine/analogs & derivatives , Tyrosine/metabolism
5.
Eur J Pharmacol ; 566(1-3): 200-5, 2007 Jul 02.
Article in English | MEDLINE | ID: mdl-17368616

ABSTRACT

This study was designed to understand the relationship between interleukin-5 and eotaxin in modulating the chemotaxis of eosinophils obtained from healthy subjects and subjects with allergic rhinitis. Chemotaxis of eosinophils from patients with allergic rhinitis toward interleukin-5 (0.25 ng/ml) was 78% higher than that of healthy subjects. Incubation of eosinophils with eotaxin (100 ng/ml) did not change the interleukin-5-induced chemotaxis of eosinophils from healthy subjects, but it reversed the enhanced chemotaxis seen in eosinophils from allergic patients. Chemotaxis of eosinophils from patients with allergic rhinitis toward eotaxin (100 ng/ml) was 65% higher than that of eosinophils from healthy subjects. Incubation of eosinophils with interleukin-5 (100 ng/ml) significantly increased the eotaxin-induced chemotaxis in both subject groups, but such increases were markedly higher for cells from patients with allergic rhinitis. Our finding that eotaxin inhibits the enhanced eosinophil chemotaxis toward interleukin-5 in primed cells suggests that this chemokine may downregulate eosinophil accumulation in the nasal mucosa of allergic patients.


Subject(s)
Chemokines, CC/pharmacology , Eosinophils/drug effects , Interleukin-5/pharmacology , Rhinitis/immunology , Cell Adhesion/drug effects , Chemokine CCL11 , Chemotaxis, Leukocyte/drug effects , Eosinophils/immunology , Humans , Interleukin-5/blood , Rhinitis/blood
6.
Leuk Res ; 31(5): 695-7, 2007 May.
Article in English | MEDLINE | ID: mdl-16956660

ABSTRACT

Migration of eosinophil (Eo) into tissues is a hallmark of chronic eosinophilic leukaemia (CEL), but the exact mechanism involved in cell migration is unknown. We report on a patient with CEL who presented high expressions of VLA-4, LFA-1 and Mac-1 integrins on the Eo surface, increased chemotaxis of Eo to eotaxin, decreased chemotaxis of Eo after inhibition of the cyclic guanosine monophosphate (cGMP), and a high level of intracellular cGMP. These findings suggest that an upregulation of expression of these integrins on Eo surface and a high intracellular level of cGMP may be involved in the increased Eo migration observed in our CEL patient. However, the definitive roles of these molecules in the proliferation and migration of Eo in CEL disease require wider confirmation by analysis of additional patients with the disease.


Subject(s)
Cell Movement , Eosinophils/metabolism , Hypereosinophilic Syndrome/metabolism , Integrin alpha4beta1/metabolism , Lymphocyte Function-Associated Antigen-1/metabolism , Macrophage-1 Antigen/metabolism , Chemotactic Factors, Eosinophil , Chemotaxis, Leukocyte , Chronic Disease , Eosinophils/pathology , Female , Humans , Hypereosinophilic Syndrome/diagnosis , Hypereosinophilic Syndrome/therapy , Middle Aged
7.
Biochem Pharmacol ; 66(1): 43-50, 2003 Jul 01.
Article in English | MEDLINE | ID: mdl-12818364

ABSTRACT

Recent research demonstrates that the beta1 integrins may be involved in neutrophil migration. Here, we investigate the role of nitric oxide in the expression and function of the very late antigen-4 (VLA-4) and Mac-1 integrins on human neutrophils. Human blood neutrophils were treated with N(omega)-nitro-L-arginine methyl ester (L-NAME) and their adhesion to fibronectin (FN) and serum observed. Adhesion of neutrophils to FN and serum increased significantly following incubation with 0.1mM L-NAME by 65.5 and 44.6%, respectively. Increased adhesions to FN and serum were abolished by a VLA-4-specific monoclonal antibody, HP2/1, and a Mac-1-specific monoclonal antibody, ICRF 44, respectively. The microfilament- and microtubule-depolymerizing agents, dihydrochalasin B and nocodazole, were also able to reverse L-NAME-induced adhesion to both FN and serum. L-NAME induced a discrete increase in the expression of CD49d (VLA-4, 25.3+/-4.8%), but not CD11b, on the neutrophil cell surface, as detected by flow cytometry. Results indicate that NO has a role in regulating VLA-4 and Mac-1 function on the human neutrophil cell surface and that this modulation in integrin function is accompanied by cytoskeletal rearrangements and changes in the ability of the neutrophil to adhere to the extracellular matrix.


Subject(s)
Integrin alpha4beta1/biosynthesis , Neutrophils/metabolism , Nitric Oxide/physiology , Antibodies, Monoclonal/pharmacology , Cell Adhesion/drug effects , Cell Adhesion Molecules/metabolism , Cells, Cultured , Drug Interactions , Enzyme Inhibitors/pharmacology , Fibronectins/metabolism , Guanylate Cyclase/antagonists & inhibitors , Humans , Integrin alpha4beta1/immunology , Integrins/biosynthesis , Integrins/immunology , Macrophage-1 Antigen/immunology , Microtubules/drug effects , NG-Nitroarginine Methyl Ester/pharmacology , Neutrophils/drug effects , Oxadiazoles/pharmacology , Quinoxalines/pharmacology
8.
Eur J Pharmacol ; 442(1-2): 155-62, 2002 May 03.
Article in English | MEDLINE | ID: mdl-12020693

ABSTRACT

The expression of nitric oxide (NO) synthases and the role of the NO cyclic GMP pathway on the migration of eosinophils from untreated patients with allergic rhinitis were investigated. Inducible NO synthase was strongly expressed in eosinophils from healthy individuals, but not in eosinophils from allergic rhinitis patients. The neuronal isoform was observed in eosinophils from each group studied, whereas no staining for the endothelial isoform was detected in either group. The chemotaxis to N-formyl-methionyl-leucyl-phenylalanine (fMLP, 5 x 10(-7) M) and eotaxin (100 ng/ml) was significantly potentiated in allergic rhinitis eosinophils. In both groups, N(omega)-nitro-L-arginine methyl ester (L-NAME, 1.0 mM) or 1H(1,2,4)-oxadiazolo(4,3,-a)quinoxalin-1-one (ODQ, 0.2 mM) markedly reduced the chemotaxis. The selective iNOS inhibitor N-(3-(aminomethyl)benzyl)acetamidine (1400 W, 0.1-1.0 mM) significantly reduced the chemotaxis of eosinophils from healthy but not from allergic rhinitis subjects. The inhibition by L-NAME was restored by 3-morpholinosydnonimine (SIN-1) and S-nitroso-N-acetyl-penicillamine, whereas the inhibition by ODQ was restored by dibutyryl cyclic GMP. In conclusion, both endothelial and inducible NO synthase isoforms are absent in allergic rhinitis eosinophils, suggesting that the NO cyclic GMP pathway in this cell type is maintained through the activity of a neuronal isoform.


Subject(s)
Eosinophils/enzymology , Nitric Oxide Synthase/biosynthesis , Rhinitis, Allergic, Perennial/blood , Cell Movement/drug effects , Chemokine CCL11 , Chemokines, CC/pharmacology , Chemotaxis/drug effects , Dose-Response Relationship, Drug , Enzyme Inhibitors/pharmacology , Eosinophils/drug effects , Eosinophils/pathology , Guanylate Cyclase/antagonists & inhibitors , Guanylate Cyclase/metabolism , Humans , Isoenzymes/biosynthesis , Molsidomine/analogs & derivatives , Molsidomine/pharmacology , N-Formylmethionine Leucyl-Phenylalanine/pharmacology , NG-Nitroarginine Methyl Ester/pharmacology , Nitric Oxide/metabolism , Nitric Oxide Donors/pharmacology , Nitric Oxide Synthase/antagonists & inhibitors , Nitric Oxide Synthase Type II , Oxadiazoles/pharmacology , Quinoxalines/pharmacology , Rhinitis, Allergic, Perennial/pathology , S-Nitroso-N-Acetylpenicillamine/pharmacology , Solubility
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