ABSTRACT
This study aimed to assess the ultrapure cannabidiol (CBD) antibacterial activity and to investigate the antibacterial activity of the combination CBD + polymyxin B (PB) against Gram-negative (GN) bacteria, including PB-resistant Gram-negative bacilli (GNB). We used the standard broth microdilution method, checkerboard assay, and time-kill assay. CBD exhibited antibacterial activity against Gram-positive bacteria, lipooligosaccharide (LOS)-expressing GN diplococcus (GND) (Neisseria gonorrhoeae, Neisseria meningitidis, Moraxella catarrhalis), and Mycobacterium tuberculosis, but not against GNB. For most of the GNB studied, our results showed that low concentrations of PB (≤ 2 µg/mL) allow CBD (≤ 4 µg/mL) to exert antibacterial activity against GNB (e.g., Klebsiella pneumoniae, Escherichia coli, Acinetobacter baumannii), including PB-resistant GNB. CBD + PB also showed additive and/or synergistic effect against LOS-expressing GND. Time-kill assays results showed that the combination CBD + PB leads to a greater reduction in the number of colony forming units per milliliter compared to CBD and PB alone, at the same concentration used in combination, and the combination CBD + PB was synergistic for all four PB-resistant K. pneumoniae isolates evaluated. Our results show that CBD has translational potential and should be further explored as a repurposed antibacterial agent in clinical trials. The antibacterial efficacy of the combination CBD + PB against multidrug-resistant and extensively drug-resistant GNB, especially PB-resistant K. pneumoniae, is particularly promising.
Subject(s)
Cannabidiol , Polymyxin B , Anti-Bacterial Agents/pharmacology , Cannabidiol/pharmacology , Drug Repositioning , Drug Resistance, Multiple, Bacterial , Drug Synergism , Gram-Negative Bacteria , Klebsiella pneumoniae , Microbial Sensitivity Tests , Polymyxin B/pharmacologyABSTRACT
Leukotriene B4 (LTB4) is essential for host immune defence. It increases neutrophil recruitment, phagocytosis and pathogen clearance, and decreases oedema and inflammasome activation. The host response and the role of LTB4 during Achromobacter xylosoxidans infection remain unexplored. Wild-type (129sv) and LTB4 deficient (Alox5 -/-) mice were intratracheally infected with A. xylosoxidans. Wild-type 129sv infected mice survived beyond the 8th day post-infection, exhibited increased levels of LTB4 in the lung on the 1st day, while levels of PGE2 increased on the 7th day post-infection. Infected Alox5 -/- mice showed impaired bacterial clearance, increased lung inflammation, and succumbed to the infection by the 7th day. We found that exogenous LTB4 does not affect the phagocytosis of A. xylosoxidans by alveolar macrophages in vitro. However, treatment of infected animals with LTB4 protected from mortality, by reducing the bacterial load and inflammation via BLT1 signalling, the high affinity receptor for LTB4. Of importance, we uncovered that LTB4 induces gene and protein expression of α-defensin-1 during the infection. This molecule is essential for bacterial clearance and exhibits potent antimicrobial activity by disrupting A. xylosoxidans cell wall. Taken together, our data demonstrate a major role for LTB4 on the control of A. xylosoxidans infection.
Subject(s)
Achromobacter denitrificans/physiology , Gram-Negative Bacterial Infections/immunology , Inflammation/immunology , Leukotriene B4/metabolism , Lung/immunology , Macrophages, Alveolar/immunology , 5-Lipoxygenase-Activating Proteins/genetics , Animals , Bacterial Load , Cells, Cultured , Dinoprostone/metabolism , Mice , Mice, 129 Strain , Mice, Knockout , Phagocytosis , Receptors, Leukotriene B4/metabolism , Signal Transduction , alpha-Defensins/metabolismABSTRACT
Cryptococcus laurentii é um patógeno humano raro, ubíquo na natureza. O objetivo deste estudo foi avaliar a aplicação do método de difusão do disco para determinar a sensibilidade ao fluconazol de isolados de C. laurentii e determinar a concentração fungicida mínima (CFM) do fluconazol. Foi determinada a sensibilidade ao fluconazol pelos métodos de difusão do disco e microdiluição em caldo de 11 isolados de C. laurentii, de acordo com CLSI (Clinical and Laboratory StandardsInstitute), e a CFM pelo método de microdiluição em caldo. O método de difusão do disco mostrou quatro isolados sensíveis, três sensíveis dose-dependentes e quatro resistentes, enquanto que pelo método de microdiluição, 10 isolados foram sensíveis e um sensível dose-dependente. A concordância entre os dois métodos foi de 36,4por cento. Um isolado apresentou CFM de 8 mi g/mL e dois de 64 mi g/mL. Embora o número de isolados estudados seja pequeno, os resultados sugeremque o método de difusão do disco não deve ser usado na determinação da sensibilidade in vitro dos isolados de C. laurentii ao fluconazol, e apesar de ser uma droga fungistática, o fluconazol pode apresentar atividade fungicida in vitro para alguns isolados do complexo C. laurentii.
Subject(s)
Cryptococcus/isolation & purification , Fluconazole/pharmacology , Disk Diffusion Antimicrobial TestsABSTRACT
The pathogenicity of Cryptococcus neoformans is heterogeneous and is associated with the expression of virulence factors. This study aimed to correlate the pathogenicity of C. neoformans var. grubii in BALB/c mice with in vitro virulence factors, fluconazole minimal inhibitory concentrations (MICs) and molecular profiles, before and after animal passage. Ten environmental isolates and one ATCC strain of C. neoformans var. grubii mating type α were evaluated. Most isolates (91 percent) killed 50 percent or more of the infected animals by day 24 postinfection and were recovered from the lungs and brains of surviving animals on days 7 and 14 postinfection. The burden of yeast in the lungs was more variable than that in the brain. The differences in the expression of virulence factors (growth at 37ºC, presence and size of the capsule and production of melanin, urease, proteinase and phospholipase) by most isolates pre and postpassage in animals were not statistically significant. The fluconazole MICs in postpassaged lines differed by a one-dilution from the MIC of the corresponding prepassaged line for six isolates. Using molecular typing [polymerase chain reaction-fingerprinting with (GACA)4 and M13], eight isolates were identified as VNI and three as VNII. We concluded that different isolates with the same molecular and phenotypic profiles, including isolates that are markedly hypervirulent, span a wide range of virulence and there were no changes in virulence factors in the postpassaged lines when compared with the corresponding nonpassaged lines.
Subject(s)
Animals , Female , Mice , Cryptococcus neoformans , Cryptococcus neoformans , Fluconazole , Molecular Typing/methods , Virulence Factors , Cryptococcus neoformans , DNA Fingerprinting , DNA, Fungal , Microbial Sensitivity Tests , Mycological Typing Techniques , Polymerase Chain Reaction , Time FactorsABSTRACT
The pathogenicity of Cryptococcus neoformans is heterogeneous and is associated with the expression of virulence factors. This study aimed to correlate the pathogenicity of C. neoformans var. grubii in BALB/c mice with in vitro virulence factors, fluconazole minimal inhibitory concentrations (MICs) and molecular profiles, before and after animal passage. Ten environmental isolates and one ATCC strain of C. neoformans var. grubii mating type α were evaluated. Most isolates (91%) killed 50% or more of the infected animals by day 24 postinfection and were recovered from the lungs and brains of surviving animals on days 7 and 14 postinfection. The burden of yeast in the lungs was more variable than that in the brain. The differences in the expression of virulence factors (growth at 37ºC, presence and size of the capsule and production of melanin, urease, proteinase and phospholipase) by most isolates pre and postpassage in animals were not statistically significant. The fluconazole MICs in postpassaged lines differed by a one-dilution from the MIC of the corresponding prepassaged line for six isolates. Using molecular typing [polymerase chain reaction-fingerprinting with (GACA)4 and M13], eight isolates were identified as VNI and three as VNII. We concluded that different isolates with the same molecular and phenotypic profiles, including isolates that are markedly hypervirulent, span a wide range of virulence and there were no changes in virulence factors in the postpassaged lines when compared with the corresponding nonpassaged lines.
Subject(s)
Cryptococcus neoformans/drug effects , Cryptococcus neoformans/pathogenicity , Fluconazole/pharmacology , Molecular Typing/methods , Virulence Factors/analysis , Animals , Cryptococcus neoformans/genetics , DNA Fingerprinting , DNA, Fungal/analysis , Female , Mice , Microbial Sensitivity Tests , Mycological Typing Techniques , Polymerase Chain Reaction , Time FactorsABSTRACT
Yeasts of the Cryptococcus genus are distributed in nature associated to animal and vegetal organic residues. Occasionally, species other than C. neoformans may be responsible for infectious diseases in human and animals. This study aims to determine the occurrence of Cryptococcus species in the atmosphere and bird droppings in the city of Ribeirão Preto, São Paulo, Brazil, and to evaluate three virulence factors: capsule formation, growth at 37 degrees C and melanin production. We analyzed 86 environmental samples (54 droppings and 32 air). Of the 41 strains isolated, 15 were C. neoformans var. neoformans (12 droppings and 3 air), 15 C. albidus (12 droppings and 3 air), 9 C. laurentii (7 droppings and 2 air) and 2 C. uniguttulatus (from droppings). Capsules were produced by 93.3% of C. neoformans var. neoformans, 66.7% of C. albidus, 88.9% of C. laurentii and 50% (1/2) of C. uniguttulatus. All strains of C. neoformans, 20% of C. albidus and 44.4% of C. laurentii were able to grow at 37 degrees C. The melanin production on DOPA agar was verified in C. neoformans (93.3%), C. albidus (26.7%) and C. laurentii (66.7%). We concluded that different Cryptococcus species coexist in the same ecological niche and they are able to produce virulence factors.
Subject(s)
Cryptococcus/isolation & purification , Cryptococcus/metabolism , Environmental Microbiology , Feces/microbiology , Virulence Factors/metabolism , Animals , Bacterial Capsules/metabolism , Birds/microbiology , Brazil , Cryptococcus/classification , Melanins/metabolismABSTRACT
The present study was designed to determine the prevalence and extended-spectrum beta-lactamase (ESBL) types in clinical isolates of Klebsiella spp. at a university hospital located in the Brazilian southern region (Ribeirão Preto, São Paulo) as well as their antibiotic susceptibility and genetic profiles. This study included 147 non-repeat Klebsiella spp. isolates collected from January to June 2000, of which 23 K. pneumoniae and 8 K. oxytoca were selected as ESBL producers by using the Oxoid combination disk method and Etest ESBL strip. beta-lactamases were characterized by IEF, PCR and sequencing assays using primers for ESBL genes. Antibiotic susceptibility was evaluated by MicroScan system. Dissemination of two major clones of ESBL-producing Klebsiella spp. occurred in the hospital. According to the results obtained in this study there was a clonal spread of CTX-M-producing K. oxytoca in five clinics and dissemination of ESBL-producing K. pneumoniae in the nursery and pediatrics wards.
Subject(s)
Drug Resistance, Multiple, Bacterial , Klebsiella Infections/epidemiology , Klebsiella/enzymology , Klebsiella/isolation & purification , beta-Lactamases/metabolism , Anti-Bacterial Agents/pharmacology , Bacterial Proteins/analysis , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Brazil/epidemiology , Hospitals, University , Humans , Klebsiella/drug effects , Klebsiella/genetics , Klebsiella Infections/microbiology , Klebsiella Infections/transmission , Microbial Sensitivity Tests , beta-Lactamases/analysis , beta-Lactamases/geneticsABSTRACT
PFGE (pulsed field gel eletrophoresis) é a sigla usada para indicar qualquer técnica de eletroforese apropriada para separar grandes fragmentos de DNA, por meio da reorientação do DNA em gel pela ação de campos elétricos alternados. Esta técnica é reconhecida como padrão ouro para identificação de linhagens bacterianas, fúngicas e de protozoários. O principal objetivo deste estudo de revisão é o da elucidação dos fundamentos da técnica de PFGE ou eletroforese de campo pulsante aplicada em estudo com bactéria. Sua resolução depende de uma série de fatores como: voltagem, concentração de agarose, temperatura, solução tamponante, tempo de pulso e de corrida eletroforética. A compreensão dos mecanismos moleculares envolvidos nesse tipo de eletroforese é fundamental para a otimização e obtenção dos resultados apropriados.
Subject(s)
DNA, Bacterial , Electrophoresis, Gel, Pulsed-Field , Molecular Epidemiology , Bacteriological Techniques , GenotypeABSTRACT
The present study evaluated in vitro susceptibility testing of dermatophytes isolates from healthy cattle and soil samples against three antifungal agents and three topical veterinarian drugs. Itraconazole and terbinafine showed a higher in vitro fungicidal activity than fluconazole. The veterinarian drugs LEPECID and iodine 5% were more active in vitro than the UNGUENTO spray. All drugs showed fungicidal activity against Microsporum gypseum, and they may be considered as efficient agents for the topical treatment of dermatophytoses in cattle.
