Improving Sphenophorus levis Adult Mortality Through Solid Insecticide Applications and Increased Insecticide Dose.

The sugarcane weevil (Sphenophorus levis Vaurie, 1978) is currently considered the most important sugarcane pest in Brazil, causing significant yield losses. Application methods of insecticides for S. levis control have not been effective, mostly due to the insect's cryptic behavior below the soil surface which suppresses the correct placement of insecticide active ingredients on target. Two experiments were conducted using an innovative bioassay methodology that simulates sugarcane field conditions to effectively evaluate S. levis adult mortality and insecticide residues in the soil under different treatments. The first experiment aimed to assess the efficacy of two liquid- and solid-applied insecticides, while the second aimed to examine the effect of increasing the dose of lambda-cyhalothrin + thiamethoxam on S. levis adult control. The novel bioassays simulated liquid and solid insecticide applications on sugarcane by exposing S. levis adults to chemical residuals on rhizomes and in soil after insecticide application. In the first experiment, low S. levis adult control was detected (< 53% mortality) across all treatments, where both solid and liquid applications of lambda-cyhalothrin + thiamethoxam provided greater efficacy levels than imidacloprid and control treatments, respectively. Solid applications maintained higher insecticide concentrations in the soil for longer periods than liquid insecticide applications, providing maximum insect control levels 7 days after application. The second experiment revealed that solid applications at higher insecticide doses significantly improved control of S. levis adult (76.7% mortality) and resulted in greater insecticide concentrations in the soil compared to the recommended label rate (58.8% mortality).

Analysis of peri-implant bone tissue between hydrophilic and rough implant surfaces in spontaneously hypertensive rats treated with losartan.

OBJECTIVES: to evaluate the morphological and functional characteristics of the peri-implant bone tissue that was formed during the healing process by the placement implants using two different surface treatments: hydrophilic Acqua™ (ACQ) and rough NeoPoros™ (NEO), in spontaneously hypertensive (SHR) and normotensive rats (Wistar) whether or not treated with losartan. METHODOLOGY: In total, 96 male rats (48 Wistar and 48 SHR) were divided into eight subgroups: absolute control rough (COA NEO), absolute control hydrophilic (COA ACQ), losartan control rough (COL NEO), losartan control hydrophilic (COL ACQ), SHR absolute rough (SHR NEO), SHR absolute hydrophilic (SHR ACQ), SHR losartan rough (SHRL NEO), and SHR losartan hydrophilic (SHRL ACQ). The rats medicated with losartan received daily doses of the medication. NeoPoros™ and Acqua™ implants were installed in the tibiae of the rats. After 14 and 42 days of the surgery, the fluorochromes calcein and alizarin were injected in the rats. The animals were euthanized 67 days after treatment. The collected samples were analyzed by immunohistochemistry, biomechanics, microcomputerized tomography, and laser confocal scanning microscopy analysis. RESULTS: The osteocalcin (OC) and vascular endothelium growth factor (VEGF) proteins had moderate expression in the SHRL ACQ subgroup. The same subgroup also had the highest implant removal torque. Regarding microarchitectural characteristics, a greater number of trabeculae was noted in the control animals that were treated with losartan. In the bone mineralization activity, it was observed that the Acqua™ surface triggered higher values of MAR (mineral apposition rate) in the COA, COL, and SHRL groups (p<0.05). CONCLUSION: the two implant surface types showed similar responses regarding the characteristics of the peri-implant bone tissue, even though the ACQ surface seems to improve the early stages of osseointegration.

Analysis of peri-implant bone tissue between hydrophilic and rough implant surfaces in spontaneously hypertensive rats treated with losartan

Abstract Hypertensive individuals present alterations in their calcium metabolism that consequently decrease the concentration of this mineral in the bone tissue. Objectives to evaluate the morphological and functional characteristics of the peri-implant bone tissue that was formed during the healing process by the placement implants using two different surface treatments: hydrophilic Acqua™ (ACQ) and rough NeoPoros™ (NEO), in spontaneously hypertensive (SHR) and normotensive rats (Wistar) whether or not treated with losartan. Methodology In total, 96 male rats (48 Wistar and 48 SHR) were divided into eight subgroups: absolute control rough (COA NEO), absolute control hydrophilic (COA ACQ), losartan control rough (COL NEO), losartan control hydrophilic (COL ACQ), SHR absolute rough (SHR NEO), SHR absolute hydrophilic (SHR ACQ), SHR losartan rough (SHRL NEO), and SHR losartan hydrophilic (SHRL ACQ). The rats medicated with losartan received daily doses of the medication. NeoPoros™ and Acqua™ implants were installed in the tibiae of the rats. After 14 and 42 days of the surgery, the fluorochromes calcein and alizarin were injected in the rats. The animals were euthanized 67 days after treatment. The collected samples were analyzed by immunohistochemistry, biomechanics, microcomputerized tomography, and laser confocal scanning microscopy analysis. Results The osteocalcin (OC) and vascular endothelium growth factor (VEGF) proteins had moderate expression in the SHRL ACQ subgroup. The same subgroup also had the highest implant removal torque. Regarding microarchitectural characteristics, a greater number of trabeculae was noted in the control animals that were treated with losartan. In the bone mineralization activity, it was observed that the Acqua™ surface triggered higher values of MAR (mineral apposition rate) in the COA, COL, and SHRL groups (p<0.05). Conclusion the two implant surface types showed similar responses regarding the characteristics of the peri-implant bone tissue, even though the ACQ surface seems to improve the early stages of osseointegration.

Stomatological profile of elderly patients who received dental care at a reference Hospital of Belo Horizonte, Minas Gerais - Brazil: a cross-sectional study

Aim: The aim of this study was to draw a stomatological profile of elderly patients treated at the Stomatology Service of a referral hospital in the city of Belo Horizonte, Minas Gerais ­ Brazil.Methods: Information was collected on the diagnostic hypotheses and definitive diagnoses of the elderly (aged 60 to 98 years) treated at the service, from September 2014 to December 2017. Results: Medical records of 1388 patients were evaluated, and 281 different diagnostic hypotheses were found. For males, there was a higher prevalence in both diagnostic and definitive diagnoses for benign and malignant epithelial neoplasms; In females, there was a higher prevalence, also in the hypotheses and diagnostic definitions, of inflammatory fibrous hyperplasia, related to the use of removable prostheses. Conclusion: Thus, this study evidenced the importance of knowledge about the main alterations that affect the oral cavity of elderly patients, which allows better attention to this population, besides being a pioneering stomatological study in hospital units.

Sphenophorus levis Behavior Studies: Evaluating Insect Attractiveness or Repellency to One Insecticide Treatment and Assessing Nocturnal Insect Activity and Location Pattern.

Sphenophorus levis Vaurie, 1978 (Coleoptera: Curculionidae). is a difficult to control pest in sugarcane that causes great damage to the subterranean part of the plant. Low insect control is the result of the pesticide application technology adopted but also a consequence of the lack of studies regarding the pest's behavior. This research aimed to examine the attractiveness and repellency of one labelled insecticide dose to S. levis adults and to evaluate the activity and location behavior of S. levis adults under hourly observations over 24 h. Repellency and attractiveness studies were conducted in free-choice tests with treated soil with an insecticide product composed of lambda-cyhalothrin + thiamethoxam active ingredients and untreated soil. Insect activity and location behavior studies were assessed by conducting hourly observations of S. levis adults in containers with soil and sugarcane plant. The results indicate that S. levis adults are not repelled nor attracted to soil treated with the labelled dose of lambda-cyhalothrin + thiamethoxam in sugarcane. Additionally, insects presented nocturnal behavior for most activities (walking, digging and mating) starting at 6:00 p.m. until 2:00 a.m. An average of 21% of insects were out of the soil at night while the majority, 79%, remained inside the soil. During the day, most insects, 95%, remained hidden in the soil. Exposed insects were primarily located on the soil surface. According to these results, nocturnal insecticide applications may improve S. levis adult control due to greater insect activity and exposure at night.

Evaluation of Vitamin D isolated or Associated with Teriparatide in Peri-Implant Bone Repair in Tibia of Orchiectomized Rats.

(1) Background: The objective of this study was to evaluate the morphometry of peri-implant bone tissue in orchiectomized rats, treated with vitamin D isolated or associated with teriparatide. (2) Methods: 24 rats were divided into 4 groups: ORQ-orchiectomy, without drug treatment, ORQ+D-orchiectomy, treated with vitamin D, ORQTERI-orchiectomy, treated with teriparatide and ORQTERI+D-orchiectomy, treated with teriparatide + vitamin D. Each animal received an implant in the tibial metaphysis. Euthanasia occurred 60 days after implant surgery. Computed microtomography (micro-CT) was performed to evaluate the parameters of volume and percentage of bone volume (BV, BV/TV), trabecular thickness (Tb.Th), number and separation of trabeculae (Tb.N, Tb.Sp) and percentage of total porosity (Po-tot). Data were subjected to 1-way ANOVA and Tukey post-test, with a significance level of 5%. (3) Results: For the parameters BV, BV/TV, Tb.Th, the ORQTERI+D group showed the highest values in relation to the other groups and for Po-tot, the lowest values were for ORQTERI+D. For Tb.Sp and Tb.N, there was no statistically significant difference when comparing intragroup results (p > 0.05). (4) Conclusions: It was possible to conclude that treatment with vitamin D associated with teriparatide increases bone volume and improves bone quality.

Effect of Topical PTH 1-34 Functionalized to Biogran® in the Process of Alveolar Repair in Rats Submitted to Orchiectomy.

(1) Background: There are many therapies for osteoporosis control and bone maintenance; anabolic drugs such as teriparatide and bone grafts help in the repair process and stimulate bone formation. Thus, the aim of the present study was to evaluate the behavior of repaired bone in the presence of PTH (teriparatide) associated with Biogran® (biomaterial) through a sonochemical procedure after extraction in rats. (2) Methods: The insertion of Biogran® with PTH in the alveolus was performed 30 days after incisor extraction. Euthanasia occurred after 60 days. (3) Results: The use of local treatment of PTH loaded with Biogran® in healthy rats promoted good results for micro-CT, with an increase in percentage and bone volume, number and trabecular separation and less total porosity. Greater immunostaining for Wnt, ß-Catenin and osteocalcin proteins and lower expression for Thrombospondin-Related Adhesive Protein (TRAP), which shows an increase in the number of osteoblasts and inhibition of osteoclast action. However, the treated orchiectomized groups did not obtain such expressive results. (4) Conclusion: The use of Biogran® with PTH improved alveolar repair in rats. However, new researches with more efficient doses must be studied to collaborate effectively with the formation of a quality bone after the orchiectomy.

Different Stages of Alveolar Bone Repair Process Are Compromised in the Type 2 Diabetes Condition: An Experimental Study in Rats.

The aim of this study was to analyze the stages of the alveolar bone repair in type 2 diabetic rats evaluating the mechanism of mineralization and bone remodeling processes after dental extraction. Forty-eight rats were divided into normoglycemic (NG) and type 2 diabetes (T2D) groups. The upper right incisor was extracted and after 3, 7, 14 and 42 days the animals were euthanized. The following analyses were performed: immunolabeling against antibodies TNFα, TGFß, IL6, WNT, OCN and TRAP, collagen fibers maturation, microtomography and confocal microscopy. Data were submitted to statistical analysis. The immunolabeling analysis showed that the T2D presented a more pronounced alveolar inflammation than NG. Labeling of proteins responsible for bone formation and mineralization was higher in NG than T2D, which presented greater resorptive activity characterized by TRAP labeling. Also, T2D group showed a decrease in the amount of collagen fibers. Micro-CT analysis showed that T2D causes a decrease in bone volume percentage due to deficient trabecular parameters and higher porosity. The T2D bone dynamics show a loss in bone remodeling process. T2D prolongs the local inflammatory process, which impairs the organization and maturation of collagen fibers, delaying bone formation that generates impact on mineralization and bone turnover.

Physicochemical properties, droplet size and volatility of dicamba with herbicides and adjuvants on tank-mixture.

The adoption of dicamba-tolerant soybean varieties has increased the concern and demand for new drift and volatility reduction technologies. Potential spray nozzles and adjuvants should be studied to determine its effects on drift and volatility of dicamba tank-mixtures. The objective of this study was to evaluate physicochemical characteristics of spray solutions containing dicamba; to analyze droplet size effect with air induction nozzles; and to assess dicamba volatilization on soybean plants with a proposed methodology. Treatments included dicamba only and mixtures with herbicides and adjuvants. Dicamba mixed with lecithin + methyl soybean oil + ethoxylated alcohol adjuvant had the greatest efficacy potential among treatments considering tank-mixture pH, surface tension, contact angle and droplet size. The MUG11003 nozzle produced the coarsest droplet size and was better suited for drift management among nozzle types. The proposed volatilization methodology successfully indicated dicamba volatilization in exposed soybean plants and among the evaluated treatments, it showed greater volatilization for dicamba with glyphosate + lecithin + propionic acid adjuvant.

Impact of histological diagnosis on the treatment of atypical brainstem lesions.

For atypical brainstem lesions, histological diagnosis can have an impact on treatment, especially in cases where diffuse glioma is not found. Since radiotherapy is the only therapeutic modality that has shown clinical and radiographic improvement in patients with diffuse glioma, the misdiagnosis of diffuse glioma can have drastic consequences, particularly in patients with nontumorous lesions. Thus, the purpose of this study was to evaluate the impact of histological diagnosis on the treatment of atypical brainstem lesions. This was a retrospective study of 31 patients who underwent biopsy of atypical brainstem lesions. The procedures were performed between January 2008 and December 2018 at the Life Center Hospital and Santa Casa de Belo Horizonte, MG, Brazil. A diagnosis was obtained in 26 (83.9%) cases. Three patients presented complications: one presented bleeding with no clinical repercussions and two showed worsening of neurological deficit, only one of which was definitive. No mortality occurred due to the procedure. The histological diagnosis was diffuse glioma in seven cases (22.6%) and not diffuse glioma in 19 cases (61.3%). Thus, the histological diagnosis had an impact on the treatment of 19 patients (treatment impact rate: 61.3%). The histological diagnosis of intrinsic brainstem lesions is a safe, efficient procedure with a high diagnosis rate, and as such, it should be considered in the management of atypical lesions.

Psicopatologia na contemporaneidade: análise comparativa entre o DSM-IV e o DSM-V / Psychopathology in contemporary society: comparative analysis of the DSM-IV and DSM-V

O DSM (Diagnostic and Statistical Manual of Mental Disorders) é um sistema diagnóstico e estatístico de classificação dos transtornos mentais, segundo o modelo categorial, destinado à prática clínica e à pesquisa em psiquiatria elaborado pela APA (American Psychiatric Association). Esse manual é constantemente revisado e reeditado no intuito de atualizar suas classificações psicodiagnósticas. Nesse sentido, o presente artigo objetivou efetuar uma análise comparativa entre as classes e as categorias diagnósticas dos quadros clínicos referentes às perturbações de ansiedade, às alterações do humor e às perturbações relativas à infância e adolescência vigentes no DSM-IV e no DSM-V, de modo a mapear quais entidades clínicas foram incluídas, excluídas ou fundidas, formando novas classes e categorias diagnósticas na versão mais atualizada do manual. A metodologia utilizada foi a pesquisa bibliográfica e documental de caráter qualitativo, por meio de um estudo descritivo e de uma análise de dados de cunho comparativo. Os resultados referentes à comparação das classes e categorias diagnósticas apontam significativos acréscimos nos três eixos analisados que abrem as portas para se debater as consequências da patologização e medicalização de condições próprias do humano, como a TPM, a tristeza, os comportamentos enérgicos das crianças e os rebeldes dos adolescentes.(AU)

The Diagnostic and Statistical Manual of Mental Disorders (DSM) is a diagnostic and statistical system for classifying mental disorders according to the categorical model for clinical practice and research in psychiatry prepared by the APA (American Psychiatric Association). This manual is constantly revised and reissued to update its psychodiagnostic classifications. In this sense, the present article aimed to make a comparative analysis between the classes and the diagnostic categories of the clinical frameworks related to anxiety disorders, mood alterations and childhood and adolescent disorders in the DSM-IV and DSM-V, to map which clinical entities were included, excluded or merged, forming new diagnostic classes and categories in the most updated version of the manual. The methodology used was qualitative bibliographic and documentary research through a descriptive study and a comparative data analysis. The results regarding the comparison of diagnostic classes and categories point to significant increases in the three analyzed axes that open the doors to debate the consequences of the pathologization and medicalization of human conditions such as PMS, sadness, energetic behavior of children and rebels of teenagers.(AU)

Efeito do PTH tópico incrementado ao BioGran® no defeito peri-implantar em ratos submetidos à orquiectomia / Effect of topical PTH added to BioGran® on peri-implant defect in rats submitted to orchiectomy

Objetivo: Avaliar o PTH 1-34 em via tópico, funcionalizado ao BioGran® pela técnica da sonoquimica, nos defeitos peri-implantares em ratos submetidos à orquiectomia. Materiais e Métodos: 128 ratos foram divididos em 2 grupos: SHAM - cirurgia fictícia, ORQ - orquiectomia, os quais cada grupo destes foram subdivididos em 3 grupos CLOT (defeito peri-implantar, sem utilização de biomaterial); BG (defeito peri-implantar + utilização do BioGran®); BGPTH (defeito peri-implantar + BioGran® funcionalizado com PTH 1-34 tópica). Cada animal recebeu um implante na metáfise tibial. Aos 14 e 42 dias de pós-operatório, foram administrados os fluorocromos calceína e alizarina, respectivamente. A eutanásia ocorreu ao30º (análises ultraestrutural e EDX) e 60º dia (demais análises) após a instalação dos implantes. As peças foram analisadas pela imunoistoquímica (WNT, Beta Catenina, TRAP e OC), HE e RT-PCR (RUNX2, ALP e OC), além da análise biomecânica, microtomografia computadorizada (microCT) para avaliação dos parâmetros de percentual de volume ósseo (BV/TV), espessura do trabeculado (Tb.Th), número e separação de trabéculas (Tb.N, Tb.Sp) e percentual de porosidade total, (Po-tot) e Bone Implant Contact (BIC, pela i.S). Para microscopia confocal a laser, a mensuração da área dos fluorocromos, da taxa diária de aposição mineral (MAR), e área de osso neoformado (AON) e análise Elementar (EDX). A nível ultraestrutural foram realizadas as análise de MET. Os dados não paramétricos foram comparados pelo teste Kruskal-Wallis e paramétricos pelo teste ANOVA 1 ou 2 fatores. Como pós-teste, os testes de Holm-Sidak, ou Tukey foram aplicados, com nível de significância a 5%. Resultados: Para os parâmetros microtomográficos referente a volume e qualidade (BV/TV, Tb.Th, Tb.Sp e Tb.N) o bio material mostrou melhora em todos os grupos aplicados com ou sem PTH 1-34, já para BIC foi possível notar que o grupo ORQ-BGPH foi semelhante ao SHAM-BG (p>0,05). Para AON e analise dos fluorocromos a o biomaterial funcionalizado elevou a regeneração óssea local do animal orquiectomizado ao mesmo nível do animal saudável em que foi utilizado biomaterial com ou sem PTH 1-34. Conclusões: A funcionalização do BioGran® com PTH 1-34 apresenta melhora na regeneração óssea dos animais, sendo ainda mais eficaz nos animais submetidos a osteoporose(AU)

Objective: To evaluate PTH 1-34 in a topical way, functionalized to BioGran® by the sonochemistry technique, in peri-implant defects in rats submitted to orchiectomy. Materials and Methods: 128 rats were divided into 2 groups: SHAM - fictitious surgery, ORQ - orchiectomy, which each group was subdivided into 3 CLOT groups (periimplant defect, without biomaterial); BG (peri-implant defect + BioGran®); BGPTH (peri-implant defect + BioGran® functionalized with PTH 1-34). Each animal received an implant in the tibial metaphysis. At 14 and 42 days after surgery, calcein and alizarin fluorochromes were administered, respectively. Euthanasia occurred at the 30th (ultrastructural and EDX analysis) and 60th day (other analyzes) after the implant installation. The samples were analyzed by immunohistochemistry (WNT, Beta Catenin, TRAP and OC), HE and RT-PCR (RUNX2, ALP and OC), in addition to biomechanical analysis, microCT for evaluation of bone volume percentage parameters (BV/TV), trabecular thickness (Tb.Th), number and separation of trabeculae (Tb.N, Tb.Sp) and percentage of total porosity, (Po-tot) and Bone Implant Contact (BIC, by iS). For laser confocal microscopy, the measurement of the fluorochrome area, the mineral apposition rate (MAR), and the area of neoformed bone area (NBA) and elemental analysis (EDX). At the ultrastructural analysis, MET analyzes were performed. Nonparametric data were compared using the Kruskal-Wallis test and parametric data using the one and two- way ANOVA test. As a post-test, the HolmSidak or Tukey tests were applied, with a significance level of 5%. Results: For the microtomographic parameters referring to volume and quality (BV/TV, Tb.Th, Tb.Sp and Tb.N) the bio material showed improvement in all groups applied with or without PTH 1-34, for BIC it was possible to notice that the ORQ-BGPH group was similar to the SHAM-BG (p>0.05). For NBA and analysis of fluorochromes, the functionalized biomaterial increased the local bone regeneration of the orchiectomized animal to the same level as the healthy animal in which biomaterial was used with or without PTH 1- 34. Conclusions: The functionalization of BioGran® with PTH 1-34 shows improvement in bone regeneration in animals, being even more effective in animals submitted to osteoporosis(AU)

Care4Value: medição de valor em saúde em Unidades de Cuidados Continuados Integrados / Care4Value: medición del valor en salud en Unidades de Cuidados Continuos Integrados / Care4Value: measuring value in health in Integrated Continuous Care Units

Resumo Objetivo: Desenvolver uma plataforma digital para a otimização do processo de coleta de dados de escalas clínicas e monitoramento desses dados com vista à medição do valor em saúde. Métodos: Por meio de uma metodologia de investigação-ação, o desenvolvimento da plataforma incluiu abordagens qualitativas e quantitativas, em três fases: grupos focais com uma equipe multidisciplinar de investigadores e profissionais de saúde da UCCI do estudo-piloto; análise dos dados clínicos em formato de pré-teste de uma amostra de 21 usuários da UCCI para categorizar diferentes graus de complexidade; e, análise de informação financeira, aos custos operacionais da UCCI, relativa ao momento de permanência dos mesmos 21 usuários. O desenvolvimento iterativo e incremental da plataforma permitiu coletar feedback dos usuários como forma de melhoria. Resultados: A plataforma inclui 3 módulos: aplicativo móvel; dashboard; e módulo de importação. A plataforma centraliza os dados coletados e disponibiliza-os por meio de um dashboard . Os dados são coletados por aplicativo móvel e/ou por um módulo de importação que consome dados de sistemas clínicos existentes. Conclusão: O aplicativo móvel está apto a ser utilizado por profissionais de saúde e cuidadores, e o dashboard apresenta informações de acompanhamento clínico dos usuários e monitoramento dos seus ganhos em saúde.

Resumen Objetivo: Desarrollar una plataforma digital para optimizar el proceso de recolección de datos de escalas clínicas y monitoreo de estos datos con el fin de medir el valor en salud. Métodos: Mediante una metodología de investigación-acción, el desarrollo de la plataforma incluyó enfoques cualitativos y cuantitativos, en tres fases: grupos focales con un equipo multidisciplinario de investigadores y profesionales de la salud de la UCCI del estudio piloto, análisis de los datos clínicos en formato de prueba piloto de una muestra de 21 usuarios de la UCCI para categorizar los diferentes niveles de complejidad, y análisis de la información financiera, costos operativos de la UCCI, relativos al momento de permanencia de los mismos 21 usuarios. El desarrollo repetitivo y progresivo de la plataforma permitió recolectar feedback de los usuarios como forma de mejoría. Resultados: La plataforma incluye tres módulos: aplicación móvil, dashboard y módulo de importación. La plataforma centraliza los datos recolectados y los exhibe mediante un dashboard . Los datos son recolectados por aplicación móvil o por un módulo de importación que consume datos de sistemas clínicos existentes. Conclusión: La aplicación móvil es apta para ser utilizada por profesionales de la salud y cuidadores, y el dashboard presenta información de seguimiento clínico de los usuarios y monitoreo de sus ganancias en salud.

Abstract Objective: To develop a digital platform for optimizing data collection processes with medical scales and monitoring data for measuring value in health. Method: Using an investigative intervention methodology, a platform was developed including qualitative and quantitative approaches in three phases: focal groups were conducted by a multidisciplinary team of investigators and healthcare providers at the pilot study site, an Integrated Continuous Care Unit (UCCI); data from a sample of 21 UCCI users was analyzed as a pre-test to classify different levels of complexity; UCCI financial data and operational costs were collected and analyzed during the 21 users stay at the UCCI. The platform's iteractive and incremental development allowed for the collection of as a form of improvement. Results: The platform includes three modules: a mobile application; a dashboard; and an import module. Data collected on the platform are centralized and shown on the dashboard. Data are collected using a mobile application and/or an import module to input data from existing medical systems. Conclusion: The mobile application is ready to be used by healthcare providers and caregivers. The dashboard shows users' clinical follow-up and health gain data for monitoring.

Performance and feed efficiency of beef cattle fed high energy diet with probiotic consortium technology / Desempenho e eficiência alimentar de bovinos de corte alimentados com dieta de alto nível energético recebendo a tecnologia do consórcio probiótico

The objective of this study was to evaluate the effect of probiotic consortium on feed intake, feeding behavior, feed efficiency, fecal score, weight gain and carcass traits in finishing Nellore heifers fed high energy diets in feedlot. Twenty-four Nellore heifers were distributed in 12 pens with two animals each, being six pens per treatment. In the control treatment (CON) the animals were fed exclusively with the base diet, which contained 35% corn silage and 65% concentrate. In the treatment with probiotic consortium technology (TCP), the animals received the base diet and a single dose of 75 mL/animal/day of Bio Ciclo Completo (Global Saúde Brasil) top-dressed in the morning feeding. There was no period of adaptation to the diet. The experiment lasted 93 days. The use of TCP did not affect (P = 0.980) the dry matter intake. The average daily gain was 12.5% greater (P = 0.025) in the animals treated with TCP (0.941 kg/day for TCP versus 0.834 kg/day for CON). The animals that received TCP improved (P = 0.021) the feed efficiency by 12.6%. The animals that received TCP tended to have a better (P = 0.094) fecal score within the first 27 days of feedlot. There were no significant effects of TCP on the feeding behavior and carcass traits. Based on the results, there is a great potential of using TCP to improve productivity in beef cattle.(AU)

O objetivo desse trabalho foi avaliar o efeito do consórcio probiótico sobre consumo de alimento, comportamento alimentar, eficiência alimentar, escore fecal, ganho de peso e características de carcaça na terminação em confinamento de novilhas Nelore alimentadas com dieta de alta energia. Vinte e quatro novilhas da raça Nelore foram distribuídas em 12 baias com dois animais cada, sendo seis baias para cada tratamento. A dieta base continha 35% de silagem de milho e 65% de concentrado. No tratamento controle (CON) os animais foram alimentados exclusivamente com a dieta base. No tratamento com tecnologia de consórcio probiótico (TCP), os animais receberam, além da dieta base, uma dose única de 75 mL/animal/dia de Bio Ciclo Completo (Global Saúde Brasil) sobre a ração na alimentação matinal. Nenhum período de adaptação à dieta foi realizado. O período experimental durou 93 dias. O uso de TCP não alterou (P = 0,980) o consumo de matéria seca em relação ao CON. O ganho médio diário foi 12,5% maior (P = 0,025) nos animais tratados com TCP (0,941 kg/dia para TCP versus 0,834 kg/dia para CON). Os animais que receberam TCP melhoraram (P = 0,021) a eficiência alimentar em 12,6%. Os animais que receberam TCP tenderam a ter melhor (P = 0,094) escore fecal nos primeiros 27 dias de confinamento. Não houve efeitos significativos da TCP sobre o comportamento alimentar e características de carcaça. Com base nos resultados observados nesse estudo, existe um grande potencial de utilização do TCP para melhorar a produtividade em bovinos de corte.(AU)

Teriparatide improves alveolar bone modelling after tooth extraction in orchiectomized rats.

OBJECTIVE: The aim of this study was to evaluate bone metabolism in the alveolar repair process in orchiectomized male rats treated with teriparatide. MATERIALS AND METHODS: A total of 78 rats were divided into three groups: 26 oriectomized rats treated with teriparatide (ORQTRAT), 26 untreated orchiectomized rats (ORQ), and 26 rats which had undergone sham surgical procedures (SHAM), all these animals underwent extraction of the central incisor tooth. Thus, a histological analysis was performed (42 days). Real Time PCR (14 and 42 days) and immunohistochemical (42 days) analyses were performed based on the expression of RANK-L and osteoprotegerin (OPG). The calcein and alizarin were analyzed via laser confocal microscopy to verify alveolar bone turnover, and microtomographic analysis was performed to determine volume and bone quality (calcified tissues). In this analysis, the euthanasia period was 60 days post-extraction. The quantitative data were submitted to the statistical test and the significance level of 5% was adopted. RESULTS: Teriparatide increased bone turnover with a higher relative gene expression of RANKL and OPG at 14 days, and at 42 days, there was a significant decrease of RANKL and an increase of OPG, this standard can also be evaluated in the ratio of RANKL to OPG. Greater values of area and bone mineral deposition were found for the ORQTRAT group (p < 0.05), along with higher bone volume values (p < 0.05). The immunolabels revealed greater intensity in the relationship of RANKL and OPG, which led to intense remodeling aiding in the process of bone formation. CONCLUSION: The teriparatide treatment in orchiectomized rats increases bone volume and decreases the porosity, in addition to promoting greater intensity in bone turnover during alveolar repair.

Performance and feed efficiency of beef cattle fed high energy diet with probiotic consortium technology / Desempenho e eficiência alimentar de bovinos de corte alimentados com dieta de alto nível energético recebendo a tecnologia do consórcio probiótico

The objective of this study was to evaluate the effect of probiotic consortium on feed intake, feeding behavior, feed efficiency, fecal score, weight gain and carcass traits in finishing Nellore heifers fed high energy diets in feedlot. Twenty-four Nellore heifers were distributed in 12 pens with two animals each, being six pens per treatment. In the control treatment (CON) the animals were fed exclusively with the base diet, which contained 35% corn silage and 65% concentrate. In the treatment with probiotic consortium technology (TCP), the animals received the base diet and a single dose of 75 mL/animal/day of Bio Ciclo Completo (Global Saúde Brasil) top-dressed in the morning feeding. There was no period of adaptation to the diet. The experiment lasted 93 days. The use of TCP did not affect (P = 0.980) the dry matter intake. The average daily gain was 12.5% greater (P = 0.025) in the animals treated with TCP (0.941 kg/day for TCP versus 0.834 kg/day for CON). The animals that received TCP improved (P = 0.021) the feed efficiency by 12.6%. The animals that received TCP tended to have a better (P = 0.094) fecal score within the first 27 days of feedlot. There were no significant effects of TCP on the feeding behavior and carcass traits. Based on the results, there is a great potential of using TCP to improve productivity in beef cattle.

O objetivo desse trabalho foi avaliar o efeito do consórcio probiótico sobre consumo de alimento, comportamento alimentar, eficiência alimentar, escore fecal, ganho de peso e características de carcaça na terminação em confinamento de novilhas Nelore alimentadas com dieta de alta energia. Vinte e quatro novilhas da raça Nelore foram distribuídas em 12 baias com dois animais cada, sendo seis baias para cada tratamento. A dieta base continha 35% de silagem de milho e 65% de concentrado. No tratamento controle (CON) os animais foram alimentados exclusivamente com a dieta base. No tratamento com tecnologia de consórcio probiótico (TCP), os animais receberam, além da dieta base, uma dose única de 75 mL/animal/dia de Bio Ciclo Completo (Global Saúde Brasil) sobre a ração na alimentação matinal. Nenhum período de adaptação à dieta foi realizado. O período experimental durou 93 dias. O uso de TCP não alterou (P = 0,980) o consumo de matéria seca em relação ao CON. O ganho médio diário foi 12,5% maior (P = 0,025) nos animais tratados com TCP (0,941 kg/dia para TCP versus 0,834 kg/dia para CON). Os animais que receberam TCP melhoraram (P = 0,021) a eficiência alimentar em 12,6%. Os animais que receberam TCP tenderam a ter melhor (P = 0,094) escore fecal nos primeiros 27 dias de confinamento. Não houve efeitos significativos da TCP sobre o comportamento alimentar e características de carcaça. Com base nos resultados observados nesse estudo, existe um grande potencial de utilização do TCP para melhorar a produtividade em bovinos de corte.

Effect of intermittent teriparatide (PTH 1-34) on the alveolar healing process in orchiectomized rats.

OBJECTIVE: To evaluate intermittent teriparatide therapy's influence on the alveolar healing process in rats with induced osteoporosis. MATERIALS AND METHODS: Fifty-four male rats were divided into three groups: fictitious surgery (SHAM), bilateral orchiectomy (ORQ NT), and bilateral orchiectomy treated with teriparatide (ORQ TERI). Right upper incisor extraction was performed. After 14 and 42 days, the rats were euthanized. Immunolabeling analysis was performed in order to characterize bone turnover through Wnt, alcaline phosphatase, osteocalcin, and TRAP presence in tissue; micro-CT analysis was performed in order to determine the bone volume fraction (BV/TV), trabecular thickness, separation, and number (Tb.Th, Tb.Sp, Tb.N). For the micro-CT data, statistical analysis was performed through one-way ANOVA and Tukey post-test, for parametrical data, with significance level adopted in p < 0.05. Days, Wnt, alkaline phosphatase, and osteocalcin revealed more labeling for ORQ TERI and less for TRAP. For the Tb.N in the ORQ NT group was 0.496 mm, a significant statistical difference was observed between the groups of ORQ NT and ORQ TERI (p < 0.05). For the BV/TV, Tb.Sp, and Tb.Th parameters, no significant statistical difference was observed among the three experimental groups (p > 0.05). CONCLUSIONS: Treatment with intermittent teriparatide reverted impairment in the metabolism of repairing bone tissue in orchiectomized animals. CLINICAL RELEVANCE: Cases of decreased bone density such as osteoporosis can lead to delayed alveolar repair process. PTH 1-34 has been shown to be a medication that improves this repair, making bone of low quality into one with normal features.