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1.
Pain Med ; 22(2): 338-351, 2021 02 23.
Article in English | MEDLINE | ID: mdl-32875331

ABSTRACT

BACKGROUND: Chronic constriction injury (CCI) is a model of neuropathic pain induced by four loose ligatures around the sciatic nerve. This work aimed to investigate the sensory, affective, cognitive, and motor changes induced by an adaptation of the CCI model by applying a single ligature around the sciatic nerve. METHODS: Mechanical allodynia was measured from day 1 to day 28 postsurgery by the von Frey test. The beam walking test (BWT) was conducted weekly until 28 days after surgery. Anxiety- and depression-like behaviors, and cognitive performance were assessed through the open field (OF), forced swimming (FS), and novel object recognition (NOR) tests, respectively, 21 days after surgery. RESULTS: The two CCI models, both Bennett and Xie's model (four ligatures of the sciatic nerve) and a modification of it (one ligature), induced mechanical allodynia, increased immobility in the FS, and reduced recognition index in the NOR. The exploratory behavior and time spent in the central part of the arena decreased, while the defensive behavior increased in the OF. The animals subjected to the two CCI models showed motor alterations in the BWT; however, autotomy was observed only in the group with four ligatures and not in the group with a single ligature. CONCLUSIONS: Overall these results demonstrate that our adapted CCI model, using a single ligature around the sciatic nerve, induces sensory, affective, cognitive, and motor alterations comparable to the CCI model with four ligatures without generating autotomy. This adaptation to the CCI model may therefore represent an appropriate and more easily performed model for inducing neuropathic pain and study underlying mechanisms and effective treatments.


Subject(s)
Cognitive Dysfunction , Mononeuropathies , Neuralgia , Animals , Constriction , Disease Models, Animal , Hyperalgesia/epidemiology , Neuralgia/epidemiology , Neuralgia/etiology , Rats , Sciatic Nerve
2.
Neurochem Res ; 44(9): 2068-2080, 2019 Sep.
Article in English | MEDLINE | ID: mdl-31317507

ABSTRACT

The mechanisms underlying chronic and neuropathic pain pathology involve peripheral and central sensitisation. The medial prefrontal cortex (mPFC) seems to participate in pain chronification, and glutamatergic neurotransmission may be involved in this process. Thus, the aim of the present work was to investigate the participation of the prelimbic (PrL) area of the mPFC in neuropathic pain as well as the role of N-methyl D-aspartate (NMDA) glutamate receptors in neuropathic pain induced by a modified sciatic nerve chronic constriction injury (CCI) protocol in Wistar rats. Neural inputs to the PrL cortex were inactivated by intracortical treatment with the synapse blocker cobalt chloride (CoCl2, 1.0 mM/200 nL) 7, 14, 21, or 28 days after the CCI or sham procedure. The glutamatergic agonist NMDA (0.25, 1 or 4 nmol) or the selective NMDA receptor antagonist LY235959 (2, 4 or 8 nmol) was microinjected into the PrL cortex 21 days after surgery. CoCl2 administration in the PrL cortex decreased allodynia 21 and 28 days after CCI. NMDA at 1 and 4 nmol increased allodynia, whereas LY235959 decreased mechanical allodynia at the highest dose (8 nmol) microinjected into the PrL cortex. These findings suggest that NMDA receptors in the PrL cortex participate in enhancing the late phase of mechanical allodynia after NMDA-induced increases and LY235959-induced decreases in allodynia 21 days after CCI. The glutamatergic system potentiates chronic neuropathic pain by NMDA receptor activation in the PrL cortex. Mechanism of neuropathic pain. The infusion of CoCl2, a synapse activity blocker, into the prelimbic (PrL) division of the medial prefrontal cortex (mPFC) decreased the severity of mechanical allodynia, showing the late participation of the limbic cortex. The glutamatergic system potentiates chronic neuropathic pain via NMDA receptor activation in the PrL cortex.


Subject(s)
Neuralgia/metabolism , Peripheral Nerves/metabolism , Prefrontal Cortex/metabolism , Receptors, N-Methyl-D-Aspartate/metabolism , Animals , Cobalt/pharmacology , Hyperalgesia/drug therapy , Isoquinolines/pharmacology , Male , N-Methylaspartate/pharmacology , Neuralgia/drug therapy , Peripheral Nerve Injuries/drug therapy , Prefrontal Cortex/drug effects , Rats, Wistar , Synaptic Transmission/drug effects
3.
Shock ; 39(4): 389-96, 2013 Apr.
Article in English | MEDLINE | ID: mdl-23481491

ABSTRACT

A reduction of the neutrophil migration into the site of infection during cecal ligation and puncture-induced sepsis increases host mortality. Inhibition of heme oxygenase (HO) prevents this neutrophil paralysis and improves host survival in the cecal ligation and puncture model. Taking into account that almost 50% of all sepsis cases are a consequence of pneumonia, we designed the present study to determine the role of HO in an experimental model of pneumonia-induced sepsis. The objective of this study was to evaluate whether the inhibition of HO improves the outcome and pathophysiologic changes of sepsis induced by an intratracheal instillation of Klebsiella pneumoniae. The pretreatment of mice subjected to pneumonia-induced sepsis with ZnDPBG (zinc deuteroporphyrin 2,4-bis glycol), a nonspecific HO inhibitor, increased the number of neutrophils in the bronchoalveolar spaces, reduced the bacterial load at the site of infection, and prevented the upregulation of CD11b and the downregulation of CXCR2 on blood neutrophils. Moreover, the pretreatment with ZnDPBG decreased alveolar collapse, attenuating the deleterious changes in pulmonary mechanics and gas exchanges and, as a consequence, improved the survival rate of mice from 0% to ∼20%. These results show that heme oxygenase is involved in the pathophysiology of pneumonia-induced sepsis and suggest that HO inhibitors could be helpful for the management of this disease.


Subject(s)
Bacteremia/enzymology , Heme Oxygenase (Decyclizing)/antagonists & inhibitors , Immune System Diseases/enzymology , Klebsiella Infections/enzymology , Leukocyte Disorders/enzymology , Pneumonia, Bacterial/enzymology , Pulmonary Alveoli/enzymology , Acute Lung Injury/prevention & control , Animals , Bacteremia/microbiology , Bronchi/enzymology , Chemokines/metabolism , Creatine Kinase, MB Form/metabolism , Cytokines/metabolism , Deuteroporphyrins/pharmacology , Enzyme Inhibitors/pharmacology , Klebsiella Infections/microbiology , Klebsiella pneumoniae , Mice , Pneumonia, Bacterial/microbiology , Receptors, Interleukin-8B/metabolism
4.
Proc Natl Acad Sci U S A ; 110(9): 3603-8, 2013 Feb 26.
Article in English | MEDLINE | ID: mdl-23401543

ABSTRACT

It is well established that dorsal root ganglion (DRG) cells synthesize prostaglandin. However, the role that prostaglandin plays in the inflammatory hyperalgesia of peripheral tissue has not been established. Recently, we have successfully established a technique to inject drugs (3 µL) directly into the L5-DRG of rats, allowing in vivo identification of the role that DRG cell-derived COX-1 and COX-2 play in the development of inflammatory hyperalgesia of peripheral tissue. IL-1ß (0.5 pg) or carrageenan (100 ng) was administered in the L5-peripheral field of rat hindpaw and mechanical hyperalgesia was evaluated after 3 h. Administration of a nonselective COX inhibitor (indomethacin), selective COX-1 (valeryl salicylate), or selective COX-2 (SC-236) inhibitors into the L5-DRG prevented the hyperalgesia induced by IL-1ß. Similarly, oligodeoxynucleotide-antisense against COX-1 or COX-2, but not oligodeoxynucleotide-mismatch, decreased their respective expressions in the L5-DRG and prevented the hyperalgesia induced by IL-1ß in the hindpaw. Immunofluorescence analysis demonstrated that the amount of COX-1 and COX-2, constitutively expressed in TRPV-1(+) cells of the DRG, significantly increased after carrageenan or IL-1ß administration. In addition, indomethacin administered into the L5-DRG prevented the increase of PKCε expression in DRG membrane cells induced by carrageenan. Finally, the administration of EP1/EP2 (7.5 ng) or EP4 (10 µg) receptor antagonists into L5-DRG prevented the hyperalgesia induced by IL-1ß in the hindpaw. In conclusion, the results of this study suggest that the inflammatory hyperalgesia in peripheral tissue depends on activation of COX-1 and COX-2 in C-fibers, which contribute to the induction and maintenance of sensitization of primary sensory neurons.


Subject(s)
Cyclooxygenase 1/metabolism , Cyclooxygenase 2/metabolism , Ganglia, Spinal/enzymology , Hyperalgesia/enzymology , Hyperalgesia/pathology , Inflammation/enzymology , Inflammation/pathology , Membrane Proteins/metabolism , Animals , Carrageenan/pharmacology , Cyclooxygenase Inhibitors/administration & dosage , Cyclooxygenase Inhibitors/pharmacology , Enzyme Activation/drug effects , Ganglia, Spinal/drug effects , Ganglia, Spinal/pathology , Gene Knockdown Techniques , Hyperalgesia/complications , Indomethacin/administration & dosage , Indomethacin/pharmacology , Inflammation/complications , Interleukin-1beta/pharmacology , Lumbar Vertebrae/drug effects , Lumbar Vertebrae/pathology , Male , Protein Kinase C-epsilon/metabolism , Protein Transport/drug effects , Rats , Rats, Wistar , Receptors, Prostaglandin E/antagonists & inhibitors , Receptors, Prostaglandin E/metabolism , TRPV Cation Channels/metabolism
5.
Naunyn Schmiedebergs Arch Pharmacol ; 386(4): 311-8, 2013 Apr.
Article in English | MEDLINE | ID: mdl-23338711

ABSTRACT

Melanocortin is a potent anti-inflammatory molecule. However, little is known about the effect of melanocortin on acute inflammatory processes such as neutrophil migration. In the present study, we investigated the ability of [Nle4, D-Phe7]-melanocyte-stimulating hormone (NDP-MSH), a semisynthetic melanocortin compound, in the inhibition of neutrophil migration in carrageenin-induced peritonitis model. Herein, subcutaneous pretreatment with NDP-MSH decreased neutrophil trafficking in the peritoneal cavity in a dose-dependent manner. NDP-MSH inhibited vascular leakage, leukocyte rolling, and adhesion and reduced peritoneal macrophage inflammatory protein 2, but not TNF-alpha, IL-1beta, IL-10, and keratinocyte-derived chemokine production. In addition, the effect on neutrophil migration was reverted by the pretreatment with both propranolol (a nonselective beta-adrenergic antagonist) and mecamylamine (a nonselective nicotinic antagonist) but not by splenectomy surgery. Moreover, NDP-MSH intracerebroventricular administration inhibited neutrophil migration, indicating participation of the central nervous system. Our results propose that the NDP-MSH effect may be due to a spleen-independent neuro-immune pathway that efficiently regulates excessive neutrophil recruitment to tissues.


Subject(s)
Neutrophils/drug effects , Peritonitis/immunology , alpha-MSH/analogs & derivatives , Animals , Carrageenan , Cell Adhesion/drug effects , Cell Movement/drug effects , Chemokine CXCL2/immunology , Cytokines/immunology , Leukocyte Rolling/drug effects , Male , Mice , Neutrophils/physiology , Peritonitis/chemically induced , Receptors, Adrenergic/physiology , Receptors, Nicotinic/physiology , alpha-MSH/pharmacology
6.
Pharmacol Biochem Behav ; 103(3): 678-83, 2013 Jan.
Article in English | MEDLINE | ID: mdl-23178341

ABSTRACT

In recent years, evidence that sensitization of primary afferent nociceptors is an important event associated with chronic pain has been accumulating. The present study aimed to evaluate the participation of the prostaglandin and sympathetic components in the long-lasting sensitization of nociceptors induced by acute inflammation in mice. The intraplantar administration of carrageenan (100 µg) enhanced the nociceptive response to a small dose of PGE(2) (9 ng/paw) or dopamine (3 µg/paw) up to 30 days later. This long-lasting sensitization is dependent on dopaminergic and prostanoid systems, since the pre-treatment with chlorpromazine (3 µg/paw) or indomethacin (100 µg/paw), but not local (6 µg/paw) or systemic (6 mg/kg) treatment with morphine, prevented its development. In agreement with this idea, the previous intraplantar administration of hyperalgesic doses of PGE(2) or dopamine also induced long-lasting sensitization, which was fully prevented by pretreatment with EP(4) and D(1) antagonists, respectively. In summary, the present work described in mice a long-lasting sensitization of nociceptors, initiated by an acute inflammatory stimulation and dependent on dopaminergic and prostanoid systems. The present data represent new insights on the mechanisms of peripheral sensitization that could contribute to establish the basis of new therapeutic strategies for acute and chronic inflammatory pain.


Subject(s)
Central Nervous System Sensitization/physiology , Dopamine/physiology , Inflammation/physiopathology , Nociceptors/physiology , Prostaglandins/physiology , Animals , Anti-Inflammatory Agents, Non-Steroidal/pharmacology , Biphenyl Compounds/pharmacology , Carrageenan , Central Nervous System Sensitization/drug effects , Chlorpromazine/pharmacology , Dinoprostone/antagonists & inhibitors , Dinoprostone/pharmacology , Disease Models, Animal , Dopamine/pharmacology , Dopamine Antagonists/pharmacology , Hyperalgesia/drug therapy , Hyperalgesia/physiopathology , Indomethacin/pharmacology , Inflammation/chemically induced , Inflammation/drug therapy , Male , Mice , Morphine/pharmacology , Nociceptors/drug effects , Pain/drug therapy , Pain Measurement/drug effects , Prostaglandin Antagonists/pharmacology
7.
Biomed Res Int ; 2013: 253483, 2013.
Article in English | MEDLINE | ID: mdl-24490151

ABSTRACT

Acacia farnesiana lectin-like protein (AFAL) is a chitin-binding protein and has been classified as phytohaemagglutinin from Phaseolus vulgaris (PHA). Legume lectins are examples for structural studies, and this family of proteins shows a remarkable conservation in primary, secondary, and tertiary structures. Lectins have ability to reduce the effects of inflammation caused by phlogistic agents, such as carrageenan (CGN). This paper explains the anti-inflammatory activity of AFAL through structural comparison with anti-inflammatory legume lectins. The AFAL model was obtained by molecular modeling and molecular docking with glycan and carrageenan were performed to explain the AFAL structural behavior and biological activity. Pisum sativum lectin was the best template for molecular modeling. The AFAL structure model is folded as a ß sandwich. The model differs from template in loop regions, number of ß strands and carbohydrate-binding site. Carrageenan and glycan bind to different sites on AFAL. The ability of AFAL binding to carrageenan can be explained by absence of the sixth ß -strand (posterior ß sheets) and two ß strands in frontal region. AFAL can inhibit pathway inflammatory process by carrageenan injection by connecting to it and preventing its entry into the cell and triggers the reaction.


Subject(s)
Anti-Inflammatory Agents/chemistry , Inflammation/drug therapy , Models, Molecular , Plant Lectins/chemistry , Acacia , Animals , Anti-Inflammatory Agents/metabolism , Carrageenan/toxicity , Chitin/chemistry , Crystallography, X-Ray , Inflammation/chemically induced , Inflammation/pathology , Mice , Molecular Docking Simulation , Phytohemagglutinins/chemistry , Phytohemagglutinins/metabolism , Plant Lectins/administration & dosage , Plant Lectins/isolation & purification , Plant Lectins/metabolism , Protein Binding
8.
Acta Cir Bras ; 26 Suppl 2: 74-8, 2011.
Article in English | MEDLINE | ID: mdl-22030819

ABSTRACT

PURPOSE: To assess in vitro the correlation between the number of neurons and the sensitivity to cholinergic drugs and acetylcholinesterase activity in chagasic patients. METHODS: A 3 x 1 cm strip of the muscle layer of the anterior part of the stomach, always close to the angular incisure, was removed from 10 chronic chagasic patients (6 men) submitted to megaesophagus or megacolon surgery and from 10 non-chagasic patients (4 men) submitted to other types of surgery (control group), aged on average 52.3 and 50.1 years, respectively, for histological and pharmacological studies. The action of cholinergic drugs was investigated in isolated preparations according to the superfusion method of Ferreira and Costa, and acetylcholinesterase activity was determined by the method of Ellman. For neuron count, the strips were cut into 8 µm sections according to the method standardized by Alcântara. RESULTS: There was a difference in number of neurons between the chagasic (5,6) and control (7,3) groups. Acetylcholinesterase activity, in moles of hydrolyzed substrate per minute per gram tissue, was reduced in chagasic patients (4,32) compared to the controls (7,30). No hypersensitivity of the gastric musculature to cholinergic drugs was detected, with a reduced maximum response to carbachol and betanechol in the chagasic group. CONCLUSIONS: The reduction of neurons in the myenteric plexus of the stomach of chronic chagasic patients can be demonstrated even in the absence of clinical chagasic gastropathy. The hypersensitivity of the gastric musculature to cholinergic drugs probably depends on intense denervation. The reduced acetylcholinesterase activity demonstrates the involvement of the cholinergic innervation in the stomach of chronic chagasic patients. There was no correlation between number of neurons, sensitivity to cholinergic drugs and acetylcholinesterase activity in the gastric musculature of chagasic and non-chagasic patients.


Subject(s)
Acetylcholinesterase/metabolism , Chagas Disease/drug therapy , Cholinergic Agents/pharmacology , Muscle, Smooth/innervation , Myenteric Plexus/pathology , Stomach/innervation , Acetylcholine/pharmacology , Adult , Carbachol/pharmacology , Case-Control Studies , Cell Count , Chagas Disease/enzymology , Cholinergic Agonists/pharmacology , Esophageal Achalasia/pathology , Esophageal Achalasia/surgery , Female , Humans , Male , Middle Aged , Muscle, Smooth/drug effects , Muscle, Smooth/enzymology , Neurons/cytology , Stomach/drug effects , Stomach/enzymology
9.
Nitric Oxide ; 25(3): 243-54, 2011 Oct 30.
Article in English | MEDLINE | ID: mdl-21723953

ABSTRACT

Nitric oxide (NO) is involved in many physiological processes and several lines of evidence have indicated that NO plays a complex and diverse role in the modulation of pain. Nitric oxide is an important neurotransmitter involved in the nociceptive process and, in the dorsal horn of the spinal cord, it contributes to the development of central sensitization. On the other hand, experimental data have also demonstrated that NO inhibits nociception in the peripheral and also in the central nervous system. In addition, it has been shown that nitric oxide mediates the analgesic effect of opioids and other analgesic substances. The information included in the present review aims to present and analyze data about the dual effect of NO on pain transmission and control, the molecular mechanisms involved in these effects and also the potential use of nitric oxide in pain therapy.


Subject(s)
Analgesia/methods , Nitric Oxide/metabolism , Nociceptors/metabolism , Pain/metabolism , Animals , Humans , Nitric Oxide/therapeutic use , Pain/drug therapy , Pain/physiopathology , Pain Measurement
10.
Nitric oxide ; 25(3): 243-254, 2011.
Article in English | Sec. Est. Saúde SP, SESSP-IBPROD, Sec. Est. Saúde SP, SESSP-IBACERVO | ID: biblio-1064939

ABSTRACT

Nitric oxide (NO) is involved in many physiological processes and several lines of evidence have indicated that NO plays a complex and diverse role in the modulation of pain. Nitric oxide is an important neurotransmitter involved in the nociceptive process and, in the dorsal horn of the spinal cord, it contributes to the development of central sensitization. On the other hand, experimental data have also demonstrated that NO inhibits nociception in the peripheral and also in the central nervous system. In addition, it has been shown that nitric oxide mediates the analgesic effect of opioids and other analgesic substances. The information included in the present review aims to present and analyze data about the dual effect of NO on pain transmission and control, the molecular mechanisms involved in these effects and also the potential use of nitric oxide in pain therapy.


Subject(s)
Male , Female , Humans , Spinal Cord , Nitric Oxide/adverse effects , Central Nervous System
11.
Acta cir. bras ; 26(supl.2): 74-78, 2011. tab
Article in English | LILACS | ID: lil-602648

ABSTRACT

PURPOSE: To assess in vitro the correlation between the number of neurons and the sensitivity to cholinergic drugs and acetylcholinesterase activity in chagasic patients. METHODS: A 3x1 cm strip of the muscle layer of the anterior part of the stomach, always close to the angular incisure, was removed from 10 chronic chagasic patients (6 men) submitted to megaesophagus or megacolon surgery and from 10 non-chagasic patients (4 men) submitted to other types of surgery (control group), aged on average 52.3 and 50.1 years, respectively, for histological and pharmacological studies. The action of cholinergic drugs was investigated in isolated preparations according to the superfusion method of Ferreira and Costa, and acetylcholinesterase activity was determined by the method of Ellman. For neuron count, the strips were cut into 8 µm sections according to the method standardized by Alcântara. RESULTS: There was a difference in number of neurons between the chagasic (5,6) and control (7,3) groups. Acetylcholinesterase activity, in moles of hydrolyzed substrate per minute per gram tissue, was reduced in chagasic patients (4,32) compared to the controls (7,30). No hypersensitivity of the gastric musculature to cholinergic drugs was detected, with a reduced maximum response to carbachol and betanechol in the chagasic group. CONCLUSIONS: The reduction of neurons in the myenteric plexus of the stomach of chronic chagasic patients can be demonstrated even in the absence of clinical chagasic gastropathy. The hypersensitivity of the gastric musculature to cholinergic drugs probably depends on intense denervation. The reduced acetylcholinesterase activity demonstrates the involvement of the cholinergic innervation in the stomach of chronic chagasic patients. There was no correlation between number of neurons, sensitivity to cholinergic drugs and acetylcholinesterase activity in the gastric musculature of chagasic and non-chagasic patients.


OBJETIVO: Avaliar in vitro a correlação entre o número de neurônios e a sensibilidade a drogas colinérgicas e a atividade da acetilcolinesterase em pacientes chagásicos. MÉTODOS: Em 10 pacientes chagásicos crônicos (6 homens) submetidos à cirurgia de megaesôfago ou de megacólon e em 10 pacientes não chagásicos (4 homens) submetidos a outros tipos de cirurgia (grupo controle), respectivamente com idade média de 52,3 e 50,1 anos, retirou-se uma tira de 3x1 cm da camada muscular da parede anterior do estômago, sempre junto á cisura angular, que serviu para os estudos histológicos e farmacológicos. A ação de drogas colinérgicas foi feita em preparação isolada de acordo com o método de superfusão de Ferreira e Costa, e a determinação da atividade da acetilcolinesterase pelo método de Ellman. Para a contagem de neurônios a tira muscular foi submetida a cortes de 8 micra segundo método padronizado por Alcântara. RESULTADOS: Houve diferença do número de neurônios entre os grupos chagásico (5,6) e controle (7,3). A atividade da acetilcolinesterase mostrou-se diminuída nos chagásicos (4,32) expressa como número de moles do substrato hidrolisado por minuto por grama de tecido, em relação aos controles (7,30). Não se encontrou hipersensibilidade da musculatura gástrica a drogas colinérgicas, encontrando-se inclusive efeito máximo reduzido ao carbacol e betanecol no grupo chagásico. CONCLUSÕES: A redução de neurônios no plexo mioentérico do estômago de pacientes chagásicos crônicos pode ser demonstrada mesmo na ausência de gastropatia chagásica clínica. A hipersensibilidade da musculatura gástrica a drogas colinérgicas provavelmente depende de desnervação intensa. A redução da atividade da acetilcolinesterase demonstra o comprometimento da inervação colinérgica no estômago de pacientes chagásicos crônicos. Não houve correlação entre número de neurônios, sensibilidade a drogas colinérgicas e atividade da acetilcolinesterase na musculatura gástrica de pacientes chagásicos ou não chagásicos.


Subject(s)
Adult , Female , Humans , Male , Middle Aged , Acetylcholinesterase/metabolism , Chagas Disease/drug therapy , Cholinergic Agents/pharmacology , Muscle, Smooth/innervation , Myenteric Plexus/pathology , Stomach/innervation , Acetylcholine/pharmacology , Case-Control Studies , Cell Count , Carbachol/pharmacology , Chagas Disease/enzymology , Cholinergic Agonists/pharmacology , Esophageal Achalasia/pathology , Esophageal Achalasia/surgery , Muscle, Smooth/drug effects , Muscle, Smooth/enzymology , Neurons/cytology , Stomach/drug effects , Stomach/enzymology
12.
Biochem Pharmacol ; 77(5): 867-77, 2009 Mar 01.
Article in English | MEDLINE | ID: mdl-19073148

ABSTRACT

In the present study, the participation of the Na(V)1.8 sodium channel was investigated in the development of the peripheral pro-nociceptive state induced by daily intraplantar injections of PGE(2) in rats and its regulation in vivo by protein kinase A (PKA) and protein kinase C epsilon (PKCvarepsilon) as well. In the prostaglandin E(2) (PGE(2))-induced persistent hypernociception, the Na(V)1.8 mRNA in the dorsal root ganglia (DRG) was up-regulated. The local treatment with dipyrone abolished this persistent hypernociception but did not alter the Na(V)1.8 mRNA level in the DRG. Daily intrathecal administrations of antisense Na(V)1.8 decreased the Na(V)1.8 mRNA in the DRG and reduced ongoing persistent hypernociception. Once the persistent hypernociception had been abolished by dipyrone, but not by Na(V)1.8 antisense treatment, a small dose of PGE(2) restored the hypernociceptive plateau. These data show that, after a period of recurring inflammatory stimuli, an intense and prolonged nociceptive response is elicited by a minimum inflammatory stimulus and that this pro-nociceptive state depends on Na(V)1.8 mRNA up-regulation in the DRG. In addition, during the persistent hypernociceptive state, the PKA and PKCvarepsilon expression and activity in the DRG are up-regulated and the administration of the PKA and PKCvarepsilon inhibitors reduce the hypernociception as well as the Na(V)1.8 mRNA level. In the present study, we demonstrated that the functional regulation of the Na(V)1.8 mRNA by PKA and PKCvarepsilon in the primary sensory neuron is important for the development of the peripheral pro-nociceptive state induced by repetitive inflammatory stimuli and for the maintenance of the behavioral persistent hypernociception.


Subject(s)
Cyclic AMP-Dependent Protein Kinases/metabolism , Inflammation/physiopathology , Nerve Tissue Proteins/physiology , Pain/physiopathology , Protein Kinase C-epsilon/metabolism , Sodium Channels/physiology , Animals , Base Sequence , DNA Primers , Dinoprostone/administration & dosage , Enzyme Activation , Inflammation/chemically induced , Male , NAV1.8 Voltage-Gated Sodium Channel , Pain/enzymology , Polymerase Chain Reaction , Rats , Rats, Wistar
13.
Proc Natl Acad Sci U S A ; 103(25): 9721-5, 2006 Jun 20.
Article in English | MEDLINE | ID: mdl-16766656

ABSTRACT

IL-15 is closely associated with inflammatory diseases. IL-15 targeting is effective in treating experimental and clinical rheumatoid arthritis (RA). Because hyperalgesia accompanies RA, we investigated the ability of IL-15 to induced nociceptor sensitization (hypernociception). We report here that IL-15 induced time- and dose-dependent mechanical hypernociception in mice. IL-15-induced hypernociception was inhibited by treatment with a dual endothelin receptor type A (ET(A))/endothelin receptor type B (ET(B)) antagonist (bosentan), ET(A) receptor antagonist (BQ123), or cyclooxygenase inhibitor (indomethacin). Moreover, IL-15 failed to induce hypernociception in IFN-gamma(-/-) mice, suggesting that IL-15 mediated hypernociception via an IFN-gamma-, endothelin (ET)-, and prostaglandin-dependent pathway. Consistent with this finding, IFN-gamma and ET-1 induced dose- and time-dependent mechanical hypernociception that was inhibited by BQ123 or indomethacin but not BQ788 (an ET(B) receptor antagonist). IFN-gamma induced the production of ET-1 and the expression of its mRNA precursor (preproET-1, PPET-1). Moreover, IL-15 also induced ET-1 production and PPET-1 mRNA expression in an IFN-gamma-dependent manner. Prostaglandin E(2) (PGE(2)) production was induced by IL-15, IFN-gamma, or ET-1. We also found that hypernociception induced by ovalbumin (OVA) in OVA-immunized mice was significantly diminished by treatment with sIL-15Ralpha (soluble IL-15 receptor alpha-chain), bosentan, BQ123, or indomethacin. Furthermore, OVA challenge induced the expression of PPET-1 mRNA in WT mice but not in IFN-gamma(-/-) mice. The PPET-1 mRNA expression was also inhibited by sIL-15Ralpha pretreatment. Therefore, our results demonstrate the sequential mechanical hypernociceptive effect of IL-15 --> IFN-gamma --> ET-1 --> PGE(2) and suggest that these molecules may be targets of therapeutic intervention in antigen-induced hypernociception.


Subject(s)
Endothelins/metabolism , Interferon-gamma/metabolism , Interleukin-15/pharmacology , Prostaglandins/metabolism , Animals , Endothelins/pharmacology , Gene Expression Regulation , Inflammation/chemically induced , Inflammation/immunology , Inflammation/metabolism , Interferon-gamma/deficiency , Interferon-gamma/genetics , Interferon-gamma/pharmacology , Mice , Ovalbumin/pharmacology , Prostaglandins/genetics , RNA, Messenger/genetics
14.
Neurosci Lett ; 386(2): 72-7, 2005 Sep 30.
Article in English | MEDLINE | ID: mdl-16043287

ABSTRACT

We previously described an animal model of persistent inflammatory sensitization of nociceptors. In this model the hypernociception persists for more than 30 days after the cessation of 2 weeks of daily intraplantar treatment with prostaglandin E(2) (PGE(2)). The tetrodotoxin-resistant (TTX-R) voltage-gated sodium channel Na(V)1.8 is considered a characteristic of primary afferent nociceptive C fibers and plays an important role in acute hypernociception. In the present study, the relevance of the Na(V)1.8 channel was investigated in this model of persistent mechanical hypernociception in rats. In the PGE(2)-induced persistent hypernociception, but not in the single injection-induced acute hypernociception, the mRNA expression (RT-PCR) of Na(V)1.8 in dorsal root ganglia (DRG) was up-regulated. A similar increase of Na(V)1.8 mRNA was observed when DbcAMP was used to induce persistent hypernociception. Four daily intrathecal administrations of oligodeoxynucleotides (ODN) antisense against Na(V)1.8 decreased the mRNA encoding Na(V)1.8 in DRG. The intrathecal administration of ODN antisense prevented the PGE(2)-induced acute hypernociception and significantly reduced ongoing PGE(2)-induced persistent hypernociception. A parallel restoration of the persistent hypernociception and up-regulation of Na(V)1.8 mRNA was observed after the cessation of ODN antisense treatment. These results suggest the participation of Na(V)1.8 channels in the development and maintenance of chronic inflammatory hyperalgesia, and confirm their involvement in the acute inflammatory hypernociception.


Subject(s)
Inflammation/physiopathology , Nerve Tissue Proteins/biosynthesis , Pain/physiopathology , Sodium Channels/biosynthesis , Animals , Chronic Disease , Dinoprostone/toxicity , Disease Models, Animal , Ganglia, Spinal/drug effects , Ganglia, Spinal/metabolism , Inflammation/chemically induced , Male , NAV1.8 Voltage-Gated Sodium Channel , Nerve Tissue Proteins/drug effects , Nociceptors/metabolism , Oligonucleotides, Antisense/pharmacology , Pain Measurement , RNA, Messenger/analysis , Rats , Reverse Transcriptase Polymerase Chain Reaction , Sodium Channel Blockers/pharmacology , Sodium Channels/drug effects , Up-Regulation
15.
Br J Pharmacol ; 139(6): 1135-45, 2003 Jul.
Article in English | MEDLINE | ID: mdl-12871832

ABSTRACT

1. We investigated the mediators responsible for mechanical hypersensitivity induced by antigen challenge in rats immunised with ovalbumin (OVA). 2. Challenge with OVA (12.5-100 micro g, intraplantar) caused a dose- and time-dependent mechanical hypersensitivity, which peaked 3 h after, decreased thereafter and reached control levels 24 h later. 3. Levels of TNFalpha, IL-1beta and cytokine-induced neutrophil chemoattractant 1 (CINC-1) were increased in paw skin after antigen challenge. 4. OVA-evoked hypersensitivity was partially inhibited (about 51%) by pretreatment with anti-TNFalpha, IL-1beta and IL-8 sera or with IL-1 receptor antagonist (IL-1ra), but not anti-NGF serum. Pretreatment with thalidomide (45 mg kg(-1)) or pentoxifylline (100 mg kg(-1)) also partially inhibited the hypersensitivity at 1-3 h after challenge. 5. Pretreatment with indomethacin (5 mg kg(-1)) or atenolol (1 mg kg(-1)) reduced the OVA-induced hypersensitivity at 1 and 3 h, but not at 5 h after challenge, while the combination of B(1) and B(2) bradykinin receptor antagonists was ineffective over the same times. 6. Pretreatment with MK886 (5-lipoxygenase-activating protein inhibitor, 3 mg kg(-1)), CP 105696 (LTB(4) receptor antagonist; 3 mg kg(-1)) or dexamethasone (0.5 mg kg(-1)) inhibited the hypersensitivity from 1 to 5 h. Furthermore, LTB(4) levels were increased in the paw skin of challenged rats. 7. In conclusion, our results suggest that the TNFalpha-, IL-1beta- and CINC-1-driven release of prostaglandins, sympathetic amines and LTB(4) mediates the first 3 h of mechanical hypersensitivity induced by antigen challenge in rats. At 5 h after OVA administration, although TNFalpha has some role, LTB(4) is the critical nociceptive mediator.


Subject(s)
Antigens/administration & dosage , Antigens/immunology , Hyperalgesia/immunology , Hyperalgesia/metabolism , Leukotriene B4/physiology , Animals , Dose-Response Relationship, Drug , Dose-Response Relationship, Immunologic , Hypersensitivity/immunology , Hypersensitivity/metabolism , Injections, Subcutaneous , Leukotriene B4/biosynthesis , Male , Ovalbumin/administration & dosage , Ovalbumin/immunology , Pain Measurement/methods , Physical Stimulation/methods , Rats , Rats, Wistar , Skin/immunology , Skin/metabolism , Up-Regulation/immunology
16.
Proc Natl Acad Sci U S A ; 100(5): 2923-8, 2003 Mar 04.
Article in English | MEDLINE | ID: mdl-12589028

ABSTRACT

The present study investigated whether activation of presynaptic N-methyl-d-aspartate (NMDA) receptors in the spinal cord produces a retrograde nociceptor sensitization (hypernociception) to mechanical nonnoxious stimulus. By using an electronic version of the von Frey hair test (pressure meter), s.c. intraplantar administration of prostaglandin E(2) (PGE(2)) (50-400 ng per paw) evoked a dose-related ipsilateral paw hypernociception. In contrast, intrathecal (i.t.) administration of NMDA (5-80 ng) and PGE(2) (15-150 ng) evoked dose-related bilateral paw hypernociception. The s.c. intraplantar administration of dipyrone (80-320 microg per paw) or morphine (3 and 9 microg per paw), usually used to antagonize peripheral PGE(2) (100 ng per paw), induced hypernociception and also antagonized the ipsilateral (without affecting the contralateral) paw hypernociception induced by i.t. injections of NMDA (40 ng) or PGE(2) (50 ng). These doses of drugs did not modify the basal mechanical sensitivity of control paws. This result shows that intraspinal NMDA or PGE(2) produces sensitization of the primary sensory neuron in response to mechanical stimulation. In a second series of experiments it was shown that the i.t. treatment with NaV1.8 (SNS/PN3) sodium channel antisense oligodeoxynucleotides, but not mismatch oligodeoxynucleotides, decreased the mRNA expression of sodium tetrodotoxin-resistant channels on the dorsal root ganglia and abolished the mechanical hypernociception induced by i.t. administration of NMDA. Thus, our results support the suggestion that glutamate release in the spinal cord during inflammation causes retrograde hypernociception of nociceptors associated with sodium tetrodotoxin-resistant channels in primary nociceptive sensory neurons.


Subject(s)
Neuropeptides/genetics , Neuropeptides/physiology , Nociceptors/physiology , Receptors, N-Methyl-D-Aspartate/metabolism , Sodium Channels/genetics , Sodium Channels/physiology , Analgesics, Opioid/pharmacology , Animals , Anti-Inflammatory Agents, Non-Steroidal/pharmacology , Dipyrone/pharmacology , Dose-Response Relationship, Drug , Ganglia, Spinal/metabolism , Glutamates/metabolism , Inflammation , Morphine/pharmacology , NAV1.8 Voltage-Gated Sodium Channel , Nociceptors/drug effects , Oligonucleotides, Antisense/pharmacology , Pain Measurement , Polymerase Chain Reaction , RNA, Messenger/metabolism , Rats , Rats, Wistar , Reverse Transcriptase Polymerase Chain Reaction , Spinal Cord/metabolism , Time Factors
17.
Shock ; 19(1): 61-5, 2003 Jan.
Article in English | MEDLINE | ID: mdl-12558146

ABSTRACT

Neutrophil migration to an infectious focus is essential for control and resolution of infection. Early studies demonstrated that the failure of such migration is observed in lethal sepsis induced by cecal ligation and puncture (L-CLP), whereas intense neutrophil migration is seen in sublethal CLP (SL-CLP). In this study, we found that inhibition of synthesis of prostaglandins or leukotriene B4 (LTB4) did not modify the failure of neutrophil migration or the survival rate of L-CLP mice. In addition, pretreatment of L-CLP mice with a platelet activating factor (PAF) receptor antagonist (UK74505), despite not interfering with the failure process, significantly increased (33%) the survival rate of the animals. Inhibitors of prostaglandin synthesis (indomethacin and meloxican) and UK74505 did not modify the neutrophil migration observed in SL-CLP. On the other hand, the blockade of LTB4 synthesis (MK886, a 5-lipoxygenase-activating protein inhibitor) or of its receptors (CP-105,696) resulted in reduced neutrophil migration to the peritoneal cavity in SL-CLP mice (62% and 60%, respectively), a consequent increase in the number of bacteria in the inflammatory focus, and a reduced survival rate of the animals (43% and 38%, respectively). Both SL-CLP and L-CLP animals presented significant levels of LTB4 in the peritoneal exudate (3- and 8-fold higher than sham group, respectively) and these were reduced by the pretreatment of mice with LTB4 inhibitors. In conclusion, our results suggest that LTB4, but not prostaglandins or PAF, is an important chemoattractant involved in neutrophil recruitment to infection sites in SL-CLP, a crucial event in confining the invading pathogens to a restricted area. However, in circumstances in which the infection turns to a lethal sepsis, LTB4 is not involved in the observed failure of neutrophil migration to the infectious focus.


Subject(s)
Cecum/surgery , Cell Movement , Leukotriene B4/blood , Leukotriene B4/physiology , Neutrophils/cytology , Wounds, Penetrating , Animals , Dihydropyridines/pharmacology , Eicosanoids/metabolism , Imidazoles/pharmacology , Indoles/pharmacology , Inflammation , Leukotrienes/metabolism , Lipoxygenase Inhibitors/pharmacology , Male , Mice , Mice, Inbred C57BL , Neutrophils/metabolism , Peritoneum/metabolism , Platelet Activating Factor/metabolism , Prostaglandins/metabolism , Time Factors
20.
Nitric Oxide ; 9(3): 153-64, 2003 Nov.
Article in English | MEDLINE | ID: mdl-14732339

ABSTRACT

There is controversy in the literature over whether nitric oxide (NO) released during the inflammatory process has a pro- or inhibitory effect on neutrophil migration. The aim of the present investigation was to clarify this situation. Treatment of rats with non-selective, NG-nitro-L-arginine (nitro), or selective inducible NO synthase (iNOS), aminoguanidine (amino) inhibitors enhanced neutrophil migration 6h after the administration of low, but not high, doses of carrageenan (Cg) or Escherichia coli endotoxin (LPS). The neutrophil migration induced by N-formyl-methionyl-leucyl-phenylalanine (fMLP) was also enhanced by nitro or amino treatments. The enhancement of Cg-induced neutrophil migration by NOS inhibitor treatments was reversed by co-treatment with L-arginine, suggesting an involvement of the L-arginine/NOS pathway in the process. The administration of Cg in iNOS deficient (iNOS(-/-)) mice also enhanced the neutrophil migration compared with wild type mice. This enhancement was markedly potentiated by treatment of iNOS(-/-) mice with nitro. Investigating the mechanisms by which NOS inhibitors enhanced the neutrophil migration, it was observed that they promoted an increase in Cg-induced rolling and adhesion of leukocytes to endothelium and blocked the apoptosis of emigrated neutrophils. Similar results were observed in iNOS(-/-) mice, in which these mechanisms were potentiated and reverted by nitro and L-arginine treatments, respectively. In conclusion, these results suggest that during inflammation, NO released by either constitutive NOS (cNOS) or iNOS down-modulates the neutrophil migration. This NO effect seems to be a consequence of decreased rolling and adhesion of the neutrophils on endothelium and also the induction of apoptosis in migrated neutrophils.


Subject(s)
Apoptosis , Chemotaxis, Leukocyte , Inflammation/immunology , Neutrophils/immunology , Nitric Oxide/physiology , Animals , Carrageenan/pharmacology , Cell Adhesion , Enzyme Inhibitors/pharmacology , Guanidines/pharmacology , Inflammation/pathology , Leukocyte Rolling , Lipopolysaccharides/pharmacology , Male , Mice , Mice, Knockout , N-Formylmethionine Leucyl-Phenylalanine/pharmacology , Neutrophils/pathology , Nitric Oxide Synthase/antagonists & inhibitors , Nitric Oxide Synthase/genetics , Nitric Oxide Synthase Type II , Nitroarginine/pharmacology , Rats , Rats, Wistar
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