ABSTRACT
Two mutations - Factor V Leiden (1691G > A) and the 20210G > A on the Prothrombin gene - are key risk factors for a frequent and potentially fatal disorder called Venous Thromboembolism. These molecular alterations can be investigated using real-time Polymerase Chain Reaction (PCR) with Fluorescence Resonance Energy Transfer (FRET) probes and distinct DNA pools for both factors. The objective of this paper is to present an application of Taguchi Experimental Design Method to determine the best parameters adjustment of a Molecular Assays Process in order to obtain the best diagnostic result for Venous Thromboembolism investigation. The complete process contains six three-level factors which usually demands 729 experiments to obtain the final result, if using a Full Factorial Array. In this research, a Taguchi L27 Orthogonal Array is chosen to optimize the analysis and reduce the number of experiments to 27 without degrading the final result accuracy. The application of this method can lessen the time and cost necessary to achieve the best operation condition for a required performance. The results is proven in practice and confirmed that the Taguchi method can really offer a good approach for clinical assay efficiency and effectiveness improvement even though the clinical diagnostics can be based on the use of qualitative techniques.
Subject(s)
DNA Mutational Analysis/methods , Factor V/genetics , Fluorescence Resonance Energy Transfer/methods , Prothrombin/genetics , Real-Time Polymerase Chain Reaction/methods , Venous Thromboembolism , Algorithms , Factor Analysis, Statistical , Genotype , Humans , Mutation , Venous Thromboembolism/diagnosis , Venous Thromboembolism/geneticsABSTRACT
Testou-se o efeito, sobre a formação da placa bacteriana, do sulfato de cobre a 0,35 por cento, do cloreto de cetílpiridineo (Cepacol-flúor) e do fluoreto estanhoso a 0,4 por cento, como únicos meios de higienização, durante 4 dias. Concluiu-se que não houve diferença estatisticamente significante entre dois índices de placa bacteriana do grupo controle e o que usou o sulfato de cobre como bochecho; que existe diferença significativa, a nível de 10 por cento, entre o grupo controle e o grupo que bochechou com Cepacol-flúor e a nível de 5 por cento entre o grupo controle e o grupo que usou o fluoreto estanhoso
Subject(s)
Humans , Male , Female , Adult , Mouthwashes , Dental Plaque/prevention & controlABSTRACT
Na presente pesquisa, testou-se o efeito, sobre a formação da placa bacteriana, da escovação orientada, do fluoreto estanhoso a 0,4//e da clorexidina a 0,2//, durante 4 dias, através do índice de Podshadley e Halley. Conclue-se que todos três fatores reduzem significativamente a formação da placa bacteriana
