ABSTRACT
Basal levels of oxidants are indispensible for redox signaling to produce adaptive cellular responses such as vitagenes linked to cell survival; however, at higher levels, they are detrimental to cells, contributing to aging and to the pathogenesis of numerous age-related diseases. Aging is a complex systemic process and the major gap in aging research reminds the insufficient knowledge about pathways shifting from normal "healthy" aging to disease-associated pathological aging. The major complication of normal "healthy" aging is in fact the increasing risk of age-related diseases such as cardiovascular diseases, diabetes mellitus, and neurodegenerative pathologies that can adversely affect the quality of life in general, with enhanced incidences of comorbidities and mortality. In this context, global "omics" approaches may help to dissect and fully study the cellular and molecular mechanisms of aging and age-associated processes. The proteome, being more close to the phenotype than the transcriptome and more stable than the metabolome, represents the most promising "omics" field in aging research. In the present study, we exploit recent advances in the redox biology of aging and discuss the potential of proteomics approaches as innovative tools for monitoring at the proteome level the extent of protein oxidative insult and related modifications with the identification of targeted proteins.
Subject(s)
Aging/metabolism , Oxidative Stress , Proteins/analysis , Proteomics , Age Factors , Animals , Biomarkers/analysis , Disease Susceptibility , Glutathione/analysis , Glutathione/metabolism , Humans , Lipid Peroxidation , Oxidation-Reduction , Phenotype , Predictive Value of Tests , Protein Carbonylation , Proteins/chemistry , Proteins/metabolism , Proteomics/methods , Risk Factors , Tyrosine/analogs & derivatives , Tyrosine/metabolismABSTRACT
Three aphasic patients are described whose speech contains invented word-forms which are legal combinations of meaningful parts of real words, like "fratellismo" (brother + ness) instead of "fratellanza" (brother + hood), and from combinations of meaningless and meaningful parts, like "terness + ico" (where "ico" is a real adjectival ending). These phenomena are previously unreported. On the assumption that brain-damaged patients use residual rather than novel abilities, these forms, along with the patients' predominant use of real compound words, indicate that speakers have a procedure for composing polymorphemic words online, but this is employed just when trying to find a whole word fails.
