Diversification of photoperiodic response patterns in a collection of early-flowering mutants of Arabidopsis.

Many plant species exhibit seasonal variation of flowering time in response to daylength. Arabidopsis (Arabidopsis thaliana) flowers earlier under long days (LDs) than under short days (SDs). This quantitative response to photoperiod is characterized by two parameters, the critical photoperiod (Pc), below which there is a delay in flowering, and the ceiling photoperiod (Pce), below which there is no further delay. Thus Pc and Pce define the thresholds beyond which maximum LD and SD responses are observed, respectively. We studied the quantitative response to photoperiod in 49 mutants selected for early flowering in SDs. Nine of these mutants exhibited normal Pce and Pc, showing that their precocious phenotype was not linked to abnormal measurement of daylength. However, we observed broad diversification in the patterns of quantitative responses in the other mutants. To identify factors involved in abnormal measurement of daylength, we analyzed the association of these various patterns with morphogenetic and rhythmic defects. A high proportion of mutants with altered Pce exhibited abnormal hypocotyl elongation in the dark and altered circadian periods of leaf movements. This suggested that the circadian clock and negative regulators of photomorphogenesis may contribute to the specification of SD responses. In contrast, altered Pc correlated with abnormal hypocotyl elongation in the light and reduced photosynthetic light-input requirements for bolting. This indicated that LD responses may be specified by positive elements of light signal transduction pathways and by regulators of resource allocation. Furthermore, the frequency of circadian defects in mutants with normal photoperiodic responses suggested that the circadian clock may regulate the number of leaves independently of its effect on daylength perception.

Comparison of environmental and mutational variation in flowering time in Arabidopsis.

Developmental dynamics can be influenced by external and endogenous factors in a more or less analogous manner. To compare the phenotypic effects of (i) environmental [i.e. standard (stPhP) and extended (exPhP) photoperiods] changes in Arabidopsis wild types and (ii) endogenous genetic variation in eav1-eav61 early flowering mutants, two temporal indicators were analysed, the time to bolting (DtB) and the number of leaves (TLN). It was found that DtB and TLN are differentially affected in different environmental and genetic contexts, and some factors of dynamic convergence were identified. The quantitative response to photoperiod is markedly contingent on the phototrophic input for DtB, but less so for TLN. To discriminate the light quantity and period components in DtB, two novel temporal indicators were determined, LtB (photosynthetic time to bolting) and PChron (DtB h(-1) of photoperiod), respectively. The use of PChron results in a coincidence of the variation profiles across stPhP and exPhP, interpreted as a buffering of the trophic response. Unlike natural accessions and later flowering mutants, the variation profiles across stPhP and eav mutants are significantly divergent, pointing to differences in environmental and genetic variation in flowering time. Yet, phenocopy effects and dynamic convergence between wild-type and mutant profiles are detected by using exPhP and the LtB indicator. Additional analyses of the cauline leaf number (CLN) show that the apical and basal boundaries of the primary inflorescence vary co-ordinately. The finding that the correlativity between CLN and TLN changes across photoperiods suggests that different states of intra-connectedness are involved in ontogenetic specification of flowering time and embodied in the primary inflorescence.

Extensive phenotypic variation in early flowering mutants of Arabidopsis.

Flowering time, the major regulatory transition of plant sequential development, is modulated by multiple endogenous and environmental factors. By phenotypic profiling of 80 early flowering mutants of Arabidopsis, we examine how mutational reduction of floral repression is associated with changes in phenotypic plasticity and stability. Flowering time measurements in mutants reveal deviations from the linear relationship between the number of leaves and number of days to bolting described for natural accessions and late flowering mutants. The deviations correspond to relative early bolting and relative late bolting phenotypes. Only a minority of mutants presents no detectable phenotypic variation. Mutants are characterized by a broad release of morphological pleiotropy under short days, with leaf characters being most variable. They also exhibit changes in phenotypic plasticity across environments for florigenic-related responses, including the reaction to light and dark, photoperiodic behavior, and Suc sensitivity. Morphological pleiotropy and plasticity modifications are differentially distributed among mutants, resulting in a large diversity of multiple phenotypic changes. The pleiotropic effects observed may indicate that floral repression defects are linked to global developmental perturbations. This first, to our knowledge, extensive characterization of phenotypic variation in early flowering mutants correlates with the reports that most factors recruited in floral repression at the molecular genetic level correspond to ubiquitous regulators. We discuss the importance of functional ubiquity for floral repression with respect to robustness and flexibility of network biological systems.

Down-regulation of the AtCCR1 gene in Arabidopsis thaliana: effects on phenotype, lignins and cell wall degradability.

Cinnamoyl CoA reductase (CCR; EC 1.2.1.44) is the first enzyme specific to the biosynthetic pathway leading to monolignols. Arabidopsis thaliana (L.) Heynh. plants transformed with a vector containing a full-length AtCCR1 cDNA in an antisense orientation were obtained and characterized. The most severely down-regulated homozygous plants showed drastic alterations to their phenotypical features. These plants had a 50% decrease in lignin content accompanied by changes in lignin composition and structure, with incorporation of ferulic acid into the cell wall. Microscopic analyses coupled with immunolabelling revealed a decrease in lignin deposition in normally lignified tissues and a dramatic loosening of the secondary cell wall of interfascicular fibers and vessels. Evaluation of in vitro digestibility demonstrated an increase in the enzymatic degradability of these transgenic lines. In addition, culture conditions were shown to play a substantial role in lignin level and structure in the wild type and in the effects of AtCCR1 repression efficiency.