ABSTRACT
The hippocampus receives an extensive cholinergic input from the medial septal nucleus that ramifies throughout all layers and plays a pivotal modulatory role in cognitive function. Although the pharmacological effects of exogenous application of cholinergic agonists have been extensively studied in hippocampal neurons, much less is known about the effects of synaptically released acetylcholine (ACh). In this respect, most studies have focused on the cholinergic afferent input to pyramidal neurons that produces a characteristically slow depolarizing synaptic response mediated by activation of muscarinic ACh receptors (mAChRs). Here we report that cholinergic afferent stimulation also elicits atropine-sensitive synaptic potentials in hippocampal CA1 interneurons but, in contrast to synaptic responses in pyramidal neurons, these are highly diverse in waveform, although can still be classified into five distinct subtypes. The most common response type (i) 64% of cells) consisted of a slow sustained membrane potential depolarization. The other 36% of responses could be subdivided into responses comprising of (ii) a biphasic membrane potential change in which an initial slow hyperpolarization subsequently transforms into a slow depolarization (20%), (iii) a pure, slow hyperpolarization (13%), and (iv) an oscillatory response persisting for several seconds (2%). Interestingly, there were also interneurons totally insensitive to both synaptic and pharmacological cholinergic challenge. Morphological investigation of recorded cells revealed no obvious correlation between responsiveness to cholinergic afferent stimulation and dendritic and axonal arborization. The current study suggests that synaptic release of ACh results in a complex and differential mAChR-mediated modulation of cellular excitability within the hippocampal interneuron population.
Subject(s)
Excitatory Postsynaptic Potentials/physiology , Hippocampus/cytology , Hippocampus/physiology , Interneurons/physiology , Receptors, Muscarinic/physiology , Acetylcholine/physiology , Action Potentials/drug effects , Action Potentials/physiology , Animals , Atropine/pharmacology , Carbachol/pharmacology , Cell Shape/physiology , Cholinergic Agonists/pharmacology , Cholinesterase Inhibitors/pharmacology , Excitatory Postsynaptic Potentials/drug effects , Female , Interneurons/drug effects , Male , Muscarinic Antagonists/pharmacology , Neural Pathways , Organ Culture Techniques , Physostigmine/pharmacology , Rats , Rats, Wistar , Septal Nuclei/cytology , Septal Nuclei/physiology , gamma-Aminobutyric Acid/physiologyABSTRACT
Nicotinic acetylcholine receptors (nAChRs) regulate neuronal excitability within the CNS. To assess the possible modulatory influence of nAChRs on epileptiform activity, a range of nAChR ligands were applied during experimentally induced epileptiform activity in rat hippocampal slices. Bath application of the potassium channel blocker 4-aminopyridine (4AP; 10-50 microM) resulted in the development of spontaneous epileptiform bursting activity in area CA3 that consisted of short duration (257+/-15 ms) field events occurring regularly at a frequency of 0.4+/-0.02 Hz. Subsequent co-application of the selective nAChR agonists 1,1-dimethyl-4-phenyl-piperazinium iodide (DMPP; 0.3-300 microM), choline (0.01-3mM) and lobeline (3-30 microM) produced sustained and concentration-dependent increases in burst frequency with maximal frequency potentiation of 37+/-5%, 27+/-5% and 24+/-11%, respectively. DMPP (10-30 microM; n=31) also potentiated epileptiform bursting induced by reducing GABA(A) receptor-mediated synaptic transmission using 20 microM bicuculline or enhancing NMDA receptor-mediated excitation by lowering extracellular Mg(2+). Irrespective of the epileptiform model studied all nAChR agonist induced frequency potentiation was reversed upon washout of the agonist or co-application of one of the selective nAChR antagonists dihydro-beta-erythroidine (10-30 microM), mecamylamine (50-200 microM) or alpha-bungarotoxin (100 nM). These results provide compelling evidence that activation of nAChRs exacerbate epileptiform activity in the rat hippocampus.
Subject(s)
Action Potentials/physiology , Epilepsy/physiopathology , Hippocampus/physiopathology , Receptors, Nicotinic/physiology , 2-Amino-5-phosphonovalerate/pharmacology , 4-Aminopyridine , Action Potentials/drug effects , Analysis of Variance , Animals , Bicuculline , Choline/pharmacology , Dose-Response Relationship, Drug , Drug Interactions , Electrophysiology/methods , Epilepsy/chemically induced , Excitatory Amino Acid Antagonists/pharmacology , Hippocampus/drug effects , In Vitro Techniques , Male , Nicotinic Agonists/pharmacology , Nicotinic Antagonists/pharmacology , Rats , Rats, Wistar , Regression AnalysisABSTRACT
AIMS: To investigate the effect of a pneumatic tube system (PTS) on the results of samples sent for blood gas analysis to a central laboratory. METHODS: Blood gas samples were analysed immediately or sent via the PTS to the laboratory for analysis. In addition, samples sent via the PTS in a pressure sealed container were compared with those sent non-pressure sealed to the laboratory. RESULTS: Samples sent via the PTS had significant alterations in their pO(2) values, which were not seen when samples were carried by hand to the laboratory. There was no effect on pCO(2) and pH values. The use of a pressure sealed container abolished the alteration in pO(2) values seen. CONCLUSIONS: Samples for blood gas analysis should be transported via a PTS using a pressure sealed container to avoid artefacts in the pO(2).
Subject(s)
Blood Specimen Collection/methods , Carbon Dioxide/blood , Oxygen/blood , Transportation/methods , Artifacts , Blood Chemical Analysis/methods , Humans , Hydrogen-Ion Concentration , Partial Pressure , Pressure , Reproducibility of ResultsABSTRACT
RATIONALE: Psychological dependence to the opioid analgesic morphine is attributable to the rewarding properties of the drug, and its evolution can be divided into two distinct phases: development and maintenance. Both phases can be studied using conditioned place preference (CPP). OBJECTIVES: To determine whether the two phases can be influenced by pre-treatment with gabapentin-like compounds. METHODS: CPP to morphine was used to demonstrate the rewarding properties of morphine in the presence or absence of gabapentin-like compounds. In-vivo microdialysis in the nucleus accumbens was used to determine the effects of gabapentin or pregabalin on morphine-induced dopamine release. RESULTS: Pretreatment with either gabapentin (10-100 mg/kg p.o.) or pregabalin (3-30 mg/kg p.o.) attenuated CPP induced by a submaximal dose of morphine (0.75 mg/kg). Neither gabapentin nor pregabalin had any effect alone in the CPP test. Both gabapentin-like compounds blocked the effect of morphine (0.75 mg/kg s.c.) to increase the release of dopamine in the nucleus accumbens. Studies of the maintenance of CPP to morphine showed CPP was maintained for at least 4 days after the initial test. In a second experiment, it was found that pregabalin (injected once, 24 h after CPP had been demonstrated) was able to reverse morphine-induced CPP. CONCLUSIONS: Neither gabapentin nor pregabalin induced CPP, but both compounds blocked the development of CPP to morphine and also blocked morphine's effects on dopamine release. Furthermore, pregabalin blocked the maintenance of morphine-induced CPP. It is concluded that gabapentin-like compounds, which have no intrinsic rewarding properties, may have some therapeutic use in the treatment of opioid dependence.