ABSTRACT
No increase in the number of chromosome aberrations in the bone marrow cells of the Wistar rats was observed on the 1st, 2nd, 7th and 15th days after epidermal and intradermal immunization by tularemic live vaccine. Subcutaneous injection of great quantities of tularemic microbic cells which were not used in practice increased the number of cells with chromosome aberrations only on the second day.
Subject(s)
Bacterial Vaccines/toxicity , Francisella tularensis/immunology , Mutagens , Animals , Bone Marrow/drug effects , Bone Marrow/ultrastructure , Chromosome Aberrations , Female , Immunization , Male , Metaphase/drug effects , Rats , Rats, Inbred Strains , Time FactorsABSTRACT
Cytogenetic disturbances due to cytostatic preparations--cyclophosphamide and methylnitrosourea--applied as single or ten-times administrations in different doses have been studied for rats immunized with tularemia live dried vaccine. It is established that with the application of these preparations to immunized animals the quantity of chromosomal aberrations in myelokaryocytes decreased from 1.4 to 2.2 times, compared with that in non-immunized animals.
Subject(s)
Bacterial Vaccines/immunology , Chromosome Aberrations , Francisella tularensis/immunology , Immunization , Animals , Antineoplastic Agents , Bone Marrow/drug effects , Bone Marrow/ultrastructure , Cyclophosphamide , Dose-Response Relationship, Drug , Female , Male , Metaphase/drug effects , Methylnitrosourea , Rats , Rats, Inbred StrainsABSTRACT
It is established that rat immunization with tularemia living vaccine inhibits Pliss lymphosarcoma growth and increases the life duration to tumour bearing animals. In tests with Zajdela ascitic hepatoma in 1/3 of the immunized animals ascites were not formed and the rats lived 150 days before they were killed.
Subject(s)
Bacterial Vaccines/immunology , Francisella tularensis/immunology , Immunization/methods , Neoplasms, Experimental/immunology , Animals , Liver Neoplasms, Experimental/immunology , Liver Neoplasms, Experimental/mortality , Liver Neoplasms, Experimental/prevention & control , Lymphoma, Non-Hodgkin/immunology , Lymphoma, Non-Hodgkin/mortality , Lymphoma, Non-Hodgkin/prevention & control , Neoplasm Transplantation , Neoplasms, Experimental/mortality , Neoplasms, Experimental/prevention & control , Phagocytes/immunology , Rats , Rats, Inbred Strains , Time FactorsABSTRACT
The influence of immunization with tularemic live dry vaccine applied singularly to Wistar rats skin on mutagenicity induced by cyclophosphamide, methylnitrosourea, benzo (a) pyrene, chloroprene and tetrachlorbutane was studied. The chemical compounds indicated were injected intraperitoneally at doses equal to one fifth of LD50 in 15 days after vaccination. The same doses of these chemicals were applied to intact rats (controls). Metaphase plates were prepared from rat bone marrows by known methods, 9600 metaphase cells were studied in total. The analysis showed that cytogenetic disturbances in immunized animals were significantly decreased (from 1.5 to 2.6 times), as compared to nonimmunized animals. The possible mechanisms of reducing the quantity of cytogenetic disorders in myelokaryocytes of immunized rats are discussed in this paper. The results obtained may be both of scientific and practical significance.
Subject(s)
Bacterial Vaccines/immunology , Francisella tularensis/immunology , Mutagens/pharmacology , Animals , Bone Marrow/drug effects , Carcinogens/pharmacology , Chromosome Aberrations , Female , Immunization , Male , Metaphase/drug effects , Mice , Mutation , Rats , Rats, Inbred Strains , Time FactorsABSTRACT
The paper deals with a study of the effect of a single challenge with living tularemia vaccine on the growth of such transplantable tumors as Zajdela's hepatoma, Pliss' lymphosarcoma, Walker's carcinosarcoma, Schwetz' erythromyelosis and sarcoma 45 in Wistar rats. Immunization was followed by a significant (37.2-86%) inhibition of the rate of growth of the said tumors. Ascites was detected in half the vaccinated animals of Zajdela's hepatoma series, this being matched by 100% in controls.
Subject(s)
Bacterial Vaccines/therapeutic use , Francisella tularensis/immunology , Neoplasms, Experimental/therapy , Animals , Female , Immunization , Male , Neoplasm Transplantation , Rats , Rats, Inbred StrainsABSTRACT
Chloroprene (2-chlorobutidiene 1,3) is an intermediate chemical product derived in the process of obtaining synthetic rubber. Chloroprene was shown by the experiments on mice and rats to be not carcinogenic. The application of chloroprene in animals by skin smears, subcutaneous injections, per os and intratracheal administration failed to induce any tumors in the experimental animals. No tumors were observed also in animals after using its combination with very small doses of dimethylbenz (a) anthracene.
