ABSTRACT
Patients with psoriasis and chronic hepatitis C virus (HCV) infection are a therapeutic challenge. Systemic psoriasis treatment with methotrexate and acitretin can be hepatotoxic, and interferon (IFN)-alpha for treatment of HCV can worsen psoriasis. Etanercept can be successfully used in patients with psoriasis and HCV. To our knowledge, this is the first case report of etanercept used prophylactically to prevent a psoriatic flare in a patient with HCV treated with IFN-alpha and ribavirin.
Subject(s)
Hepatitis C, Chronic/drug therapy , Immunoglobulin G/therapeutic use , Interferon-alpha/adverse effects , Psoriasis/prevention & control , Receptors, Tumor Necrosis Factor/therapeutic use , Ribavirin/adverse effects , Antiviral Agents/adverse effects , Antiviral Agents/therapeutic use , Drug Eruptions/etiology , Drug Eruptions/prevention & control , Drug Therapy, Combination , Etanercept , Humans , Interferon-alpha/therapeutic use , Male , Middle Aged , Psoriasis/chemically induced , Ribavirin/therapeutic useABSTRACT
HPLC and gel permeation chromatographic (GPC) characterization of complex phenol-formaldehyde resins is described. Reversed-phase HPLC fingerprints the phenolic monomers, dimers and some oligomers. The molecular masses of these phenolic compounds were determined using an ion trap mass spectrometer. GPC analyzes tetrahydrofuran-soluble phenolic polymers beyond HPLC capability. The molecular mass distribution and structural information of the phenolics was determined by both conventional and laser light-scattering calibration methods. GPC with both UV and refractive index detection provides weight concentration of phenolic resin and the molar concentration of the phenol unit in the oligomers or polymers.
Subject(s)
Chromatography, Gel/methods , Chromatography, High Pressure Liquid/methods , Phenols/isolation & purification , Spectrum Analysis/methods , Molecular Weight , Phenols/chemistryABSTRACT
A fluorescence-labeled wheat germ agglutinin staining technique (R. K. Sizemore et al., Appl. Environ. Microbiol. 56:2245-2247, 1990) was modified and found to be effective for staining gram-positive, acidophilic mining bacteria. Bacteria identified by others as being gram positive through 16S rRNA sequence analyses, yet clustering near the divergence of that group, stained weakly. Gram-negative bacteria did not stain. Background staining of environmental samples was negligible, and pyrite and soil particles in the samples did not interfere with the staining procedure.
