ABSTRACT
OBJECTIVES: Intramedullary gliomas are rare tumors accounting for less than 4% of all primary central nervous system tumors. The aims of this retrospective multicenter study were to assess their natural outcome as well as management. METHODS AND MATERIALS: We studied 332 patients from 1984 to 2011. Histopathological examination revealed 72% ependymomas (94% were low grade tumors), 24% astrocytomas (29% were high grade tumors), 2.4% mixed gliomas and 1.7% oligodendrogliomas. RESULTS: The mean age at diagnosis was 42.4 years for ependymomas, with male predominance, versus 39.6 years for astrocytomas. Pain was the most common initial presentation. In 20% of cases, astrocytomas were biopsied alone, but more than 80% of ependymomas had surgical resection. Radiotherapy and chemotherapy were reserved for malignant tumors, especially if they were ependymomas. The 5-year survival rate was 76.8% for astrocytomas and 94.5% for ependymomas. Histology, functional status prior to surgery, and tumor grade are among the prognostic factors. CONCLUSION: Our study showed that surgical treatment of gliomas is well codified, at least for ependymomas, but adjuvant treatment continues to play a marginal role in the management even in astrocytomas, which are infiltrative tumors.
Subject(s)
Glioma/therapy , Spinal Cord Neoplasms/therapy , Adult , Female , Glioma/diagnosis , Glioma/pathology , Humans , Male , Retrospective Studies , Spinal Cord Neoplasms/diagnosis , Spinal Cord Neoplasms/pathologyABSTRACT
The diagnosis of facioscapulohumeral muscular dystrophy (FSHD) can be difficult due to its clinical variability and complex genetic cause. We present three challenging cases: one misdiagnosis of FSHD, one patient with FSHD resembling mitochondrial myopathy, and one patient with combined FSHD and limb girdle muscular dystrophy 2A. Detailed clinical and genetic evaluation, including 4qA/4qB allele determination, may be needed for the diagnosis of FSHD.
Subject(s)
Muscular Dystrophy, Facioscapulohumeral/diagnosis , Adult , Aged , Alleles , Chromosomes, Human, Pair 4 , Diagnosis, Differential , Diagnostic Errors , Gene Deletion , Gene Dosage , Humans , Male , Middle Aged , Mitochondrial Myopathies/diagnosis , Muscular Dystrophies, Limb-Girdle/complications , Muscular Dystrophy, Facioscapulohumeral/complications , Muscular Dystrophy, Facioscapulohumeral/genetics , Muscular Dystrophy, Facioscapulohumeral/pathology , Mutation , Polymorphism, Genetic , Tandem Repeat SequencesABSTRACT
The sole cytogenetic abnormalities encountered in two childhood anaplastic intracerebral ependymomas were an isodicentric chromosome 22 in one case and an unbalanced chromosome 22 translocation associated with a partial deletion in the other. Fluorescence in situ hybridization analysis showed that the common 22q arm loss did not involve the rhabdoid region but included the EWS and NF2 loci. These results, in conjunction with data in the literature, suggest that the most frequently recurrent genomic loss in ependymomas does not involve the proximal 22q11.2 chromosome region but is localized distally to the hSNF5/INI1 locus. A tumor-suppressor gene, independent of the NF2 gene, which seems to be exclusively involved in intramedullary spinal cord ependymomas, might be implicated in the genesis of these intracranial tumors.
Subject(s)
Brain Neoplasms/genetics , Chromosome Deletion , Chromosomes, Human, Pair 22 , Ependymoma/genetics , Child, Preschool , Female , Humans , In Situ Hybridization, Fluorescence , InfantABSTRACT
The correlation between cytogenetic and histopathological findings were analysed in 189 meningiomas. The tumors were classified according to increasing degrees of anaplasia. We observed normal karyotype or only monosomy 22 in grade 1 (benign) tumors, while in grade 3 (anaplastic) only 1.5% of karyotypes were normal. Grade 2 (atypical) and 3 (anaplastic) tumors showed complex structural abnormalities. Loss of chromosome 14 were only found in grade 3. In cases with complex structural rearrangements, fluorescence in situ hybridization technique (FISH) has been realized and permitted a best identification of abnormalities. In our series, five patients recurred. They presented chromosomal abnormalities. These complex karyotypes in recurrent meningiomas might indicate aggressive tumor characteristics. Our results indicate histolopathological and cytogenetics correlations might represent a prognostic factor in meningiomas.
Subject(s)
Meningeal Neoplasms/diagnosis , Meningioma/diagnosis , Adult , Aged , Aged, 80 and over , Chromosome Aberrations/diagnosis , Chromosome Aberrations/genetics , Chromosome Disorders , Female , Humans , In Situ Hybridization, Fluorescence , Karyotyping , Male , Meningeal Neoplasms/genetics , Meningioma/genetics , Middle AgedABSTRACT
We studied mitochondrial respiratory chain function in skeletal muscle taken from 27 patients with idiopathic Parkinson's disease (PD; 21 Dopa-treated PD patients and 6 de novo patients), 5 patients with multiple system atrophy (MSA) and from 43 age-matched controls in order to determine the occurrence of mitochondrial respiratory chain abnormalities in parkinsonian syndromes. In our control subjects, we found a significant age-related decrease in the activity of respiratory chain complex I. As compared to carefully age-matched control subjects, activity of complex (NADH:ubiquinone reductase) was significantly lower in muscle mitochondria from patients with PD and MSA and a mean remaining activity < 30% of controls was observed. Mean activities of complexes III (ubiquinol:cytochrome c reductase) and IV (cytochrome c oxidase) were also lower in PD patients than controls, but a low activity (remaining activity < 30% of controls) was observed in only 5 PD patients for complex I and III or I and IV. No deficit in complex II activity (succinate:ubiquinone reductase) was observed. Our results support the hypothesis of a wide-spread mitochondrial complex I deficiency in PD and MSA as compared to age-matched controls, who showed age-related deficiency. This deficit can be found in de novo PD patients as well as in treated patients. The observed respiratory enzyme chain deficiency could not be explained by the dose and duration of L-Dopa or dopaminergic agonist treatment, the severity of the disease, anxiety or depression since no significant correlation was found between these parameters and enzyme complexes activities.
Subject(s)
Brain Diseases/metabolism , Mitochondria, Muscle/metabolism , Muscle, Skeletal/metabolism , Nerve Degeneration , Oxygen Consumption , Parkinson Disease/metabolism , Adult , Aged , Aged, 80 and over , Atrophy , Brain Diseases/pathology , Electron Transport Complex I , Electron Transport Complex III/metabolism , Electron Transport Complex IV/metabolism , Histocytochemistry , Humans , Middle Aged , NAD(P)H Dehydrogenase (Quinone)/metabolism , NADH, NADPH Oxidoreductases/metabolism , Regression AnalysisABSTRACT
In six cases of meningiomas, a karyotype with monosomy 22 and telomeric associations has been observed. The histopathological and cytogenetic correlations showed that the tumors with these chromosomal abnormalities were of a fibroblastic type and presented a certain degree of anaplasia. The relationship between telomeric association and malignancy is discussed.
Subject(s)
Chromosomes, Human, Pair 22 , Chromosomes, Human , Meningeal Neoplasms/genetics , Meningioma/genetics , Monosomy , Telomere/physiology , Humans , Karyotyping , Meningeal Neoplasms/pathology , Meningioma/pathologyABSTRACT
The muscle biopsy performed in a 58-year-old woman with a myopathy involving pelvic girdle and lower limbs displayed unusual intermediate filament aggregates by light and electron microscopy. No cardiac involvement was detected. The filamentous aggregates selective for type 1 fibers were found in subsarcolemmal and intermyofibrillar areas closely related to Z bands. Immunohistochemical study by light and electron microscopy using polyclonal and monoclonal antibodies and avidin-biotin peroxidase method revealed that aggregates consisted of desmin filaments. Desmin positive material was unstained with vimentine antibodies.
