ABSTRACT
PIP: Contraceptive hormonal injectable action of medroxy-progesterone acetate ciprionate estradiol (MPACE) in the reproductive system of female rats was studied. The female rats were selected in 2 groups: A and B, castrated and non castrated, respectively, and according to dose numbers in 6 subgroups: A0, A1, A2, A3, A4, A5 and B0, B1, B2, B3, B4, B5. Karyometric studies of nuclei from uterine epithelial tunica mucosa endometrial glands, myometrium, exocervix and vaginal epithelium were made. For each subgroup females were selected and 3 slides analyzed for each 1, which means 9 observations per subgroup. From uterine epithelial tunica mucosa, endometrial glands, and myometrium kariometric, 20 nuclei were measured per slide, in a total of 60. When dealing with vaginal and exocervical epithelia, 120 nuclei were measured as both are stratified epithelia. Karyometric studies were made in 15,120 nuclei and the results statistically analyzed. Nearly a 2-fold increase in nuclear volume was observed in female rats at the estrual phase as compared with castrated female controls. A higher significant MPACE effect was found in cells covering uterine epithelial tunica mucosa, endometric glands, and myometrium. The MPACE action on the exocervix and vaginal cells was not significant. As statistically assessed, the MPACE combination showed a predominance of the estrogenic portion with an increase of nuclear volumes but this action was balanced by the progestagenic fraction, as mitoses were unusual.^ieng
