ABSTRACT
He leído con interés el artículo "Reacciones físicas y psicológicas de los estudiantes de medicina ante la disección cadavérica en el curso de Anatomía Humana de una universidad peruana" de Paredes Orué y otros, publicado en esta revista.1) Coincido con los objetivos del estudio, que evalúa las reacciones físicas y psicológicas que afectan a los estudiantes de medicina humana ante la disección de cadáveres. Sus autores tienen el mérito de evidenciar las mínimas o "nulas" reacciones que puede tener un estudiante ante dicha actividad en el Perú; sin embargo, considero los siguientes puntos como discutibles a priori(AU)
Subject(s)
Humans , Students, Medical , Unified Health System , Dissection , GoalsABSTRACT
An indirect enzyme-linked immunoabsorbent assay (ELISA) for Trypanosoma cruzi was developed using epimastigote secretion/excretion proteins (ESEA antigens) obtained from axenic culture supernatants. A panel of 120 serum samples from subjects with confirmed Chagas disease (n = 50), healthy controls (n = 50), and patients with other parasitic diseases (n = 20) was used to evaluate the new ESEA-based ELISA (ELISA(ESEA)). This new test had excellent sensitivity (98%) and acceptable specificity (88%). Cross-reactivity was observed largely in sera from subjects with Leishmania and Ascaris infections. Using Western blotting and epimastigotes from two distinct T. cruzi isolates, several polypeptide bands with molecular masses ranging from 50 to 220 kDa were detected in pooled chagasic sera. However, the band pattern for each isolate was different. These data suggest that an inexpensive and technically simple ELISA based on ESEA antigens is a promising new tool for the diagnosis of Chagas disease.
