The prevalence, virulence, and serogroups of Dichelobacter nodosus and prevalence of Fusobacterium necrophorum in footrot lesions of sheep and cattle in Morocco.

Background and Aim: Footrot is a contagious disease of ruminants leading to severe economic losses. This study aimed to estimate the prevalence, virulence, and serogroups of Dichelobacter nodosus and the prevalence of Fusobacterium necrophorum in footrot lesions of sheep and cattle. Materials and Methods: A total of 106 pathogenic lesion samples were taken from 74 sheep and 32 cattle exhibiting typical footrot lesions and were analyzed for the presence of D. nodosus and F. necrophorum by real-time polymerase chain reaction (PCR). Both virulence and serogroup were estimated for D. nodosus positive samples. Results: Among the 106 samples, 89 were positive by PCR for F. necrophorum, D. nodosus, or both. Dichelobacter nodosus was detected at a rate of 78.3% versus 28.3% for F. necrophorum. Virulent D. nodosus strains were detected in 67.5% of positive samples, with a higher rate in sheep (73.4%) than in cattle (47.4%). Benign D. nodosus strains were detected in 57.8% of samples, with a lower prevalence rate in sheep (50%) than in cattle (84.2%). The positive samples of D. nodosus revealed the presence of three dominant serogroups (D, H, I) and three minor serogroups (G, C, A) by serogroup-specific multiplex PCR. Conclusion: The findings provided information on the prevalence of D. nodosus and F. necrophorum strains in footrot lesions of sheep and cattle in some regions of Morocco, which will be useful for developing an effective autovaccine for the prevention of this disease in cattle and sheep in these regions.

Comparative evaluation of three capripoxvirus-vectored peste des petits ruminants vaccines.

Sheep and goat pox (SGP) with peste des petits ruminants (PPR) are transboundary viral diseases of small ruminants that cause huge economic losses. Recombinant vaccines that can protect from both infections have been reported as a promising solution for the future. SGP was used as a vector to express two structural proteins hemagglutinin or the fusion protein of PPRV. We compared immunity conferred by recombinant capripoxvirus vaccines expressing H or F or both HF. Safety and efficacy were evaluated in goats and sheep. Two vaccine doses were tested in sheep, 104.5TCDI50 in 1ml dose was retained for the further experiment. Results showed that the recombinant HF confers an earlier and stronger immunity against both SGP and PPR. This recombinant vaccine protect also against the disease in exposed and unexposed sheep. The potential Differentiating Infected from Vaccinated Animals of recombinant vaccines is of great advantage in any eradication program.

[An evaluation of the effectiveness of applying 16-slice computed tomography (CT) to coronary arteries in preoperative aortic valve replacement]. / Evaluation du scanner 16 coupes des artères coronaires dans le bilan préopératoire du rétrécissement valvulaire aortique.

UNLABELLED: An evaluation of the effectiveness of applying 16-slice Computed Tomography (CT) to coronary arteries in preoperative aortic valve replacement. PURPOSE: To evaluate the effectiveness of using 16-slice CT to diagnose a significant stenosis in coronary arteries in patients with severe aortic valve stenosis. MATERIAL AND METHODS: 50 patients were included in the study. After a medium contrast injection, CT images of the arteries were taken using 0.75 mm slices. We paired the images with an ECG. Segments smaller than 1.5 mm were discarded, and the results were compared to those from the coronary angiography. RESULTS: A satisfactory visualization of the coronary network was obtained for 80% (40/50) of the patients. For these 40 patients, 23 of the 29 patients without coronary stenosis were correctly classified but 4 of the 11 patients with coronary lesions were not recognized. The sensitivity of the multi-slice CT in detecting a least one significant coronary stenosis is 63.6%, the specificity 79.3%, positive predictive value 53.8% and negative predictive value 85.2%. CONCLUSION: the 16-slice CT is a relatively effective and minimally invasive tool to highlight before valve replacement significant coronary stenosis in arteries greater than 1.5 mm in diameter in patients with severe aortic valvular stenosis. CT technology is currently insufficient for diagnosis, but we hope that with advances in multi-slice CT engineering, its use will help patients avoid invasive coronary angiographies.