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1.
Eur J Biochem ; 268(2): 268-77, 2001 Jan.
Article in English | MEDLINE | ID: mdl-11168360

ABSTRACT

Diverting the antigen from its normal intracellular location to other compartments in an antibody-mediated way represents a mode of action for intracellular antibodies [Cardinale, A., Lener, M., Messina, S., Cattaneo, A. & Biocca, S. (1998) FEBS Lett., 439, 197-202; Lener, M., Horn, I.R., Cardinale, A., Messina, S., Nielsen, U.B., Rybak, S.M., Hoogenboom, H.R., Cattaneo, A. & Biocca, S. (2000) Eur J Biochem. 267, 1196-205]. In the case of p21Ras, the sequestration of the antigen in aggregated structures in the cytoplasm of transfected cells leads to the inhibition of its biological function. We have further investigated the intracellular fate of the antigen-antibody complex by analyzing the effect of proteasome inhibitors on the formation and the intracellular localization of the aggregates. Overexpression of anti-Ras scFv fragments or inhibition of proteasomes activity leads to the formation of large perinuclear aggresomes formed of ubiquitinated-scFv fragments in which p21Ras is sequestered and degraded in an antibody-mediated way. Disruption of microtubules by nocodazole completely abrogates the accumulation of scFv fragments in a single aggresome and induces the dispersion of these structures in the periphery of the cell. Cotransfection of the GFP-scFv with a myc-tagged ubiquitin and colocalization with specific anti-proteasome antibodies indicate the recruitment of exogenous ubiquitin and proteasomes to the newly formed aggresomes. Taken together these results suggest that the intracellular antigen-antibody complex is naturally addressed to the ubiquitin-proteasome pathway and that the mechanism of ubiquitination does not inhibit the antibody binding properties and the capacity to block the antigen function.


Subject(s)
Antigen-Antibody Complex/metabolism , Immunoglobulin Fragments/metabolism , Immunoglobulin Variable Region/metabolism , Proto-Oncogene Proteins p21(ras)/metabolism , Cysteine Endopeptidases/metabolism , Cytoplasm/metabolism , Cytoplasm/radiation effects , Cytoplasm/ultrastructure , Immunoglobulin Fragments/genetics , Immunoglobulin Variable Region/genetics , Multienzyme Complexes/metabolism , Proteasome Endopeptidase Complex , Recombinant Proteins/metabolism , Signal Transduction , Ubiquitins/metabolism
2.
Biophys Chem ; 83(3): 223-37, 2000 Jan 24.
Article in English | MEDLINE | ID: mdl-10647852

ABSTRACT

Using a competitive reconstitution assay, we measured the free energy spent in nucleosome formation of eight telomeric DNAs, differing in sequence and/or in length. The obtained values are in satisfactorily good agreement with those derived from a theoretical model that allows the calculation of the free energy of nucleosome formation on the basis of sequence-dependent DNA elasticity, using a statistical thermodynamic approach. Both theoretical and experimental evaluations show that telomeres are characterized by the highest free energies of nucleosome formation among all the DNA sequences so far studied. The free energy of nucleosome formation varies according to the different telomeric sequences and the length of the fragments. Theoretical analysis and experimental mapping by lambda exonuclease show that telomeric nucleosomes occupy multiple positions spaced every telomeric repeat. Sequence-dependent DNA elasticity appears as the main determinant of the stability of telomeric nucleosomes and their multiple translational positioning.


Subject(s)
DNA/chemistry , Nucleosomes/chemistry , Telomere/chemistry , Animals , Arabidopsis/genetics , Base Sequence , Bombyx/genetics , Chlamydomonas reinhardtii/genetics , DNA, Fungal/chemistry , DNA, Plant/chemistry , DNA, Protozoan/chemistry , Elasticity , Humans , Molecular Sequence Data , Nucleic Acid Conformation , Nucleosomes/genetics , Protein Biosynthesis , Saccharomyces cerevisiae/genetics , Telomere/genetics , Tetrahymena thermophila/genetics , Thermodynamics
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