ABSTRACT
Transplacental transfer of specific IgG antibodies was studied in 46 pairs of human immunodeficiency virus type 1 (HIV-1)-seropositive women and their neonates and in 53 pairs of healthy HIV-seronegative mothers and their newborns. Neonatal and maternal sera were assessed by nephelometry for total levels of serum IgG and by ELISA for IgG antibodies to herpes simplex virus (HSV), varicella-zoster virus (VZV), measles virus, tetanus toxoid, streptolysin O, and Streptococcus pneumoniae capsular antigens. Placental transfer of IgG antibodies to VZV, tetanus toxoid, measles, streptolysin O, and S. pneumoniae was decreased by maternal HIV infection. Maternal levels of total IgG had an independent effect on transfer of antibodies to HSV, VZV, measles, and S. pneumoniae. Neonatal antibody levels to tetanus toxoid, measles, and S. pneumoniae were significantly lower in the HIV group. Both maternal hypergammaglobulinemia and maternal HIV infection may contribute to these low antibody levels at birth and thus lead to early infection in this high-risk population.
Subject(s)
HIV Infections/immunology , HIV Seropositivity/immunology , HIV-1 , Immunity, Maternally-Acquired/immunology , Immunoglobulin G/blood , Antibodies, Bacterial/blood , Antibodies, Bacterial/immunology , Antibodies, Viral/blood , Antibodies, Viral/immunology , Bacterial Capsules/immunology , Bacterial Proteins , Female , Fetal Blood/immunology , Herpesvirus 3, Human/immunology , Humans , Infant, Newborn , Measles virus/immunology , Simplexvirus/immunology , Streptococcus pneumoniae/immunology , Streptolysins/immunology , Tetanus Toxoid/immunologyABSTRACT
Os autores relatam sua experiencia com o uso de programa de controle de qualidade para contadores hematologicos de multiplos canais assim como o metodo utilizado para a calibracao destes instrumentos. O programa baseia-se fundamentalmente na analise diaria das medias dos indices eritrocitarios (VCM, HCM e CHCM) obtidas das amostras analisadas. O programa, introduzido sem sobrecarga, de trabalho tecnico e sem custos adicionais, permite detectar variacoes da calibracao dos contadores antes que estas atinjam niveis clinicamente significativos
