Coccidial dispersion across New World marsupials: Klossiella tejerai Scorza, Torrealba & Dagert, 1957 (Apicomplexa: Adeleorina) from the Brazilian common opossum Didelphis aurita (Wied-Neuwied) (Mammalia: Didelphimorphia).

Klossiella tejerai Scorza, Torrealba & Dagert, 1957 is a primitive coccidian parasite reported from the New World marsupials Didelphis marsupialis (Linnaeus) and Marmosa demerarae (Thomas). The current work describes K. tejerai from the Brazilian common opossum Didelphis aurita (Wied-Neuwied) in Southeastern Brazil, evidencing the coccidial dispersion across opossums of the same family. The sporocysts recovered from urine samples were ellipsoidal, 20.4 × 12.7 µm, with sporocyst residuum composed of scattered spherules and c.13 sporozoites per sporocyst, with refractile bodies and nucleus. Macrogametes, microgametes, sporonts, sporoblasts/sporocysts were identified within parasitophorous vacuoles of epithelial cells located near the renal corticomedullary junction. Didelphis marsupialis should not have transmitted K. tejerai to D. aurita because they are not sympatric; however M. demerarae is sympatric with D. marsupialis and D. aurita. Therefore, D. aurita becomes the third host species for K. tejerai in South America.

Alterações clínicas e patológicas durantea infecção experimental com Eimeria acervulinaem frangos de corte suplementados com betaína / Clinical and pathological changes during the experimental infection with Eimeria acervulina in broiler chickens suplemented with betaine

Com o objetivo de se avaliar o efeito da betaína na patogenicidade de Eimeria acervulina, 420 pintos de corte Cobb foraminoculados experimentalmente com 2 x 105 oocistos esporulados e alocados em baterias num esquema de blocos ao acasocom cinco tratamentos e seis repetições, incluindo-se um controle positivo, um grupo tratado com o anticoccidiano salinomicinae promotor de crescimento, e mais três níveis de betaína como aditivo na ração a 0,05, 0,10 e 0,15 por cento. No 14o dia de vida as avesforam individualmente infectadas com 2 x 105 oocistos esporulados. A betaína não foi capaz de interferir positivamente naresposta clínica e patologia das aves durante a infecção por E. acervulina, no entanto foi capaz de aumentar a produção deoocistos. É provável que a betaína possa ser utilizada como aditivo na ração das aves em substituição aos medicamentosconvencionais salinomicina e penicilina G potássica, cujos efeitos são semelhantes na recuperação do hospedeiro frente àcoccidiose.

Purposing to investigate the betaine effect on biology and morphology of developmental stages of Eimeria acervulina, 420 Cobbbroiler chicks were experimentally inoculated with 2 x 105 sporulated oocysts and housed in battery cages in a block design withfive treatments and six replicates each, including a positive control, a group treated with the anticoccidial salinomycin plusantibiotic growth promoter plus three levels of betaine as additive in the feed 0.05, 0.10 and 0.15 percent. Betaine was not able to affectthe clinical response and pathology during the infection, but improved oocyst output, possibly due to the osmotic mechanisms.In fact betaine could replace salinomycin plus growth promoter as additive in the feed which effects is closer in the recovery ofthe host affected by coccidiosis.