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Proc Natl Acad Sci U S A ; 80(7): 1811-5, 1983 Apr.
Article in English | MEDLINE | ID: mdl-6572941

ABSTRACT

Thymosin beta 4, containing 43 amino acids and acetylated at the NH2 terminus, is synthesized in vitro in a rabbit reticulocyte lysate or in a yeast protein-synthesis system in the presence of mRNA from rat spleen. The product formed was identified as beta 4 by immunoprecipitation by a specific anti-beta 4 antiserum, comigration with authentic beta 4 in NaDodSO4/polyacrylamide gel electrophoresis and in HPLC, and identity of peptide fragments. The immunoprecipitable product generated in the wheat germ protein-synthesizing system emerged slightly ahead of beta 4 in HPLC and appeared to lack the NH2-terminal acetyl group. There was no evidence for formation of a larger polypeptide precursor of beta 4 in any of the three systems used. In sucrose density gradient centrifugation, the mRNA coding for beta 4 was recovered in the 7-8S mRNA fraction.


Subject(s)
Thymosin/genetics , Thymus Hormones/genetics , Animals , Cell-Free System , Protein Biosynthesis , RNA, Messenger/genetics , RNA, Messenger/isolation & purification , Rats , Spleen/physiology , Thymosin/biosynthesis , Thymosin/immunology
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