[Neutrophil motility in patients with early infection (rapid analysis using automatic image processing system, Magiscan)]. / Podvizhnost' neitrofilov u bol'nykh s ranevoi infektsiei (ékspress-analiz na osnove sistemy obrabotki izobrazhenii Magiskan).

The authors studied the dependence of blood neutrophil motility on the physical state of patients with infected wounds. Neutrophil motility was recorded and analyzed with the help of Magiscan-2a system of automatic image processing. A special software was developed for monitoring the movement of cells and measuring their displacements. Motility of each separate cell and a mean motility of the neutrophil population were determined on the glass in the microscope field (512 x 512 microns). Velocity of each neutrophil was shown to be nonuniform but approximately constant. Cell velocities in each probe were distributed in a continuous manner. Mean velocity of neutrophils in the probe was a stable index of the population motility. Decrease in neutrophil motility in the blood of patients with infected wounds was probably due to the adverse effect of serum factors. The application of the system of image processing makes it possible to carry out express analysis of neutrophil motility to estimate the efficiency of medical treatment.

[Method of automatic recording and analysis of leukocyte migration based on the image analysis system]. / Metod avtomaticheskoi registratsii i analiza migratsii leikotsitov na baze sistemy obrabotki izobrazhenii.

The method of automatic registration and of the analysis of neutrophils' mobility with the use of the Image Analysis System is described in this paper. The authors developed an algorithm of cells identification which makes it possible to carry out cadre-by-cadre observing the shift of each cell's centre of gravity with the time interval between cadres more than 15 seconds. This algorithm also allows to measure areas, perimeters and orientation of cells and their changes. Looking after the shifts of the cells' centres of gravity allows to describe the cells' paths in the form of the following dependences: path-by-time, shift-by-time, angle of vector of shift alteration. Observing a great number of cells at the same time allows to get true statistical picture of neutrophils' activity and distribution of cells' activity in population of neutrophils during only some minutes of registration. It is shown that each cell in the population has its own constant velocity.

[CO2 mass transfer and acid-base balance under conditions of muscular activity at sea level and in the mountains]. / Massoperenos CO2 i kislotno-shchelochnoe ravnovesie pri myshechnoi deiatel'nosti na ravnine i v gorakh.

The state of the blood acid-base balance and dynamics of carbonic acid gas mass transfer were studied in sportsmen at the sea level and in mountains. It is shown that at the sea level due to an intensive muscular activity large amounts of CO2 are formed and excreted; the mass transfer of this gas is multiply accelerated, simultaneously, a pronounced decompensated metabolic acidosis is observed which in some cases is complicated respiratory acidosis. The similar exercises in mountains are followed by a more pronounced disturbance in the acid-base balance and a more intensified mass-transfer of CO2. After 12-day acclimatization and training in mountains the buffer blood capacity increases, the metabolic acidosis under conditions of muscular activity is less pronounced.