Subject(s)
Intestinal Absorption/drug effects , Intestine, Large/drug effects , Peptides/pharmacology , Animals , Epithelium/drug effects , Epithelium/physiology , In Vitro Techniques , Intestinal Absorption/physiology , Intestine, Large/physiology , Membrane Potentials/drug effects , Membrane Potentials/physiology , Rana temporaria , Time FactorsABSTRACT
I.V. Infusion of bombesine after eating raw meat inhibited for 30-60 min the secretion of gastric juice and hydrochloric acid in dogs. Within 90-120 min of simultaneous infusion of pentagastrin and bombesine, the amount of secreted juice and its acidity decreased and then the secretion of gastric parietal cells increased. Simultaneous infusion of histamine and bombesine increased the response of gastric parietal cells during the whole experiment as compared with the histamine effect alone. Microapplication of bombesine into cerebral structures also decreased the secretory response of the parietal cells.
Subject(s)
Bombesin/analogs & derivatives , Bombesin/pharmacology , Gastric Acid/metabolism , Gastric Juice/drug effects , Pepsin A/drug effects , Animals , Dogs , Dose-Response Relationship, Drug , Gastric Acidity Determination , Gastric Juice/chemistry , Gastric Juice/metabolism , Histamine/pharmacology , Meat , Parietal Cells, Gastric/drug effects , Parietal Cells, Gastric/metabolism , Pentagastrin/pharmacology , Pepsin A/analysis , Pepsin A/metabolism , Stimulation, Chemical , Time FactorsABSTRACT
A convenient route of synthesis of amphibian bombesin and bombesin-like peptide alytesin was found. These tetradecapeptides were obtained by assembling the 1-5 and 6-14 fragments by means of DCC-HONB or mixed anhydrides methods. Structure of the tetradecapeptides was confirmed by high resolution NMR spectroscopy data. The bombesin and alytesin synthesized potently decrease body temperature and stimulate pancreatic juice secretion.
