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1.
Tsitol Genet ; 26(6): 21-9, 1992.
Article in Russian | MEDLINE | ID: mdl-1292157

ABSTRACT

The uniform distribution of satellite DNA II and IV has been revealed using in situ hybridization and differential staining in centromeric regions of autosomes. The sex chromosomes have not found such nucleotide blocks. There is only minor satellite IV block inside Y chromosome short arm. The Y chromosome has got some (TG)n enriched blocks distributed also among other parts of genome and one copy of sequences like human ZFY gene. The high repetitive fraction of bovine genomic DNA have not revealed RFLP. However, the difference has been found by blot hybridization between genomic organization of satellite IV in cattle and yak chromosomal DNA. Non-Mendelian distribution of some such nucleotide blocks has been obtained for interspecies crosses of cattle and yak.


Subject(s)
Cattle/genetics , Chromosomes/ultrastructure , Genetic Variation/genetics , Genome , Repetitive Sequences, Nucleic Acid/genetics , Animals , Chromosome Banding , DNA, Satellite/genetics , Female , In Situ Hybridization , Male , Species Specificity , Y Chromosome/ultrastructure
2.
Tsitologiia ; 32(6): 626-32, 1990.
Article in Russian | MEDLINE | ID: mdl-1700522

ABSTRACT

Cytochemical and molecular peculiarities of heterochromatic regions of bovine chromosomes have been studied, using specific fluorochrome staining induced decondensation, in situ hybridization, pretreatment of restriction enzymes. The heterochromatin of autosomes demonstrated a strong homogeneity. In chromosome Y two small specific heterochromatic regions were found lacking a long repeated tandem block of nucleotides enriched in GC base pairs and having no tandem block of Bkm repeats (10(4) b.p.). This class repeats are probably interspersed in the bovine genome. A rather seldom character of mammalian karyotypes is the absence of cytochemical heterochromatin in the X chromosome.


Subject(s)
Chromosomes/ultrastructure , Heterochromatin/ultrastructure , Mitosis , Animals , Azacitidine/pharmacology , Base Composition/drug effects , Cattle , Cells, Cultured/drug effects , Cells, Cultured/metabolism , Cells, Cultured/ultrastructure , Chromosome Banding/methods , Chromosomes/drug effects , Chromosomes/metabolism , DNA Restriction Enzymes/pharmacology , DNA, Satellite/drug effects , Heterochromatin/drug effects , Heterochromatin/metabolism , Histocytochemistry , Lymphocytes/drug effects , Lymphocytes/metabolism , Lymphocytes/ultrastructure , Mitosis/drug effects , Nucleic Acid Hybridization , Repetitive Sequences, Nucleic Acid/drug effects , X Chromosome/drug effects , X Chromosome/metabolism , X Chromosome/ultrastructure , Y Chromosome/drug effects , Y Chromosome/metabolism , Y Chromosome/ultrastructure
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