Deleterious functional consequences of perfluoroalkyl substances accumulation into the myelin sheath.

Exposure to persistent organic pollutants during the perinatal period is of particular concern because of the potential increased risk of neurological disorders in adulthood. Here we questioned whether exposure to perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS) could alter myelin formation and regeneration. First, we show that PFOS, and to a lesser extent PFOA, accumulated into the myelin sheath of postnatal day 21 (p21) mice, whose mothers were exposed to either PFOA or PFOS (20 mg/L) via drinking water during late gestation and lactation, suggesting that accumulation of PFOS into the myelin could interfere with myelin formation and function. In fact, PFOS, but not PFOA, disrupted the generation of oligodendrocytes, the myelin-forming cells of the central nervous system, derived from neural stem cells localised in the subventricular zone of p21 exposed animals. Then, cerebellar slices were transiently demyelinated using lysophosphatidylcholine and remyelination was quantified in the presence of either PFOA or PFOS. Only PFOS impaired remyelination, a deleterious effect rescued by adding thyroid hormone (TH). Similarly to our observation in the mouse, we also showed that PFOS altered remyelination in Xenopus laevis using the Tg(Mbp:GFP-ntr) model of conditional demyelination and measuring, then, the number of oligodendrocytes. The functional consequences of PFOS-impaired remyelination were shown by its effects using a battery of behavioural tests. In sum, our data demonstrate that perinatal PFOS exposure disrupts oligodendrogenesis and myelin function through modulation of TH action. PFOS exposure may exacerbate genetic and environmental susceptibilities underlying myelin disorders, the most frequent being multiple sclerosis.

Towards a humanized PPARγ reporter system for in vivo screening of obesogens.

Overeating and lack of exercise are major contributors to the current obesity epidemic, but environmental contaminants, or obesogens, are also considered to be potential actors. A common obesogen target is the Peroxisome Proliferator Activated Receptor Gamma (PPARγ). Screening for exogenous obesogens requires in vivo systems as many xenobiotics exert their effects through metabolites. We thus developed a humanized in vivo PPARγ reporter model, using Xenopus laevis larvae, a species possessing metabolic capacities comparable to mammals. A somatic transgenesis approach was used to co-express an expression vector for the human PPARγ protein simultaneously with one of a series of reporter vectors, each containing a PPARγ Response Element (PPRE)-eGFP sequence. Treatment of tadpoles with PPARγ agonists, antagonists or candidate obesogens, significantly modulated eGFP expression. Thus, the system provides a promising proof of principle for a sensitive and reliable humanized in vivo tool to screen both novel PPARγ drug ligands and potential endocrine disruptors or obesogens targeting this receptor.

Is thyroid hormone signaling relevant for vertebrate embryogenesis?

Classically, thyroid hormones (THs) have been primarily associated with postembryonic development (Tata, 1968), notably metamorphosis in anuran amphibians and flat fish. This period is parallel to the perinatal period in man and many marked developmental transitions in other species. As amply described in other chapters, metamorphosis is characterized by a peak of thyroxine (T(4)) and triiodothyronine (T(3)) that is synchronous with the metamorphic climax. In contrast, the developmental period that characterizes embryonic development prior to the significant production of TH by the endogenous thyroid gland has received little attention. Furthermore, the prevailing concepts of TH physiology during this period have been framed by two observations in amphibians and mammals: first, TRs are expressed, while circulating TH levels are much lower than those during metamorphosis and, second, extrapolating from the knowledge largely obtained from in vitro models, in the absence of TH, the aporeceptor represses target gene transcription during premetamorphic development. We propose to revisit both concepts in the light of accumulating data, first, on TH availability both in eggs and in embryos and, second, on the increasing knowledge of the complexity of TR and TH control of transcription.

Scaling the effective area of higher-order-mode erbium-doped fiber amplifiers.

We demonstrate scaling of the effective area of higher-order mode, Er-doped fiber amplifiers. Two Er-doped higher-order mode fibers, one with 3800 µm(2) A(eff) in the LP(0,11) mode, and one with 6000 µm(2) effective area in the LP(0,14) mode, are demonstrated. Output beam profiles show clean higher order modes, and S(2) imaging measurements show low extraneous higher order mode content. CW and pulsed amplifier experiments are reported. Nanosecond pulses are amplified to 0.5 mJ pulse energy with 0.5 MW peak power.

Cladding-pumped erbium-doped multicore fiber amplifier.

A cladding pumped multicore erbium-doped fiber amplifier for simultaneous amplification of 6 channels is demonstrated. Peak gain over 32 dB has been obtained at a wavelength of 1560 nm and the bandwidth measured at 20-dB gain was about 35 nm. Numerical modeling of cladding pumped multicore erbium-doped amplifier was also performed to study the properties of the amplifier. The results of experiment and simulation are found to be in good agreement.

Measuring higher-order modes in a low-loss, hollow-core, photonic-bandgap fiber.

We perform detailed measurements of the higher-order-mode content of a low-loss, hollow-core, photonic-bandgap fiber. Mode content is characterized using Spatially and Spectrally resolved (S2) imaging, revealing a variety of phenomena. Discrete mode scattering to core-guided modes are measured at small relative group-delays. At large group delays a continuum of surface modes and core-guided modes can be observed. The LP11 mode is observed to split into four different group delays with different orientations, with the relative orientations preserved as the mode propagates through the fiber. Cutback measurements allow for quantification of the loss of different individual modes. The behavior of the modes in the low loss region of the fiber is compared to that in a high loss region of the fiber. Finally, a new measurement technique is introduced, the sliding-window Fourier transform of high-resolution transmission spectra of hollow-core fibers, which displays the dependence of HOM content on both wavelength and group delay. This measurement is used to illustrate the HOM content as function of coil diameter.

Thyroid hormone signaling in the Xenopus laevis embryo is functional and susceptible to endocrine disruption.

Thyroid hormone (TH) is essential for vertebrate brain development. Most research on TH and neuronal development focuses on late development, mainly the perinatal period in mammals. However, in human infants neuromotor development correlates best with maternal TH levels in the first trimester of pregnancy, suggesting that TH signaling could affect early brain development. Studying TH signaling in early embryogenesis in mammals is experimentally challenging. In contrast, free-living embryos, such as Xenopus laevis, permit physiological experimentation independent of maternal factors. We detailed key elements of TH signaling: ligands, receptors (TR), and deiodinases during early X. laevis development, before embryonic thyroid gland formation. Dynamic profiles for all components were found. Between developmental stages 37 and 41 (~48 h after hatching, coincident with a phase of continuing neurogenesis) significant increases in T(3) levels as well as in mRNA encoding deiodinases and TR occurred. Exposure of embryos at this developmental stage for 24 h to either a TH antagonist, NH-3, or to tetrabromobisphenol A, a flame retardant and known TH disruptor, differentially modulated the expression of a number of TH target genes implicated in neural stem cell function or neural differentiation. Moreover, 24-h exposure to either NH-3 or tetrabromobisphenol A diminished cell proliferation in the brain. Thus, these data show first, that TH signaling exerts regulatory roles in early X. laevis neurogenesis and second, that this period represents a potential window for endocrine disruption.

Coupled high Q-factor surface nanoscale axial photonics (SNAP) microresonators.

We experimentally demonstrate series of identical two, three, and five coupled high Q-factor surface nanoscale axial photonics (SNAP) microresonators formed by periodic nanoscale variation of the optical fiber radius. These microresonators are fabricated with a 100 µm period along an 18 µm radius optical fiber. The axial FWHM of these microresonators is 80 µm and their Q-factor exceeds 10(7). In addition, we demonstrate a SNAP microresonator with the axial FWHM as small as 30 µm and the axial FWHM of the fundamental mode as small as 10 µm. These results may potentially enable the dense integration of record low loss coupled photonic microdevices on the optical fiber platform.

WDM/SDM transmission of 10 x 128-Gb/s PDM-QPSK over 2688-km 7-core fiber with a per-fiber net aggregate spectral-efficiency distance product of 40,320 km·b/s/Hz.

We demonstrate 2688-km multi-span transmission using wavelength-division multiplexing (WDM) of ten 50-GHz spaced 128-Gb/s PDM-QPSK signals, space-division multiplexed (SDM) in a low-crosstalk 76.8-km seven-core fiber, achieving a record net aggregate per-fiber-spectral-efficiency-distance product of 40,320 km·b/s/Hz. The demonstration was enabled by a novel core-to-core signal rotation scheme implemented in a 7-fold, synchronized recirculating loop apparatus.

Surface nanoscale axial photonics: robust fabrication of high-quality-factor microresonators.

Recently introduced surface nanoscale axial photonics (SNAP) makes it possible to fabricate high-Q-factor microresonators and other photonic microdevices by dramatically small deformation of the optical fiber surface. To become a practical and robust technology, the SNAP platform requires methods enabling reproducible modification of the optical fiber radius at nanoscale. In this Letter, we demonstrate superaccurate fabrication of high-Q-factor microresonators by nanoscale modification of the optical fiber radius and refractive index using CO2 laser and UV excimer laser beam exposures. The achieved fabrication accuracy is better than 2 Å in variation of the effective fiber radius.

112-Tb/s space-division multiplexed DWDM transmission with 14-b/s/Hz aggregate spectral efficiency over a 76.8-km seven-core fiber.

We describe a new multicore fiber (MCF) having seven single-mode cores arranged in a hexagonal array, exhibiting low crosstalk among the cores and low loss across the C and L bands. We experimentally demonstrate a record transmission capacity of 112 Tb/s over a 76.8-km MCF using space-division multiplexing and dense wavelength-division multiplexing (DWDM). Each core carries 160 107-Gb/s polarization-division multiplexed quadrature phase-shift keying (PDM-QPSK) channels on a 50-GHz grid in the C and L bands, resulting in an aggregate spectral efficiency of 14 b/s/Hz. We further investigate the impact of the inter-core crosstalk on a 107-Gb/s PDM-QPSK signal after transmitting through the center core of the MCF when all the 6 outer cores carry same-wavelength 107-Gb/s signals with equal powers, and discuss the system implications of core-to-core crosstalk on ultra-long-haul transmission.

Amplification and noise properties of an erbium-doped multicore fiber amplifier.

A multicore erbium-doped fiber (MC-EDF) amplifier for simultaneous amplification in the 7-cores has been developed, and the gain and noise properties of individual cores have been studied. The pump and signal radiation were coupled to individual cores of MC-EDF using two tapered fiber bundled (TFB) couplers with low insertion loss. For a pump power of 146 mW, the average gain achieved in the MC-EDF fiber was 30 dB, and noise figure was less than 4 dB. The net useful gain from the multicore-amplifier, after taking into consideration of all the passive losses, was about 23-27 dB. Pump induced ASE noise transfer between the neighboring channel was negligible.

1.12-Tb/s 32-QAM-OFDM superchannel with 8.6-b/s/Hz intrachannel spectral efficiency and space-division multiplexed transmission with 60-b/s/Hz aggregate spectral efficiency.

We demonstrate the generation of a 1.12-Tb/s superchannel based on coherent optical orthogonal frequency-division multiplexing with polarization-division multiplexed 32-QAM subcarriers, achieving a net intrachannel-spectral-efficiency (ISE) of 8.6 b/s/Hz. Using space-division multiplexing (SDM), we transmit this superchannel over a 76.8-km low-crosstalk multi-core-fiber (MCF) with a record aggregate ISE of 60 b/s/Hz per fiber. We also discuss the impact of core-to-core crosstalk on transmission performance, as well as future perspectives of MCF-based SDM transmission.

Surface nanoscale axial photonics.

Dense photonic integration promises to revolutionize optical computing and communications. However, efforts towards this goal face unacceptable attenuation of light caused by surface roughness in microscopic devices. Here we address this problem by introducing Surface Nanoscale Axial Photonics (SNAP). The SNAP platform is based on whispering gallery modes circulating around the optical fiber surface and undergoing slow axial propagation readily described by the one-dimensional Schrödinger equation. These modes can be steered with dramatically small nanoscale variation of the fiber radius, which is quite simple to introduce in practice. Extremely low loss of SNAP devices is achieved due to the low surface roughness inherent in a drawn fiber surface. In excellent agreement with the developed theory, we experimentally demonstrate localization of light in quantum wells, halting light by a point source, tunneling through potential barriers, dark states, etc. This demonstration has intriguing potential applications in filtering, switching, slowing light, and sensing.

A higher-order-mode erbium-doped-fiber amplifier.

We demonstrate the first erbium-doped fiber amplifier operating in a single, large-mode area, higher-order mode. A high-power, fundamental-mode, Raman fiber laser operating at 1480 nm was used as a pump source. Using a UV-written, long-period grating, both pump and 1564 nm signal were converted to the LP(0,10) mode, which had an effective area of 2700 microm(2) at 1550 nm. A maximum output power of 5.8 W at 1564 nm with more than 20 dB of gain in a 2.68 m long amplifier was obtained. The mode profile was undistorted at the highest output power.

Seven-core multicore fiber transmissions for passive optical network.

We design and fabricate a novel multicore fiber (MCF), with seven cores arranged in a hexagonal array. The fiber properties of MCF including low crosstalk, attenuation and splice loss are described. A new tapered MCF connector (TMC), showing ultra-low crosstalk and losses, is also designed and fabricated for coupling the individual signals in-and-out of the MCF. We further propose a novel network configuration using parallel transmissions with the MCF and TMC for passive optical network (PON). To the best of our knowledge, we demonstrate the first bi-directional parallel transmissions of 1310 nm and 1490 nm signals over 11.3-km of seven-core MCF with 64-way splitter for PON.

Coherence of supercontinua generated by ultrashort pulses compressed in optical fibers.

Femtosecond fiber lasers together with nonlinear fibers are compact, reliable, all-fiber supercontinuum sources. Maintaining an all-fiber configuration, however, necessitates pulse compression in an optical fiber, which can lead to nonlinearities for subhundred femtosecond, nanojoule pulses. In this work we show that using large-mode-area fibers for pulse compression mitigates the nonlinearity, resulting in compressed pulses with significantly reduced satellite pulses. Consequently, supercontinua generated with these pulses are shown to have as much as a 10 dB increase in coherence fringe contrast. By using a hybrid highly nonlinear fiber-photonic crystal fiber, the continuum can be extended to visible wavelengths while still maintaining high coherence.

Visible continuum generation using a femtosecond erbium-doped fiber laser and a silica nonlinear fiber.

Supercontinuum extending to visible wavelengths is generated in a hybrid silica nonlinear fiber pumped at 1560 nm by a femtosecond, erbium-doped fiber laser. The hybrid nonlinear fiber consists of a short length of highly nonlinear, germano-silicate fiber (HNLF) spliced to a length of photonic crystal fiber (PCF). A 2 cm length of HNLF provides an initial stage of continuum generation due to higher-order soliton compression and dispersive wave generation before launching into the PCF. The visible radiation is generated in the fundamental mode of the PCF.

Demonstration of bend-induced nonlinearities in large-mode-area fibers.

We present what we believe to be the first direct measurements of enhanced nonlinearities in large-mode-area fibers due to bend induced reductions in effective area. Both Raman scattering and self-phase modulation are observed to increase in tightly coiled fibers. The measured increase in nonlinearity compares well with predictions from simulations of the modal effective area.

SBS gain efficiency measurements and modeling in a 1714 mum(2) effective area LP(08) higher-order mode optical fiber.

The stimulated Brillouin scattering (SBS) gain efficiencies were measured in the LP(08) and LP(01) modes of a higher-order-mode optical fiber. Gain efficiencies C(B) of 0.0085 and 0.20 (m-W)(-1) were measured for the LP(08) and LP(01) modes at 1083 nm, respectively. C(B) is inversely proportional to the optical effective area Aeff and the same core-localized acoustic phonon seeds the SBS process in each case. An acoustic modal analysis and a distributed phenomenological model are presented to facilitate the data analysis and interpretation. The LP(08) mode exhibits a threshold powerlength product of 2.5 kW-m.