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1.
Nucleic Acids Res ; 45(10): e89, 2017 Jun 02.
Article in English | MEDLINE | ID: mdl-28204586

ABSTRACT

Delivery of DNA to cells and its subsequent integration into the host genome is a fundamental task in molecular biology, biotechnology and gene therapy. Here we describe an IP-free one-step method that enables stable genome integration into either prokaryotic or eukaryotic cells. A synthetic mariner transposon is generated by flanking a DNA sequence with short inverted repeats. When purified recombinant Mos1 or Mboumar-9 transposase is co-transfected with transposon-containing plasmid DNA, it penetrates prokaryotic or eukaryotic cells and integrates the target DNA into the genome. In vivo integrations by purified transposase can be achieved by electroporation, chemical transfection or Lipofection of the transposase:DNA mixture, in contrast to other published transposon-based protocols which require electroporation or microinjection. As in other transposome systems, no helper plasmids are required since transposases are not expressed inside the host cells, thus leading to generation of stable cell lines. Since it does not require electroporation or microinjection, this tool has the potential to be applied for automated high-throughput creation of libraries of random integrants for purposes including gene knock-out libraries, screening for optimal integration positions or safe genome locations in different organisms, selection of the highest production of valuable compounds for biotechnology, and sequencing.


Subject(s)
DNA Transposable Elements , DNA-Binding Proteins/genetics , Mutagenesis, Insertional , Plasmids/metabolism , Transposases/genetics , Base Sequence , Cloning, Molecular , DNA-Binding Proteins/metabolism , Electroporation , Escherichia coli/genetics , Escherichia coli/metabolism , Genes, Synthetic , HEK293 Cells , HeLa Cells , Humans , Inverted Repeat Sequences , Lipids/chemistry , Plasmids/chemistry , Sequence Analysis, DNA , Transfection , Transposases/metabolism
2.
J Biol Chem ; 290(21): 13531-40, 2015 May 22.
Article in English | MEDLINE | ID: mdl-25869132

ABSTRACT

The inverted repeat (IR) sequences delimiting the left and right ends of many naturally active mariner DNA transposons are non-identical and have different affinities for their transposase. We have compared the preferences of two active mariner transposases, Mos1 and Mboumar-9, for their imperfect transposon IRs in each step of transposition: DNA binding, DNA cleavage, and DNA strand transfer. A 3.1 Å resolution crystal structure of the Mos1 paired-end complex containing the pre-cleaved left IR sequences reveals the molecular basis for the reduced affinity of the Mos1 transposase DNA-binding domain for the left IR as compared with the right IR. For both Mos1 and Mboumar-9, in vitro DNA transposition is most efficient when the preferred IR sequence is present at both transposon ends. We find that this is due to the higher efficiency of cleavage and strand transfer of the preferred transposon end. We show that the efficiency of Mboumar-9 transposition is improved almost 4-fold by changing the 3' base of the preferred Mboumar-9 IR from guanine to adenine. This preference for adenine at the reactive 3' end for both Mos1 and Mboumar-9 may be a general feature of mariner transposition.


Subject(s)
DNA-Binding Proteins/chemistry , DNA-Binding Proteins/metabolism , DNA/metabolism , Drosophila/enzymology , Inverted Repeat Sequences/genetics , Plasmids/genetics , Transposases/chemistry , Transposases/metabolism , Adenine/chemistry , Animals , Base Sequence , Crystallography, X-Ray , DNA/genetics , DNA-Binding Proteins/genetics , Gene Expression Regulation, Enzymologic , Guanine/chemistry , Models, Molecular , Molecular Sequence Data , Protein Conformation , Transposases/genetics
3.
Biochemistry ; 53(4): 682-9, 2014 Feb 04.
Article in English | MEDLINE | ID: mdl-24404958

ABSTRACT

Most DNA transposons move from one genomic location to another by a cut-and-paste mechanism and are useful tools for genomic manipulations. Short inverted repeat (IR) DNA sequences marking each end of the transposon are recognized by a DNA transposase (encoded by the transposon itself). This enzyme cleaves the transposon ends and integrates them at a new genomic location. We report here a comparison of the biophysical and biochemical properties of two closely related and active mariner/Tc1 family DNA transposases: Mboumar-9 and Mos1. We compared the in vitro cleavage activities of the enzymes on their own IR sequences, as well as cross-recognition of their inverted repeat sequences. We found that, like Mos1, untagged recombinant Mboumar-9 transposase is a dimer and forms a stable complex with inverted repeat DNA in the presence of Mg(2+) ions. Mboumar-9 transposase cleaves its inverted repeat DNA in the manner observed for Mos1 transposase. There was minimal cross-recognition of IR sequences between Mos1 and Mboumar-9 transposases, despite these enzymes having 68% identical amino acid sequences. Transposases sharing common biophysical and biochemical properties, but retaining recognition specificity toward their own IR, are a promising platform for the design of chimeric transposases with predicted and improved sequence recognition.


Subject(s)
DNA-Binding Proteins/chemistry , Transposases/chemistry , Cations, Divalent , DNA/chemistry , DNA Cleavage , Inverted Repeat Sequences , Magnesium/chemistry , Plasmids , Protein Multimerization , Protein Stability , Recombinant Proteins/chemistry , Solutions , Temperature
4.
Curr Biol ; 22(11): R432-7, 2012 Jun 05.
Article in English | MEDLINE | ID: mdl-22677280

ABSTRACT

June 27, 1970 was a significant day for our understanding of both the flow of information in biological systems and the evolution of eukaryotic genomes as this was the day that Nature published back-to-back papers reporting the discovery of an enzyme that copies RNA into DNA. This soon became known as reverse transcriptase and the RNA tumour viruses in which it was detected were renamed retroviruses. The realisation that retroviruses can convert their genomic RNA into DNA provided a route by which they could integrate into the chromosomes of infected cells as Howard Temin and his colleagues had proposed some years earlier. At the time it was thought that the ability to copy RNA into DNA would be confined to retroviruses. One of the more startling outcomes of whole genome DNA sequencing has been the discovery that eukaryotes can have more reverse transcriptase genes than genes coding for any other protein, and that the largest single component of many eukaryotic genomes has been generated by reverse transcription.


Subject(s)
Retroelements/physiology , Animals , Evolution, Molecular , Humans , RNA-Directed DNA Polymerase/genetics , RNA-Directed DNA Polymerase/metabolism , Terminal Repeat Sequences
5.
RNA ; 18(4): 729-37, 2012 Apr.
Article in English | MEDLINE | ID: mdl-22345148

ABSTRACT

RNA localization is a key mechanism for targeting proteins to particular subcellular domains. Sequences necessary and sufficient for localization have been identified, but little is known about factors that affect its kinetics. Transcripts of gurken and the I factor, a non-LTR retrotransposon, colocalize at the nucleus in the dorso-antero corner of the Drosophila oocyte directed by localization signals, the GLS and ILS. I factor RNA localizes faster than gurken after injection into oocytes, due to a difference in the intrinsic localization ability of the GLS and ILS. The kinetics of localization of RNA containing the ILS are enhanced by the presence of a stem-loop, the A loop. This acts as an RNA:RNA interaction element in vivo and in vitro, and stimulates localization of RNA containing other localization signals. RNA:RNA interaction may be a general mechanism for modulating RNA localization and could allow an mRNA that lacks a localization signal to hitchhike on another RNA that has one.


Subject(s)
Drosophila/genetics , RNA/chemistry , Animals , Base Sequence , DNA Primers , Female
6.
Curr Biol ; 21(16): R630-2, 2011 Aug 23.
Article in English | MEDLINE | ID: mdl-21855004

ABSTRACT

The genome of Drosophila is protected from DNA damage during oogenesis by a mechanism involving short RNAs. Surprisingly transcription of these RNAs requires that their DNA is associated with a histone modification usually associated with gene silencing.


Subject(s)
DNA Transposable Elements , Drosophila melanogaster/genetics , Heterochromatin/metabolism , RNA, Small Interfering/biosynthesis , Animals
7.
Cell ; 138(6): 1096-108, 2009 Sep 18.
Article in English | MEDLINE | ID: mdl-19766564

ABSTRACT

A key step in cut-and-paste DNA transposition is the pairing of transposon ends before the element is excised and inserted at a new site in its host genome. Crystallographic analyses of the paired-end complex (PEC) formed from precleaved transposon ends and the transposase of the eukaryotic element Mos1 reveals two parallel ends bound to a dimeric enzyme. The complex has a trans arrangement, with each transposon end recognized by the DNA binding region of one transposase monomer and by the active site of the other monomer. Two additional DNA duplexes in the crystal indicate likely binding sites for flanking DNA. Biochemical data provide support for a model of the target capture complex and identify Arg186 to be critical for target binding. Mixing experiments indicate that a transposase dimer initiates first-strand cleavage and suggest a pathway for PEC formation.


Subject(s)
DNA Transposable Elements , DNA-Binding Proteins/metabolism , Drosophila/genetics , Transposases/metabolism , Animals , Catalytic Domain , Crystallography, X-Ray , DNA-Binding Proteins/chemistry , Models, Molecular , Protein Structure, Tertiary , Transposases/chemistry , X-Ray Diffraction
8.
Curr Biol ; 19(14): R555-8, 2009 Jul 28.
Article in English | MEDLINE | ID: mdl-19640491

ABSTRACT

Ciliate development requires assembly of functional genes from segments separated by intervening sequences now shown to have properties of transposons. This may be a relic of a time when transposition drove genome evolution, leading to the differentiation of the germline micronucleus and somatic macronucleus.


Subject(s)
Ciliophora/growth & development , Ciliophora/genetics , DNA Transposable Elements/genetics , Evolution, Molecular , Gene Expression Regulation/physiology , Genome, Protozoan/genetics , Animals , Macronucleus/physiology , Reproduction/physiology
9.
Curr Biol ; 18(13): R561-3, 2008 Jul 08.
Article in English | MEDLINE | ID: mdl-18606126

ABSTRACT

The Piwi-interacting RNA interference pathway plays an important role in suppressing transposable elements in the Drosophila germline. Now, deep sequencing of short RNAs from somatic tissue and cell culture has identified a novel class of endogenous siRNAs that may have a similar role in the soma.


Subject(s)
DNA Transposable Elements , Drosophila/metabolism , RNA Interference , RNA, Small Interfering/metabolism , Animals , Argonaute Proteins , Drosophila Proteins , Proteins/metabolism , RNA-Induced Silencing Complex
10.
Nucleic Acids Res ; 36(8): 2522-9, 2008 May.
Article in English | MEDLINE | ID: mdl-18319284

ABSTRACT

Penelope-like elements (PLEs) represent a new class of retroelements identified in more than 80 species belonging to at least 10 animal phyla. Penelope isolated from Drosophila virilis is the only known transpositionally active representative of this class. Although the size and structure of the Penelope major transcript has been previously described in both D. virilis and D. melanogaster transgenic strains, the architecture of the Penelope regulatory region remains unknown. In order to determine the localization of presumptive Penelope promoter and enhancer-like elements, segments of the putative Penelope regulatory region were linked to a CAT reporter gene and introduced into D. melanogaster by P-element-mediated transformation. The results obtained using ELISA to measure CAT expression levels and RNA studies, including RT-PCR, suggest that the active Penelope transposon contains an internal promoter similar to the TATA-less promoters of LINEs. The results also suggest that some of the Penelope regulatory sequences control the preferential expression in the ovaries of the adult flies by enhancing expression in the ovary and reducing expression in the carcass. The possible significance of the intron within Penelope for the function and evolution of PLEs, and the effect of Penelope insertions on adjacent genes, are discussed.


Subject(s)
Drosophila/genetics , Gene Expression Regulation , Promoter Regions, Genetic , Retroelements , Animals , Animals, Genetically Modified , Base Sequence , Drosophila melanogaster/genetics , Enhancer Elements, Genetic , Female , Genes, Reporter , Molecular Sequence Data , Ovary/metabolism , RNA, Messenger/analysis , Sequence Homology, Nucleic Acid
11.
Article in English | MEDLINE | ID: mdl-17565190

ABSTRACT

A complex formed between Mos1 transposase and its inverted-repeat DNA has been crystallized. The crystals diffract to 3.25 A resolution and exhibit monoclinic (P2(1)) symmetry, with unit-cell parameters a = 120.8, b = 85.1, c = 131.6 A, beta = 99.3 degrees . The X-ray diffraction data display noncrystallographic twofold symmetry and characteristic dsDNA diffraction at approximately 3.3 A. Biochemical analyses confirmed the presence of DNA and full-length protein in the crystals. The relationship between the axis of noncrystallographic symmetry, the unit-cell axes and the DNA diffraction pattern are discussed. The data are consistent with the previously proposed model of the paired-ends complex containing a dimer of the transposase.


Subject(s)
DNA-Binding Proteins/chemistry , Nucleic Acid Conformation , Transposases/chemistry , Base Sequence , Chromatography, High Pressure Liquid , Crystallization , Crystallography, X-Ray , DNA Primers , Electrophoresis, Polyacrylamide Gel
12.
BMC Dev Biol ; 7: 52, 2007 May 21.
Article in English | MEDLINE | ID: mdl-17517128

ABSTRACT

BACKGROUND: Movement of cells, either as amoeboid individuals or in organised groups, is a key feature of organ formation. Both modes of migration occur during Drosophila embryonic gonad development, which therefore provides a paradigm for understanding the contribution of these processes to organ morphogenesis. Gonads of Drosophila are formed from three distinct cell types: primordial germ cells (PGCs), somatic gonadal precursors (SGPs), and in males, male-specific somatic gonadal precursors (msSGPs). These originate in distinct locations and migrate to associate in two intermingled clusters which then compact to form the spherical primitive gonads. PGC movements are well studied, but much less is known of the migratory events and other interactions undergone by their somatic partners. These appear to move in organised groups like, for example, lateral line cells in zebra fish or Drosophila ovarian border cells. RESULTS: We have used time-lapse fluorescence imaging to characterise gonadal cell behaviour in wild type and mutant embryos. We show that the homeodomain transcription factor Six4 is required for the migration of the PGCs and the msSGPs towards the SGPs. We have identified a likely cause of this in the case of PGCs as we have found that Six4 is required for expression of Hmgcr which codes for HMGCoA reductase and is necessary for attraction of PGCs by SGPs. Six4 affects msSGP migration by a different pathway as these move normally in Hmgcr mutant embryos. Additionally, embryos lacking fully functional Six4 show a novel phenotype in which the SGPs, which originate in distinct clusters, fail to coalesce to form unified gonads. CONCLUSION: Our work establishes the Drosophila gonad as a model system for the analysis of coordinated cell migrations and morphogenesis using live imaging and demonstrates that Six4 is a key regulator of somatic cell function during gonadogenesis. Our data suggest that the initial association of SGP clusters is under distinct control from the movements that drive gonad compaction.


Subject(s)
Cell Movement/genetics , Drosophila Proteins/genetics , Drosophila/genetics , Germ Cells/cytology , Gonads/embryology , Homeodomain Proteins/genetics , Nerve Tissue Proteins/genetics , Transcription Factors/genetics , Animals , Drosophila/embryology , Embryo, Nonmammalian , Gene Expression Regulation, Developmental , Immunohistochemistry , Microscopy, Fluorescence , Organogenesis/genetics
14.
Dev Biol ; 294(1): 220-31, 2006 Jun 01.
Article in English | MEDLINE | ID: mdl-16595131

ABSTRACT

Patterning of the Drosophila embryonic mesoderm requires the regulation of cell type-specific factors in response to dorsoventral and anteroposterior axis information. For the dorsoventral axis, the homeodomain gene, tinman, is a key patterning mediator for dorsal mesodermal fates like the heart. However, equivalent mediators for more ventral fates are unknown. We show that D-six4, which encodes a Six family transcription factor, is required for the appropriate development of most cell types deriving from the non-dorsal mesoderm - the fat body, somatic cells of the gonad, and a specific subset of somatic muscles. Misexpression analysis suggests that D-Six4 and its likely cofactor, Eyes absent, are sufficient to impose these fates on other mesodermal cells. At stage 10, the mesodermal expression patterns of D-six4 and tin are complementary, being restricted to the dorsal and non-dorsal regions respectively. Our data suggest that D-six4 is a key mesodermal patterning mediator at this stage that regulates a variety of cell-type-specific factors and hence plays an equivalent role to tin. At stage 9, however, D-six4 and tin are both expressed pan-mesodermally. At this stage, tin function is required for full D-six4 expression. This may explain the known requirement for tin in some non-dorsal cell types.


Subject(s)
Body Patterning , Drosophila Proteins/physiology , Homeodomain Proteins/physiology , Mesoderm/cytology , Nerve Tissue Proteins/physiology , Transcription Factors/physiology , Animals , Drosophila Proteins/genetics , Drosophila melanogaster/embryology , Embryo, Nonmammalian , Eye Proteins/genetics , Gene Expression Regulation, Developmental , Muscles , Repressor Proteins/genetics , Trans-Activators/genetics
15.
EMBO J ; 25(6): 1324-34, 2006 Mar 22.
Article in English | MEDLINE | ID: mdl-16511570

ABSTRACT

We present the crystal structure of the catalytic domain of Mos1 transposase, a member of the Tc1/mariner family of transposases. The structure comprises an RNase H-like core, bringing together an aspartic acid triad to form the active site, capped by N- and C-terminal alpha-helices. We have solved structures with either one Mg2+ or two Mn2+ ions in the active site, consistent with a two-metal mechanism for catalysis. The lack of hairpin-stabilizing structural motifs is consistent with the absence of a hairpin intermediate in Mos1 excision. We have built a model for the DNA-binding domain of Mos1 transposase, based on the structure of the bipartite DNA-binding domain of Tc3 transposase. Combining this with the crystal structure of the catalytic domain provides a model for the paired-end complex formed between a dimer of Mos1 transposase and inverted repeat DNA. The implications for the mechanisms of first and second strand cleavage are discussed.


Subject(s)
DNA Transposable Elements , DNA-Binding Proteins/chemistry , Models, Molecular , Protein Conformation , Amino Acid Motifs , Amino Acid Sequence , Animals , Catalytic Domain , Crystallography, X-Ray , DNA-Binding Proteins/metabolism , Dimerization , Drosophila/enzymology , Manganese , Molecular Sequence Data , Plasmids , Protein Folding , Sequence Homology, Amino Acid , Spectrometry, Mass, Electrospray Ionization , Transposases
16.
Proc Natl Acad Sci U S A ; 101(41): 14719-24, 2004 Oct 12.
Article in English | MEDLINE | ID: mdl-15465912

ABSTRACT

Penelope-like elements are a class of retroelement that have now been identified in >50 species belonging to at least 10 animal phyla. The Penelope element isolated from Drosophila virilis is the only transpositionally active representative of this class isolated so far. The single ORF of Penelope and its relatives contains regions homologous to a reverse transcriptase of atypical structure and to the GIY-YIG, or Uri, an endonuclease (EN) domain not previously found in retroelements. We have expressed the single ORF of Penelope in a baculovirus expression system and have shown that it encodes a polyprotein with reverse transcriptase activity that requires divalent cations (Mn2+ and Mg2+). We have also expressed and purified the EN domain in Escherichia coli and have demonstrated that it has EN activity in vitro. Mutations in the conserved residues of the EN catalytic module abolish its nicking activity, whereas the DNA-binding properties of the mutant proteins remain unaffected. Only one strand of the target sequence is cleaved, and there is a certain degree of cleavage specificity. We propose that the Penelope EN cleaves the target DNA during transposition, generating a primer for reverse transcription. Our results show that an active Uri EN has been adopted by a retrotransposon.


Subject(s)
Drosophila/enzymology , Open Reading Frames/genetics , RNA-Directed DNA Polymerase/metabolism , Retroelements/genetics , Animals , Base Sequence , Conserved Sequence , Drosophila/genetics , Drosophila Proteins/genetics , Drosophila Proteins/metabolism , Endodeoxyribonucleases/genetics , Endodeoxyribonucleases/metabolism , Kinetics , Molecular Sequence Data , RNA-Directed DNA Polymerase/genetics
17.
Acta Crystallogr D Biol Crystallogr ; 60(Pt 5): 962-4, 2004 May.
Article in English | MEDLINE | ID: mdl-15103153

ABSTRACT

A soluble single-point mutant of full-length Mos1 mariner transposase (MW = 40.7 kDa) has been overexpressed in Escherichia coli, purified to 95% homogeneity and crystallized. This provides the first example of the crystallization of a eukaryotic transposase. The native crystals diffract to 2.5 A resolution and show tetragonal symmetry, with unit-cell parameters a = b = 44.5, c = 205.6 A. Multiple-wavelength anomalous data from a selenomethionyl form of the protein and data from a heavy-atom derivative have been collected.


Subject(s)
Drosophila Proteins/chemistry , Drosophila Proteins/genetics , Transposases/chemistry , Transposases/genetics , Amino Acid Sequence , Crystallization , Crystallography, X-Ray , Drosophila Proteins/isolation & purification , Escherichia coli/genetics , Molecular Sequence Data , Point Mutation , Protein Conformation , Recombinant Proteins/genetics , Recombinant Proteins/isolation & purification , Recombinant Proteins/metabolism , Selenomethionine , Transposases/isolation & purification
18.
Mol Cell ; 11(1): 225-35, 2003 Jan.
Article in English | MEDLINE | ID: mdl-12535535

ABSTRACT

It has been proposed that the modern immune system has evolved from a transposon in an ancient vertebrate. While much is known about the mechanism by which bacterial transposable elements catalyze double-strand breaks at their ends, less is known about how eukaryotic transposable elements carry out these reactions. We have examined the mechanism by which mariner, a eukaryotic transposable element, performs DNA cleavage. We show that the nontransferred strand is cleaved initially, unlike prokaryotic transposons which cleave the transferred strand first. First strand cleavage is not tightly coupled to second strand cleavage and can occur independently of synapsis, as happens in V(D)J recombination but not in transposition of prokaryotic transposons. Unlike V(D)J recombination, however, second strand cleavage of mariner does not occur via a hairpin intermediate.


Subject(s)
Bacteria/genetics , DNA-Binding Proteins/genetics , Drosophila melanogaster/genetics , Recombination, Genetic , Animals , Base Sequence , DNA/genetics , DNA/metabolism , DNA Transposable Elements , DNA-Binding Proteins/metabolism , Gene Rearrangement , Immune System/physiology , Nucleic Acid Conformation , Time Factors , Transposases
19.
Proc Natl Acad Sci U S A ; 99(25): 16150-5, 2002 Dec 10.
Article in English | MEDLINE | ID: mdl-12451171

ABSTRACT

The Penelope family of retroelements was first described in species of the Drosophila virilis group. Intact elements encode a reverse transcriptase and an endonuclease of the UvrC type, which may play a role in Penelope integration. Penelope is a key element in the induction of D. virilis hybrid dysgenesis, which involves the mobilization of several unrelated families of transposable elements. We here report the successful introduction of Penelope into the germ line of Drosophila melanogaster by P element-mediated transformation with three different constructs. Penelope is actively transcribed in the D. melanogaster genome only in lines transformed with a construct containing a full-length Penelope clone. The transcript is identical to that detected in D. virilis dysgenic hybrids. Most newly transposed Penelope elements have a very complex organization. Significant proliferation of Penelope copy number occurred in some lines during the 24-month period after transformation. The absence of copy number increase with two other constructs suggests that the 5' andor 3' UTRs of Penelope are required for successful transposition in D. melanogaster. No insect retroelement has previously been reported to be actively transcribed and to increase in copy number after interspecific transformation.


Subject(s)
Drosophila/genetics , Retroelements , Animals , Drosophila Proteins , Drosophila melanogaster/genetics , Female , Heterochromatin/genetics , Larva , Male , Organ Specificity , Species Specificity , Transcription, Genetic , Transformation, Genetic
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