ABSTRACT
SAMHD1 restricts lentiviruses by limiting availability of deoxynucleoside triphosphates for reverse transcription. HIV-2 and SIV have virion-associated proteins to counteract SAMHD1. Cats have an ortholog to human SAMHD1 and the FIV is restricted by human SAMHD1, but expression of feline SAMHD1 is unknown. Using a whole-body tissue microarray consisting of 24 tissues for immunohistochemistry, SAMHD1 expression was identified in a wide range of cat tissues. SAMHD1 was most strongly expressed in skin and mucosal epithelium, and in hemolymphatic and spermatogenic tissues. Both nuclear and cytoplasmic expression was detected. Feline cell lines susceptible to FIV infection also highly expressed SAMHD1. Preferential expression of SAMHD1 at sites of viral entry and replication supports a role for feline SAMHD1 in restricting FIV.
Subject(s)
SAM Domain and HD Domain-Containing Protein 1/metabolism , Sterile Alpha Motif , Animals , Blotting, Western/veterinary , Cats , Cell Line , Cell Nucleus/metabolism , Cytoplasm/metabolism , Flow Cytometry/veterinary , Intestinal Mucosa/metabolism , Male , Mononuclear Phagocyte System/metabolism , Reverse Transcriptase Polymerase Chain Reaction/veterinary , Sterile Alpha Motif/physiology , TranscriptomeABSTRACT
A 4-month-old kitten was presented with unilateral buphthalmos. The eye was blind with no menace response, but intraocular pressure was normal. A trans-palpebral enucleation was performed on the affected eye and the globe was submitted for histology. There was a suppurative, lympho-plasmacytic panophthalmitis with inflammatory exudate in the iridocorneal angle.
Buphthalmie unilatérale grave chez un chaton âgé de 4 mois. Un chaton âgé de 4 mois a été présenté avec de la buphtalmie unilatérale. L'Åil était aveugle sans réponse à la menace, mais la pression intraoculaire était normale. Une énucléation trans-palpébrale a été réalisée sur l'Åil affecté et le globe a été soumis pour examen histologique. Il y avait une panophtalmitie lympho-plasmacytique avec de l'exsudat inflammatoire dans l'angle irido-cornéen.(Traduit par Isabelle Vallières).
