ABSTRACT
22 patients with radiolucid stones and functioning gallbladder were grouped to establish ursodeoxycholic acid efficacy (i.e. gallstones dissolution) and innocuousness for gallbladder lithiasis. Methodological aspects were detailed, the dose determined (8 and 10 mg/k/d.) and the patients evaluated after a six months' treatment. 11 patients received "day-time" doses and the other 11 "overnight" dose. Only 7 subjects turned out to be evaluated for the study of biliary lipids, to the other 15 the development of their gallstones was followed up. Successful gallstone dissolution was achieved in 8 patients (53,3%), 2 reduced size and number. 5 out of these 8 patients received "day-time" doses and the other 3 "overnight" doses. From an attendance point of view, we do not consider biliary lipids study (bile cholesterol saturation rate) to be necessary.
Subject(s)
Cholelithiasis/drug therapy , Deoxycholic Acid/analogs & derivatives , Ursodeoxycholic Acid/therapeutic use , Cholesterol/metabolism , Drug Administration Schedule , Female , Follow-Up Studies , Humans , Male , Middle Aged , Ursodeoxycholic Acid/administration & dosageABSTRACT
Con la finalidad de establecer la eficacia (disolucion de calculo/s) e inocuidad del acido ursodesoxicolico en el tratamiento de la litiasis vesicular, se agruparon 22 pacientes con calculos radiolucidos y vesicula biliar funcionante. Se detallan aspectos metodologicos fijandose la dosis entre 8 y 10 mg./k/d evaluandose a los pacientes despues del sexto mes de tratamiento. 11 pacientes recibieron dosis "diurnas" y los 11 restantes dosis "nocturnas". 7 pacientes solo resultaron evaluables para el estudio de los lipidos biliares. De los 15 restantes a todos se les siguio la evolucion de sus calculos,8 (53,3%) disolvieron los mismos y en 2 se observo reduccion de numero y tamano. De esos 8 pacientes,5 recibieron dosis "nocturnas" y 3 "diurnas". Desde el punto de vista asistencial no creemos necesario el estudio de los lipidos biliares (indice de saturacion del colesterol biliar). Concluimos que el acido ursodesoxicolico resulta un farmaco util para tratar determinados casos de calculos vesiculares, sin haber observado reacciones adversas
Subject(s)
Cholelithiasis , Ursodeoxycholic AcidABSTRACT
Con la finalidad de establecer la eficacia (disolucion de calculo/s) e inocuidad del acido ursodesoxicolico en el tratamiento de la litiasis vesicular, se agruparon 22 pacientes con calculos radiolucidos y vesicula biliar funcionante. Se detallan aspectos metodologicos fijandose la dosis entre 8 y 10 mg./k/d evaluandose a los pacientes despues del sexto mes de tratamiento. 11 pacientes recibieron dosis "diurnas" y los 11 restantes dosis "nocturnas". 7 pacientes solo resultaron evaluables para el estudio de los lipidos biliares. De los 15 restantes a todos se les siguio la evolucion de sus calculos,8 (53,3%) disolvieron los mismos y en 2 se observo reduccion de numero y tamano. De esos 8 pacientes,5 recibieron dosis "nocturnas" y 3 "diurnas". Desde el punto de vista asistencial no creemos necesario el estudio de los lipidos biliares (indice de saturacion del colesterol biliar). Concluimos que el acido ursodesoxicolico resulta un farmaco util para tratar determinados casos de calculos vesiculares, sin haber observado reacciones adversas
Subject(s)
Ursodeoxycholic Acid , CholelithiasisABSTRACT
22 patients with radiolucid stones and functioning gallbladder were grouped to establish ursodeoxycholic acid efficacy (i.e. gallstones dissolution) and innocuousness for gallbladder lithiasis. Methodological aspects were detailed, the dose determined (8 and 10 mg/k/d.) and the patients evaluated after a six months treatment. 11 patients received [quot ]day-time[quot ] doses and the other 11 [quot ]overnight[quot ] dose. Only 7 subjects turned out to be evaluated for the study of biliary lipids, to the other 15 the development of their gallstones was followed up. Successful gallstone dissolution was achieved in 8 patients (53,3
), 2 reduced size and number. 5 out of these 8 patients received [quot ]day-time[quot ] doses and the other 3 [quot ]overnight[quot ] doses. From an attendance point of view, we do not consider biliary lipids study (bile cholesterol saturation rate) to be necessary.
ABSTRACT
To investigate ursodeoxycholic acid (Urso) action on liver histology, 30 male hamsters were allocated to 2 groups of 15 animals each; one group was treated with Urso, and the other one acted as control. Thirty days later, a light and electron microscopy study of the liver of all the animals was performed. All the group receiving Urso acid revealed minimal changes consisting in hepatocytic nuclear vacuolization around the centrelobulilla area; the vacuoles were homogeneous and contained a pale basofilic material. Neither inflammatory lesions nor hepatic cell necrosis was observed. Electron microscopy showed folding of the nuclear membrane corresponding to vacuoles observed under light microscopy. Further studies must be carried out to make clear the meaning of our findings and to confirm or otherwise rectify Urso inocuity on the liver of hamsters.
Subject(s)
Deoxycholic Acid/analogs & derivatives , Liver/drug effects , Ursodeoxycholic Acid/adverse effects , Animals , Cell Membrane/ultrastructure , Cricetinae , Liver/ultrastructure , Male , MesocricetusABSTRACT
To investigate ursodeoxycholic acid (Urso) action on liver histology, 30 male hamsters were allocated to 2 groups of 15 animals each; one group was treated with Urso, and the other one acted as control. Thirty days later, a light and electron microscopy study of the liver of all the animals was performed. All the group receiving Urso acid revealed minimal changes consisting in hepatocytic nuclear vacuolization around the centrelobulilla area; the vacuoles were homogeneous and contained a pale basofilic material. Neither inflammatory lesions nor hepatic cell necrosis was observed. Electron microscopy showed folding of the nuclear membrane corresponding to vacuoles observed under light microscopy. Further studies must be carried out to make clear the meaning of our findings and to confirm or otherwise rectify Urso inocuity on the liver of hamsters.
ABSTRACT
Para investigar la accion del acido ursodesoxicolico (Urso) sobre la histologia hepatica, 30 hamsters machos fueron agrupados en 2 lotes de 15 cada uno administrandosele a un grupo 39 mg./k/dia de Urso, sirviendo el otro lote como control. Despues de 30 dias se realizo estudio con microscopia de luz y electronica de todos los higados. En el grupo que recibio Urso se comprobo en un 100 x 100 de ellos cambios minimos consistentes en vacuolizacion nuclear de los hepatocitos del sector centrolobulillar; las vacuolas eran homogeneas y contenian un material debilmente basofilo. No se observaron lesiones inflamatorias ni necrosis hepatocelular. La microscopia electronica determino la presencia de invaginaciones de la membrana nuclear, correspondiente a las vacuolas observables con el microscopio de luz. Futuros estudios deberan realizarse para aclarar el verdadero significado de nuestros hallazgos y ratificar o no la inocuidad del Urso sobre el higado del hamster
Subject(s)
Deoxycholic Acid , LiverABSTRACT
Para investigar la accion del acido ursodesoxicolico (Urso) sobre la histologia hepatica, 30 hamsters machos fueron agrupados en 2 lotes de 15 cada uno administrandosele a un grupo 39 mg./k/dia de Urso, sirviendo el otro lote como control. Despues de 30 dias se realizo estudio con microscopia de luz y electronica de todos los higados. En el grupo que recibio Urso se comprobo en un 100 x 100 de ellos cambios minimos consistentes en vacuolizacion nuclear de los hepatocitos del sector centrolobulillar; las vacuolas eran homogeneas y contenian un material debilmente basofilo. No se observaron lesiones inflamatorias ni necrosis hepatocelular. La microscopia electronica determino la presencia de invaginaciones de la membrana nuclear, correspondiente a las vacuolas observables con el microscopio de luz. Futuros estudios deberan realizarse para aclarar el verdadero significado de nuestros hallazgos y ratificar o no la inocuidad del Urso sobre el higado del hamster