ABSTRACT
The results of the pharmacological investigation of the properties of apricot leaves dry extract are indicated in the article. It is proved that the extract belongs to the group of "relatively harmless" substances, exclusion of the ulcerogenic effect on the stomach, local irritating and allergenic effect on animals. It is found minimal reacting dose of the extract, which is 70 mg/kg of body weight. On the model of liver lesion by tetrachlormethane it is proved the antioxidant properties of the extract, which is manifested by the decreasing of the activity of oxidative processes and the resumption of the activity of the endogenous antioxidant system. At the studying of the bile formation and bile secretion functions in the conditions of the toxic tetrachlormethane lesion the hepatoprotective effect of the dosage form was confirmed, which was realized by the increasing of the speed of bile secretion and its volume. It is proved a positive effect of the extract on the detoxification function of the liver, that is confirmed by the reducing of the hexenal sleep in rats after toxicant exposure.
Subject(s)
Carbon Tetrachloride/toxicity , Chemical and Drug Induced Liver Injury/drug therapy , Plant Extracts/pharmacology , Protective Agents/pharmacology , Prunus/chemistry , Alanine Transaminase/metabolism , Animals , Catalase/metabolism , Chemical and Drug Induced Liver Injury/metabolism , Cholesterol/blood , Disease Models, Animal , Glutathione/blood , Guinea Pigs , Hydrazines/blood , Liver/drug effects , Liver/metabolism , Plant Leaves/chemistry , Rabbits , Rats , Superoxide Dismutase/metabolismABSTRACT
Four subcutaneous administrations of 2 g/kg of tetrachloromethane to albino rats inhibited the hepatic activity of superoxide dismutase, catalase, glutathione peroxidase, reduced the concentrations of tocopherol, retinol, ascorbic acid, glutathiones, decreased the plasma level of ceruloplasmin and the total antioxidative activity of liver tissue. The magnitude of changes in antioxidative parameters depended on the severity of hepatocytic destruction.
Subject(s)
Antioxidants/metabolism , Chemical and Drug Induced Liver Injury/metabolism , Animals , Catalase/metabolism , Glutathione Peroxidase/metabolism , Rats , Superoxide Dismutase/metabolismSubject(s)
Antioxidants/therapeutic use , Chemical and Drug Induced Liver Injury/drug therapy , Dimethyl Sulfoxide/therapeutic use , Oxidative Stress , Vitamin E/therapeutic use , Animals , Carbon Tetrachloride Poisoning/complications , Carbon Tetrachloride Poisoning/metabolism , Chemical and Drug Induced Liver Injury/metabolism , Dimethyl Sulfoxide/administration & dosage , Dimethyl Sulfoxide/pharmacology , Free Radicals , Male , Microsomes, Liver/drug effects , Microsomes, Liver/metabolism , Mitochondria, Liver/drug effects , Mitochondria, Liver/metabolism , Rats , Vitamin E/administration & dosage , Vitamin E/pharmacologyABSTRACT
The damage of the liver in albino rats induces by CCl4 was followed by the disorders in the free radical, microsomal, and mitochondrial oxidation. The administration of acetylcysteine to the animals in a dose of 500 mg/kg 3 times a day resulted in normalization of chemiluminescence parameters of the liver tissue, the rate of oxygen consumption by microsomes and mitochondria of the hepatocytes, and in normalization of the succinatdehydrogenase, cytochromooxidase, H-ATP-ase activity and ATP concentration.
Subject(s)
Acetylcysteine/therapeutic use , Carbon Tetrachloride Poisoning/drug therapy , Chemical and Drug Induced Liver Injury/drug therapy , Free Radical Scavengers/therapeutic use , Animals , Carbon Tetrachloride Poisoning/complications , Carbon Tetrachloride Poisoning/metabolism , Chemical and Drug Induced Liver Injury/etiology , Chemical and Drug Induced Liver Injury/metabolism , Drug Evaluation, Preclinical , Luminescent Measurements , Male , Microsomes, Liver/drug effects , Microsomes, Liver/metabolism , Mitochondria, Liver/drug effects , Mitochondria, Liver/metabolism , Oxidation-Reduction/drug effects , RatsABSTRACT
Liver injury with tetrachloromethane in white rats is accompanied by accumulation of hydroperoxides in the blood plasma and liver tissue and suppression of antioxidant system (the activity of superoxide dismutase, catalase, glutathione peroxidase, contents of SH-groups and total phospholipids drastically decreases). Ceruloplasmin level in plasma, on the contrary, increases. Enterosorbent SUGS-E in a dose of 19 g/kg partially normalizes violated balance between the activity of free radical processes and state of the antioxidant protection system.