Chlamydia trachomatis infection in women with CIN and invasive uterine cervix cancer. Significance of hormonal status.

In women with CIN at fertile age and those over 50 years of age, EGFR expression is lower in the presence of Chlamydia trachomatis (Cht) infection. In all Cht infected women over 50 years of age expression of Ki 67 is higher; the increase is significant among women with invasive carcinoma. In these groups of women with CIN and invasive carcinoma TGF-alpha expression is insignificantly augmented. Chronic Cht infection is associated with cervical hypertrophy.

Detection of Chlamydia trachomatis infection in women with CIN and invasive carcinoma. Controversial results of different methods.

Chlamydia (Ch.) trachomatis infection as a sexually transmitted disease is highly important, but reliable methods of diagnosing it remain to be worked out. We used three methods of detection: an immunoenzymatic technique for detection of Ch. trachomatis antigen in endocervical material, in situ PCR, and enzyme-immuno assay for detection of IgG class anti-Ch. trachomatis antibodies in serum. We have compared the IS-PCR technique and method of detection of the endocervical antigen. We have not confirmed compatibility of the results obtained in these two methods. Parallel positive results obtained in patient serum and detection of chlamydial DNA by IS-PCR have been accepted to be indicative of persistent infection of Ch. Trachomatis.

Estimation of the usefulness of neoplastic markers TPS and CA 125 in diagnosis and monitoring of ovarian cancer.

PURPOSE: We have undertaken an attempt to compare the suitability of tumor markers TPS (tissue polypeptide specific antigen) and CA125 for diagnosis and monitoring of ovarian cancer patients. METHODS AND MATERIAL: The studies were performed on 33 patients treated for ovarian cancer in the Department of Oncology, Karol Marcinkowski School of Medicine, Poznan from 1995-1996, Serum levels of TPS and CA125 were determined before surgery and at each chemotherapy course. CONCLUSION: Estimation of the neoplastic markers TPS and CA125 is suitable for diagnosis of ovarian cancer. Parallel use of TPS and CA125 in ovarian cancer patients increases sensitivity of the diagnosis. Estimation of TPS is highly suitable and estimation of CA125 is of low value in detection of mucinous ovarian cancer. Serum levels of the neoplastic markers TPS and CA125 decrease after total or debulking surgery for ovarian cancer. Serum TPS and CA125 levels reflect the course of the neoplastic process during chemotherapy.

The role of Chlamydia trachomatis infection in cervical cancer development.

Previous Chlamydia trachomatis infection elevates expression of: 1. TGFalpha in CIN I, CIN II, CIN III; 2. HPV 16 in CIN I, CIN II, CIN III and invasive carcinoma; 3. Ki 67 in CIN III and invasive carcinoma. Chronic Chlamydial infection is very often associated with cervical hypertrophy.

[Studies on DNA content in sperm of men with oligozoospermia]. / Badania nad zawartoscia DNA w plemnikach mezczyzn z oligozoospermia.

The aim of our study was to analysis the ploidy of spermatozoa from oligozoospermic individuals. The study was performed in 48 patients with oligozoospermia. Spermatozoal DNA was analysed using MagiCal imaging cytometry. The percentage of aneuploid cells was determined using CYT-2 program. The results indicated aneuploidy in 73% of the examined patients. The amount of cells with abnormal DNA content ranged from 30 to 100%. Monitoring of the DNA in human spermatozoa may have practical applications in establishing the reasons for infertility as well as for screening of the semen for assisted reproduction techniques.

Studies of the effect of simulated acid rain on the ecological equilibrium of the soil system.

Introduction to the problem of environmental pollution caused by acid depositions. Presentation of principles of the six-year field experiment with simulated acid rain. Short characteristics of particular parts of the series of papers.

Termination of normal and pathological pregnancy with Sulprostone.

262 patients with normal pregnancy in the first and second trimester and 55 patients with pathological pregnancy (missed abortion, intrauterine death of the foetus, molar pregnancy) were successfully treated with the PGE2 analogue Sulprostone (Schering A. G.). The drug was administered intramuscularly in the first trimester of normal and pathological pregnancy and by constant intravenous drop infusion for induction of abortion in the second and third trimesters of pregnancy. In case of 38 patients Sulprostone was injected intramural-cervically for cervical dilatation. The efficacy was high and the incidence of side-effects was low and more acceptable in all groups compared with that after natural prostaglandins. On the basis of the results presented, the authors recommend for pregnancy termination with Sulprostone the intramuscular route in the first trimester of normal and pathological pregnancy and intravenous infusion in the second and third trimesters.

PIP: 262 patients with normal pregnancy in the 1st and 2nd trimester and 55 patients with pathological pregnancy (missed abortion, intrauterine death of the fetus, molar pregnancy) were treated with the prostaglandin E2 analogue Sulprostone to evaluate the safety, effectiveness, and acceptability of this drug to induce abortion in the 1st and 2nd trimesters of normal and pathological pregnancy as well as to dilate the cervix prior to surgical artificial abortion. The patients, ranging in age from 16-38 years, were admitted to the Institute of Obstetrics and Gynecology of the Medical Academy of Poznan, Poland. All patients volunteered to participate in this study. Among those patients admitted because of artificial abortion, duration of amenorrhea ranged from 6-12 weeks. The patients were divided into 3 groups. To the 23 patients in the 1st group, Sulprostone was given 2-3 times 500 mcg intramuscularly at 3-6 hour intervals to induce abortion. Curettage was performed after abortion or within 24 hours when abortion failed to occur. In the 2nd group of 10, Sulprostone was used for pharmacological cervix dilatation prior to curettage. 25 mcg of the drug was administered intramuscular-cervically to 32 patients 12 hours before surgical evacuation of cavum uteri. In 191 pregnancies Sulprostone was administered intramuscularly with 1 dose of 500 mcg also 12 hours prior to curettage. In the group of missed abortion (n = 23), 1-3 doses of 500 mcg were injected intramuscularly. In the case of 10 patients admitted for therapeutic abortion in the 2nd trimester group of intrauterine fetal death, a total dose of 1000-1500 mcg of Sulprostone was infused for over 12 hours. The incidence of gastrointestinal side effects in all groups was limited and clinically acceptable. In the case of induced uterine pains, the analgesic Dolantin was offered on an as-needed basis. Systemic side-effects requiring discontinuation of therapy with Sulprostone were not observed. The 262 patients with normal pregnancy and the 55 patients with pathological pregnancy were treated successfully with Sulprostone. The intramuscular administration seems to be preferable in the 1st trimester for cervical dilatation and other indications and intravenous infusion in the 2nd and 3rd trimesters.

Inaccuracy of house staff in reading throat cultures.

The low prevalence of streptococcal pharyngitis in our pediatric clinic during a normally high-prevalence period encouraged us to examine the accuracy of throat culture readings made by the house staff. Six pediatric residents processed 256 cultures, which were then reinterpreted by a microbiologist. The residents consistently underread the cultures, failing to identify 68 (60%) of 112 with beta-hemolysis and 23 (58%) of 40 with a zone of bacitracin inhibition. This inaccuracy in interpretation of throat cultures emphasizes the physician's need to periodically reassess these skills.