ABSTRACT
The purpose of this study was to evaluate in vitro the effectiveness of two endodontic irrigants and their association against Enterococcus faecalis (E. faecalis) by confocal laser scanning microscope (CLSM). Twenty-four bovine incisors were inoculated in a monoculture of E. faecalis for 21 days. After this period, the teeth were divided into three test groups (n = 5) according to the chemical used. Group 1: 2.5% sodium hypochlorite (NaOCl), group 2: 2% chlorhexidine gel (CHX), group 3: 2.5% NaOCl + 2% CHX gel, and two control groups (n = 3): negative control group (NCG)-sterile and without root canals preparation and positive control group (PCG)-saline. Then, the samples were stained with SYTO9 and propidium iodide and subjected to analysis by CLSM. Bacterial viability was quantitatively analyzed by the proportions of dead and live bacteria in the biofilm remnants. Statistical analysis was performed by the One-way ANOVA test (p = 0.05). No statistical differences were observed to bacterial viability. According to CLSM analysis, none of the tested substances could completely eliminate E. faecalis from the root canal space. Until now, there are no irrigant solutions able to completely eliminate E. faecalis from the root canal. In this regard, the search for irrigants able to intensify the antimicrobial action is of paramount importance.
Subject(s)
Biofilms/drug effects , Enterococcus faecalis/drug effects , Enterococcus faecalis/physiology , Incisor/microbiology , Microscopy, Confocal , Root Canal Irrigants/administration & dosage , Animals , Cattle , Microbial Viability/drug effects , Organic Chemicals , Propidium , Staining and LabelingABSTRACT
O objetivo do presente estudo foi avaliar as competências em relação às Tecnologias de Informação e Comunicação (TICs) de estudantes de Odontologia. Um estudo transversal foi realizado com os estudantes de Odontologia da Universidade Federal do Rio Grande do Sul, Brasil. Os dados foram coletados através de um questionário previamente validado pelo Centro de Ciências de Saúde Oral da Universidade de Malmö e submetido ao processo de adaptação transcultural para o português. Os resultados foram transformados em escores conforme literatura prévia e categorizados para as comparações. Análise descritiva, qui-quadrado e teste exato de Fischer foram utilizados. O escore de competência em relação às TICs foi considerado muito bom e apresentou relação com o nível de escolaridade materno. Já o escore de importância das TICs foi mediano e relacionado à idade. Concluiu-se que os estudantes de Odontologia apresentaram bom domínio das ferramentas de TICs e nível moderado em relação à importância atribuída ao uso odontológico dessas tecnologias (AU).
The purpose of the present study was to evaluate the competence of the students to use technologies in dental educational. A cross-sectional study was done with dental students of a university of the south of Brazil. It was used a questionnaire previously validated by the Center Oral Health Sciences of University of Malmö (Sweden) and adapted to Portuguese. The collected data were transformed into scores based on Mattheos et al. (2005). For analysis purposes, scores and sociodemographic variables were categorized. The descriptive analyses was realized, Chi Square and Exact Fischer`s tests were used to test the association between the sociodemographic data and the scores regarding the Information and Communication Technologies (ICT). The mean competence score was considered very good and associated with education level of the mothers. The mean importance score was regular and associated with age. It was concluded that the students of a university of south of Brazil had a very good level regarding the competence of the use of ICT even though moderate level in relation to the importance was given to dental use of these technologies (AU).
